The relation between oleate and biotin to the extracellular accumulation of L-GA in
M. ammoniaphilum was studied, and the follow-ing results were obtained and discussed.
1.
M. ammoniaphilum was cultured in glucose medium at various biotin levels, and the cellular fatty acids were analyzed by gas chromatography.
From the results of this experiment, the following possible diagram was proposed;
Amount of Biotin Added → Amounts of Clg
1=18 and/or C
16→Amounts of Cells Formed… (l) In this diagram, [A→B] means [A was the limiting factor of B formation].
2. Through the addition of various fatty acids to the fermentation medium, the second
step of the diagram (l) was proved to hold good, independently of the first step of the diagram (l) as follows,
Amount of C
1=8 →Amount of cells formed. In connection with this, the biotin-sparing effect of oleate was proven to be negligible by transferring the bacteria into oleate medium, and also shown that oleate could substitute for biotin and cystine which were indispens-able for the growth of the bacteria.
3.
M. ammoniaphilum was also cultured in glucose-oleate medium, and the cellular fatty acids were analyzed.
From the results of this experiment, it was recognized that a large amount of L-GA was accumulated, while the ratio of Sat./Unsat. F. A. was far below 1.
4. 0.05% Na-oleate was added supplement-arily to the molasses-POEFE medium and glucose-oleate medium at 12hr. of culture, and it was found that the extracellular ac-cumulation of L-GA practically stopped in either medium after the addition.
It was suggested from this fact that the same permeability mechanism for L-GA was work-ing in both cases.
5. The cellular permeability was investi-gated with the cells acquired in the preceding experiments, and it was recognized that the better the cellular permeability of L-GA, the larger the amount of L-GA accumulated.
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