A single dose of 5, 30 or 60μg/kg of 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin (TCDD) reduced the thymus weight in a dose-dependent manner in female, 7-week-old C57BL/6 mice. The size and cellularity of the thymic cortex were reduced sharply on Day 3 after TCDD (30μg/kg) treatment, reached at a nadir on Day 7, and recovered to control levels on Day 35. TCDD (30μg/kg) treatment induced a significant decrease in the number and percentage of the L3T4
+Ly-2
+ and the PNA
+ cells in parallel with a thymic cortical atrophy with thymocyte depletion. Concurrently, there was a proportional increase in the percentage of the L3T4
+Ly-2
-, L3T4
-Ly-2
+ and L3T4
-Ly-2
- subsets. The absolute number of each subset was decreased along with the reduction in the total thymocyte number. A slight but insignificant decrease in the number of the L3T4
-Ly-2
+ cells continued by Day 14, despite restoration of the other three subsets. Unlike the thymocytes, there were no appreciable changes in each subpopulation of spleen cells, a few exceptions being a decreased Thy-1
+ and an increased sIg
+ cells on Day 3. In TCDD-treated mice that were immunized with SRBC on Day 3 or 7 posttreatment, the 4-day IgM plaque-forming cell (PFC) response was markedly impaired, whereas the delayed footpad response to SRBC was unaffected. By utilizing the modified Mishell-Dutton culture system, it was demonstrated that in vivo exposure to TCDD produced suppression of PFC induction by affecting B cells, perhaps directly, without affecting helper T cell function.
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