There is little information on environmental hazard of nanomaterials due to its unique physicochemical properties and biological interactions. In the present study, acute toxicity tests for fullerene C
60 using aquatic organisms were conducted to investigate the effects of the substance on the aquatic ecosystem. An aqueous suspension of fullerene C
60 (500 mg/L) which included nano-sized particles was prepared by grinding with sugar candy and polyoxyethylene hydrogenated castor oil for the tests. A 96-h acute toxicity test with medaka (Oryzias latipes), a 48-h acute immobilization test with Daphnia magna and a 72-h algae growth inhibition test with Pseudokirchneriella subcapitata were conducted using this aqueous suspension of fullerene C
60 according to the existing OECD test guidelines . As a result, the 96-h LC50 (median lethal concentration) for medaka, the 48-h EC50 (median effective concentration) for D. magna and 72-h EC50 for algae based on the growth rate (0-72 h) were estimated to be larger than 2.15, 2.25 and 2.27 mg/L, respectively. However, in the algae test, the growth rate (0-72 h) was significantly inhibited in the groups exposed to 0.0551-mg/L and higher concentrations of fullerene C
60. This result indicated that the 72-h NOEC (no observed effect concentration) based on the growth rate (0-72 h) was estimated to be 0.0178 mg/L.
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