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Volume 28 (1984)
- Issue 6 Pages 333-
- Issue 5 Pages 280-
- Issue 4 Pages 195-
- Issue 3 Pages 149-
- Issue 2 Pages 67-
- Issue 1 Pages 7-
Volume 28, Issue 4
Displaying 1-13 of 13 articles from this issue
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Use of electrophoretic techniques in purification and analysis of proteinsKaoru Miyazaki, Keisuke Mashima, Nobuhiko Yamashita, Yasunori Kihira, ...1984 Volume 28 Issue 4 Pages 195-200
Published: August 30, 1984
Released on J-STAGE: March 31, 2009
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Zen-ichi Ogita1984 Volume 28 Issue 4 Pages 201-206
Published: August 30, 1984
Released on J-STAGE: March 31, 2009
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Gene of aldolase isozymeKatsuji Hori, Tsunehiro Mukai, Kei-ichiro Joh, Minoru Sakakibara, Kiic ...1984 Volume 28 Issue 4 Pages 207-212
Published: August 30, 1984
Released on J-STAGE: March 31, 2009
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Human gene mappingMichihiro C. Yoshida1984 Volume 28 Issue 4 Pages 213-222
Published: August 30, 1984
Released on J-STAGE: March 31, 2009
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Enzyme polymorphism in manChiyoko Satoh1984 Volume 28 Issue 4 Pages 223-229
Published: August 30, 1984
Released on J-STAGE: March 31, 2009
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A study on isozyme genes of DrosophilaTadashi Aotsuka, Yoko Aigaki, Yuji Taka, Jun-ichiro Murata, Shigeru Oh ...1984 Volume 28 Issue 4 Pages 231-236
Published: August 30, 1984
Released on J-STAGE: March 31, 2009
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With an emphasis on lactate dehydrogenase isozymeTakashi Kanno1984 Volume 28 Issue 4 Pages 237-242
Published: August 30, 1984
Released on J-STAGE: March 31, 2009
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Present and future prospectsTakashi Kanno1984 Volume 28 Issue 4 Pages 243
Published: August 30, 1984
Released on J-STAGE: March 31, 2009
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1) Basic investigations and protein fractions of normal urineTomiko Harada, Kaoru Hatsusegawa, Chizuko Mori, Kiyoko Kanamori, Kiyok ...1984 Volume 28 Issue 4 Pages 245-250
Published: August 30, 1984
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSFor the purpose of studying urinary proteins electrophoretically, a highly sensitive silver staining method was applied to urinary protein fractions separated by SDS-polyacrylamide gel electrophoresis. With this new method, urine specimens from normal human subjects, containing about 10mg/dl protein, could be separated into 26 protein bands without any pretreatment. Proteins having molecular weights from 230, 000 to 15, 000 were included in these bands. Three protein bands with molecular weights of 94, 000, 64, 000 and 15, 000 were found to be present in all of the 27 urine specimens from normal human subjects, while two protein bands with molecular weights of 82, 000 and 47, 000 were detected in 22 cases (80%). The protein bands with molecular weights of 94, 000, 64, 000 and 15, 000 were identified by immunoblotting technique to be Tamm mucoprotein, albumin and, β2-microglobulin, respectively.View full abstractDownload PDF (7095K) -
Taizo Hayashi, Takao Tanaka1984 Volume 28 Issue 4 Pages 251-256
Published: August 30, 1984
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSChanges of enzyme activities and isozyme patterns in the hypoxic states were studied using heart muscles, liver and blood of rats. Plasma isozyme patterns rapidly changed in the recovery stage after returning from hypoxia. At the maximal hypoxic stage, plasma LDH-isozyme patterns were similar to those of the liver, however, after returning from hypoxia, the LDH-isozyme patterns quickly approached the pattern of the heart muscles with increasing LDH1 and LDH2. In plasma CK-isozymes, prompt decreases of CK-BB, increases of CK-MB and disappearance of mitochondrial CK were observed in the recovery stage after returning from hypoxia, while mitochondrial CK was usually observed in hypoxia.
Isozyme patterns of LDH and CK in the blood reflect subcellular structure and condition of the cells which release these enzymes. In hypoxia, the changes of arterial oxygen content brought direct effects upon the blood isozyme patterns during alteration of the blood flow in the organs.View full abstractDownload PDF (3877K) -
In relation to abnormal bands seen in M-proteinemiaYosuke Miyake, Takao Miura, Kei Miyamoto1984 Volume 28 Issue 4 Pages 257-260
Published: August 30, 1984
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSA simple method for fractionation and detection of serum sialoproteins is described. The method is based on the combination of cellulose acetate membrane electrophoresis and sialic acid staining using enzyme reactions of neuraminidase NANA-aldolase, pyruvate oxidase and peroxidase.
The results obtained are as follows:
1) Total serum sialic acid in 52 healthy Japanese ranged from 53 to 73mg/dl, and serum sialoproteins were separated into fractions with α1-, α2- and β-globulin mobilities, the relative contents (mean±2SD) being 39±14, 40±16 and 20±14%, respectively.
2) Another band of sialoprotein was found in position of the electrophoretic mobility of M-protein in some cases of M-proteinemia (IgG, IgA, IgM and B-J).
The method may be useful for screening test of qualitative analysis of serum sialoproteins.View full abstractDownload PDF (3222K) -
Eiji Fujii, Keiko Kawajiri, Masakazu Minowa, Kiyomi Akaishi, Ikunosuke ...1984 Volume 28 Issue 4 Pages 261-266
Published: August 30, 1984
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSRecently, silver staining has been used for the detection of a trace amount of protein using agarose gel electrophoresis. However, some problems, such as easy contamination of the background by nonprotein substances and necessity of complicated steps and skillful technique for staining have made it less satisfactory. A more simplified method is necessary for routine work. Therefore, we modified successfully the silver staining method published by Kerényi et al.
The present improved silver staining method gave the same sensitivity as Kerényi's method, and yet the procedure was simplified and the staining reproducibility was satisfactory. It is also useful for the detection of oligoclonal bands in cerebrospinal fluid from the patients with demyelinating diseases and various types of viral infections, when it is combined with high resolution agarose gel.View full abstractDownload PDF (3590K) -
Makoto Watanabe, Hideharu Yamashita, Yoshihiro Shimada1984 Volume 28 Issue 4 Pages 267-269
Published: August 30, 1984
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSQuantitative measurement of a cholestatic lipoprotein, Slow-migrating HDL(HDL-S), was successfully attempted in a serum from a patient with biliary obstruction by crossed electrophoresis from polyacrylamide gel into agarose gel followed by cholesterol staining.View full abstractDownload PDF (3166K)
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