Effects of sulfate load on bacterial propionate oxidation were examined in anaerobic sludge that had been acclimated with glucose as a carbon source for more than 1 month under each sulfate loads (0-0.475 mmol SO
42- ·1
-1·day
-1). Bacterial propionate oxidation rate in no-sulfate load sludge was 0.595 mmol·1
-1·hr
-1, but in the sludge of sulfate load of 0.475 mmol SO
42- ·1
-1·day
-1, propionate oxidation rate was 0.814 mmol · l
-1·hr
-1. Chloroform, a specific inhibitor of methanogenesis, completely inhibited propionate oxidation in no-sulfate load sludge. This suggests that H
2- producing propionate oxidizing bacteria was also inhibited by accumulation of H
2. owever, in the sulfate load sludge (0.118 mmol SO
42- ·1
-1·day
-1), propionate oxidation rate was decreased by chloroform to only 67% of that without chloroform. Molybdate, a specific inhibitor of sulfate reduction, decreased propionate oxidation rate to 58% in sulfate load sludge (0.475 mmol SO
42- ·1
-1·day
-1). These results show that propionate oxidation was stimulated by incompletely propionate-oxidizing sulfate reducers when the sulfate load was increased. Also, sulfate reducers competed for propionate with H
2-producing propionate oxidizing bacteria in the high sulfate load sludge.
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