Agricultural and Biological Chemistry
Online ISSN : 1881-1280
Print ISSN : 0002-1369
ISSN-L : 0002-1369
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Volume 26, Issue 3
Displaying 1-20 of 20 articles from this issue
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  • Studies on Oxidative Fermentation
    Part XX. Catabolism of 5-Ketogluconate by Gluconobacter liquefaciens. I
    Haruyoshi MUROOKA, Yukashi KOBAYASHI, Toshinobu ASAI
    1962 Volume 26 Issue 3 Pages 135-141
    Published: 1962
    Released on J-STAGE: February 07, 2011
    DOIhttps://doi.org/10.1271/bbb1961.26.135
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    It has been found that Gluconobacter liquefaciens metabolized 5-ketogluconic acid. In order to clarify metabolic pathways of this compound, the oxidation products by resting cells of this organism were investigated. Rubiginol, rubiginic, comenic, 2, 5-diketogluconic, glycolic and tartronic acids were detected or identified in the reaction fluid. On the basis of these results and the data obtained by means of manometric experiments, the oxidation pathways of 5-ketogluconic acid were discussed.
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  • Studies on Oxidative Fermentation
    Part XXI. Catabolism of 5-Ketogluconate by Gluconobacter liquefaciens. II
    Haruyoshi MUROOKA, Yukashi KOBAYASHI, Toshinobu ASAI
    1962 Volume 26 Issue 3 Pages 142-149
    Published: 1962
    Released on J-STAGE: February 07, 2011
    DOIhttps://doi.org/10.1271/bbb1961.26.142
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    Oxidation pathways of 5-ketogluconic acid by resting cells of Gluconobacter liquefaciens were further investigated. Arsenite inhibited the oxidation of this compound. The amount of carbonyl compounds in the oxidation products of 5-ketogluconic acid was increased by addition of 10-3M arsenite. Pyruvic and α-ketoglutaric acids were identified among these carbonyl compounds. Members of the tricarboxylic acid cycle were oxidized actively by resting cells or cell-free extracts of this organism. These results suggested the presence of the tricarboxylic acid cycle in the terminal oxidation of 5-ketogluconic acid by this organism.
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  • Chemical and Biological Studies on the Formulation of Antibiotics and Organic Mercurial Compound
    Part I. Investigation on Compatibility of Blastcidin S and Phenylmercuric Acetate
    Yoshio ISHII
    1962 Volume 26 Issue 3 Pages 150-152
    Published: 1962
    Released on J-STAGE: February 07, 2011
    DOIhttps://doi.org/10.1271/bbb1961.26.150
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    During the course of investigation on the formulation of Blastcidin S and PMA, it was found that some types of surface active agents and lignin sulfate gave undesirableinfluences to Blastcidin S, while lignin sulfate had a bad effect upon PMA.
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  • Chemical and Biological Studies on the Formulation of Antibiotics and Organic Mercurial Commpound
    Part II. Effects of the Combination of Blastcidin S and PMA Against Rice Blast, Piricularia oryzae
    Yoshio ISHII
    1962 Volume 26 Issue 3 Pages 153-155
    Published: 1962
    Released on J-STAGE: February 07, 2011
    DOIhttps://doi.org/10.1271/bbb1961.26.153
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    Twenty ppm Blastcidin was necessary to exert influence on rice blast, while PMA required 20ppm (Hg) for protective purpose. The combination of Blastcidin S and PMA with half concentration each was shown to be superior to that of separate application on both therapeutic and protective effects.
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  • Studies on the Tannin of Japanese Persimmon (Diospyros Kaki L.)
    Part I. Isolation of Leucoanthocyanin from Kaki Fruit
    Saburo ITO, Yasuyoshi OSHIMA
    1962 Volume 26 Issue 3 Pages 156-161
    Published: 1962
    Released on J-STAGE: February 07, 2011
    DOIhttps://doi.org/10.1271/bbb1961.26.156
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    Kaki tannin was isolated from methanol extracts of unripe kaki fruit (Japanese persimmon) and it was found that a main constituent composing kaki tannin was a leucoanthocyanin. Its acetate and methyl ether were prepared and their properties and reactions were studied. From the results of these experiments, kaki tannin was presumed to be a (+) leucodelphinidin-3-glucoside (5, 7, 3', 4', 5'-pentahydroxyflavan-3, 4-diol-3-glucoside).
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  • Studies on the Pentose Isomerases of Lactic Acid Bacteria
    Part VI. Effect of Metals on Isomerase Production
    Kei YAMANAKA, Takanori HIGASIHARA
    1962 Volume 26 Issue 3 Pages 162-166
    Published: 1962
    Released on J-STAGE: February 07, 2011
    DOIhttps://doi.org/10.1271/bbb1961.26.162
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    Production of D-xylose and L-arabinose isomerases by lactic acid bacteria was greatly promoted by the addition of manganese ions in cultural medium. Effective concentration of the ions was 5×10-3M. Ferrous ions were also effective for the production of D-xylose isomerase and cobaltous ions were somewhat effective for the production of L-arabinose isomerase. Zinc and cadmium ions inhibited bacterial growth. It was possible to increase the production of isomerase by changing MnSO4 concentration to 5×10-3M (0.11%) in place of 0.001 per cent in the normal medium.
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  • Studies on the Pentose Isomerases of lactic Acid Bacteria
    Part VII. Chromatography of Pentose Isomerases
    Kei YAMANAKA
    1962 Volume 26 Issue 3 Pages 167-174
    Published: 1962
    Released on J-STAGE: February 07, 2011
    DOIhttps://doi.org/10.1271/bbb1961.26.167
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    Column chromatographic procedures for the purification of pentose isomerases were carried out. Cation and anion exchange resins were not suitable because of their low exchange capacities and instability of the enzyme at acidic pH range. But the isomerases were successfully puri d by DEAE-cellulose column chrorrlatography with high recovery (85-90%). Using a Tris buffer, KCl concentration was increased in gradient. D-Xylose isomerase was eluted at pH 7.0 at 0-0.2M KCl, and L-arabinose isomerase at pH 8.0 at 0-0.4M KCl. The purified isomerases, D-xylose isomerase and L-arabinose isomerase, both required manganese ionsspecifically for their activities.
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  • Studies on the Pentose Isomerases from Lactic Acid Bacteria
    Part VIII. Separation of D-Xylose and L-Arabinose Isomerases
    Kei YAMANAKA
    1962 Volume 26 Issue 3 Pages 175-179
    Published: 1962
    Released on J-STAGE: February 07, 2011
    DOIhttps://doi.org/10.1271/bbb1961.26.175
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    D-Xylose isomerase and L-arabinose isomerase are different enzymes which can be separated from each other with acetone fractionation at pH 4.8-5.0, heat treatment or chromatography on a column of DEAE-cellulose. In DEAE-cellulose chromatography with a linear gradient elution method, D-xylose isomerase is recovered in the first peak at pH 7.0 Trisbuffer with 0-0.2M KCl, and Farabinose isomerase is eluted in the second peak at pH 83 (Tris buffer) with a larger ionic strength.
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  • Components of Unsaponifiable Matter of Rice Bran Oil
    Toshimi AKIYA
    1962 Volume 26 Issue 3 Pages 180-186
    Published: 1962
    Released on J-STAGE: February 07, 2011
    DOIhttps://doi.org/10.1271/bbb1961.26.180
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    Components of the unsaponifiable matter of crude rice bran oilwere examined. Anew compound having melting point of 157-158°C was obtained. Molecular weight was determined and ultraviolet and infrared analyses were carried out. Squalene and ferulic acid ester were confirmed to exist in the unsaponifiable matter of crude rice bran oil. Quantitative relation of components was calculated and found to be 42% sterols, 24% higher alcohols, 20% ferulic acid esters, 10% hydrocarbons and 2% unreported compound.
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  • Chemical Studies on Amino-Carbonyl Reaction
    Part I. Isolation of 3-Deoxypentosone and 3-Deoxyhexosones Formed by Browning Degradation of N-Glycosides
    Hiromichi KATO
    1962 Volume 26 Issue 3 Pages 187-192
    Published: 1962
    Released on J-STAGE: February 07, 2011
    DOIhttps://doi.org/10.1271/bbb1961.26.187
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    The 3-deoxy-D-pentosone (I) was isolated from the browning degradation mixture of N-D-xylosyl-n-butylamine by the action of acetic acid at 55°C. The 3-deoxy-D-erythrohexosone (IIa) and the 3-deoxy-D-threohexosone (IIb) were also prepared by degradation of the corresponding N-glycosyl-n-butylamine. The 3-deoxy-D-pentosone was characterized as the 2, 4-dinitrophenylosazone and its diacetate, and the p-nitrophenylosazone. The two 3-deoxy-D-hexosones were also characterized as the analogous derivatives. The three 3-deoxyosones gave positive color reactions with 2-thiobarbituric acid.
    As one of the intermediates in 3-deoxyosone formation from N-glycoside, 1, 2-enol form of 1-deoxy-1-n-butylamino-2-ketose (IV) was proposed.
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  • Studies on the ε-Lysine Acylase
    Part IV. Cultural Conditions for Production of ε-Lysine Acylase in Achromobacter pestifer EA
    Tsutomu ISHIKAWA, Tetsuya TOSA, Ichiro CHIBATA
    1962 Volume 26 Issue 3 Pages 193-198
    Published: 1962
    Released on J-STAGE: February 07, 2011
    DOIhttps://doi.org/10.1271/bbb1961.26.193
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    Since Achromobacter pestifer EA isolated from soils shows markedly high ε-lysine acylase activity compared with those of the other microorganisms ever tested, cultural conditions for the production of this enzyme were investigated.
    As a result, it was confirmed that simple medium containing 1% peptone, 5% glucose and some inorganic salts is most suitable for the enzyme production and that much moreε-lysine acylase is produced by shaken culture or submerged culture in jaderrrlentor than by stationary culture.α-Amino acylase activity in this organism was also studied.
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  • Nematocidal Action of Microorganisms
    Hiroshi IIZUKA, Kazuo KOMAGATA, Teinosuke KAWAMURA, Yoshiaki KUNII, Ma ...
    1962 Volume 26 Issue 3 Pages 199-200
    Published: 1962
    Released on J-STAGE: February 07, 2011
    DOIhttps://doi.org/10.1271/bbb1961.26.199
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  • The Modification of the Ungerminated Barley Endosperm with Gibberellin
    Harugoro YOMO, Hironobu IINUMA
    1962 Volume 26 Issue 3 Pages 201
    Published: 1962
    Released on J-STAGE: February 07, 2011
    DOIhttps://doi.org/10.1271/bbb1961.26.201
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  • ERRATA
    1962 Volume 26 Issue 3 Pages e1a
    Published: 1962
    Released on J-STAGE: February 07, 2011
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  • ERRATA
    1962 Volume 26 Issue 3 Pages e1b
    Published: 1962
    Released on J-STAGE: February 07, 2011
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  • ERRATA
    1962 Volume 26 Issue 3 Pages e1c
    Published: 1962
    Released on J-STAGE: February 07, 2011
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  • ERRATA
    1962 Volume 26 Issue 3 Pages e1d
    Published: 1962
    Released on J-STAGE: February 07, 2011
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  • ERRATA
    1962 Volume 26 Issue 3 Pages e1e
    Published: 1962
    Released on J-STAGE: February 07, 2011
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  • ERRATA
    1962 Volume 26 Issue 3 Pages e1f
    Published: 1962
    Released on J-STAGE: February 07, 2011
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  • ERRATA
    1962 Volume 26 Issue 3 Pages e1g
    Published: 1962
    Released on J-STAGE: February 07, 2011
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