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Volume 27, Issue 6
Displaying 1-14 of 14 articles from this issue
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Susumu OI, Yukio SATOMURA1963 Volume 27 Issue 6 Pages 397-404
Published: 1963
Released on J-STAGE: February 07, 2011
JOURNAL FREE ACCESSThe phospholipase of Sclerotinia fungus was separated by the paperelectrophoresis method into three fractions, of which one component is phospholipase A and the others phospholipase B. These enzymes could be isolated also by the column chromatography using anion exchange resin Duolite A 2 and DEAE cellulose. The former and one of the latter enzymes were obtained in a crystalline form from its solution saturated with ammonium sulfate. The investigation of substrate specificities of these enzymes has shown that the two phospholipase B hydrolyze a commercial soybean lecithin, and one of them can also attack egg-yolk lecithin without addition of any activating lipides.View full abstractDownload PDF (1646K) -
Susumu OI1963 Volume 27 Issue 6 Pages 405-408
Published: 1963
Released on J-STAGE: February 07, 2011
JOURNAL FREE ACCESSBy using the purified phospholipase A and B of Sclerotinia Libertiana Fcl., enzymic degradation of soy-lecithin was investigated. From the paperchromatography experiments, it was concluded that the phospholipase A specifically hydrolyzes the ester linkage of unsaturated fatty acid of soy-lecithin whereas the phospholipase B hydrolyzes the linkage of saturated acid of soy-lysolecithin. Phospholipase B also could hydrolyze the two fatty acids from the soy-lecithin, however, the hydrolysis rate was rather inhibited by combination with the phospholipase A. Moreover, the phospholipase B activity on soy-lecithin and soy-lysolecithin was found to be increased by the presence of soy-lecithin and soy-lysolecithin in the reaction mixture, and to be inhibited by the addition of Tween 20.View full abstractDownload PDF (432K) -
Nobumaro KAWASHIMA, IKUZO URITANI1963 Volume 27 Issue 6 Pages 409-417
Published: 1963
Released on J-STAGE: February 07, 2011
JOURNAL FREE ACCESSChanges in the peroxidase activity and the patterns in sweet potato tissue infected by Ceratocystis fimbriata were investigated, by the method of starch-gel electrophoresis, DEAEcellulose column chromatography and immunochemical analysis, compared with that in cut and healthy tissue. Time-course analysis of the increment of the total peroxidase activity also showed noticeable differences between diseased and cut tissues. The peroxidases in diseased and cut tissues were composed of four major and several minor components. Though electrophoretic analysis did not show so striking differences in the patterns of peroxidases between diseased and cut tissues, there were distinct differences in the ratio of activities of major peroxidase components between both tissues. Immunochemical works indicated that peroxidase A which showed the most prominent increase in diseased tissue was found to be formed in cut tissue though the amounts were appreciably small. The activity of peroxidase C in diseased tissue was not so high as seen in cut tissue.View full abstractDownload PDF (1690K) -
Toshio YOSHIDA, Akira KOMATSU, Motoichi INDO1963 Volume 27 Issue 6 Pages 418-422
Published: 1963
Released on J-STAGE: February 07, 2011
JOURNAL FREE ACCESSThe unknown three isomers of optically inactive isopulegol, (±)-isopulegol (II), (±)-neo-isopulegol (III), and (±)-iso-isopulegol (IV) were isolated; then 3, 5-dinitrobenzoates and p-nitrobenzoates were prepared from them.View full abstractDownload PDF (597K) -
Teiichiro ITO, Tadao ISHII1963 Volume 27 Issue 6 Pages 423-426
Published: 1963
Released on J-STAGE: February 07, 2011
JOURNAL FREE ACCESS2-Acetamido-3, 4-di-O-acetyl-2, 6-dideoxy-6-S-acetyl-6-thio-D-glucopyranosyl chloride (III) was condensed with potassium thiolacetate, potassium ethylxanthate or thiourea to give three crystalline derivatives of 2-acetamido-2-deoxy-1, 6-dithio-D-glucose. An attempt to prepare 2-acetamido-1, 2, 6-trideoxy-1, 6-dimercapto-D-glucose (VII) from 2-acetamido-3, 4-di-O-acetyl-1, 2, 6-trideoxy-1, 6-di-S-acety1-1, 6-dithio-β-D-glucopyranose was described. 2-Acetamido-3, 4-di-Oacety1-1, 2, 6-trideoxy-1-mercapto-6-S-acetyl-6-thio-β-D-glucopyranose (VIII) was synthesized from the condensation product of III with thiourea.View full abstractDownload PDF (511K) -
Part VI. Spread Monolayer of the Protein Fraction of the Chicken Egg WhiteRyo NAKAMURA1963 Volume 27 Issue 6 Pages 427-432
Published: 1963
Released on J-STAGE: February 07, 2011
JOURNAL FREE ACCESSIn order to study the relation between the foaming property and the surface chemical property of the egg white proteins, each protein was spread at the A/W interface and the surface pressure of the film was measured. Simultaneously, the life time of the single bubble formed on the surface was measured. According to this experiment, it is concluded that the proteins which foam well are easily surface-denaturated and give on the surface a well-developed network consisting of the side chains of the peptide linkage.View full abstractDownload PDF (838K) -
Part I. On the Determination of Optical Purity of MentholsToshio YOSHIDA, Akira KOMATSU, Motoichi INDO1963 Volume 27 Issue 6 Pages 433-437
Published: 1963
Released on J-STAGE: February 07, 2011
JOURNAL FREE ACCESSMenthols were converted to Δ3-menthone enol acetate (VII) via menthones having only one asymmetric carbon atom. It was shown that the optical rotation of menthone enol acetate was proportional to the optical purity of starting menthols. Optical purity of original menthol could, therefore, be determined by optical rotation of menthone enol acetate derived from.View full abstractDownload PDF (584K) -
Part III. Titration of Various Starch-type PolysaccharidesTsuneo KOBAYASHI, Noboru KOBAYASHI1963 Volume 27 Issue 6 Pages 438-444
Published: 1963
Released on J-STAGE: February 07, 2011
JOURNAL FREE ACCESSAmperometric titration with iodine, a method devised by Kobayashi and Yoshida, was applied to various starch-type polysaccharides in order to elucidate the relation between the shape of titration curves and the structure of substances. Shapes of titration curves could be divided into 2 types, provisionally named the curves of amylose-type, and amylopectin-type, respectively.
Amyloses and linear dextrins with the degree of polymerization (DP) above 28 gave curves of the former type, which exhibited segments with more or less low gradient in the former half of the titration.
On the other hand, all of the branched polysaccharides, linear dextrins with DP below 25, Schardinger dextrins, and polyvinyl alcohol showed curves of the latter type, which rised linearly with added iodine.
The mode of interaction with iodine was discussed for these polysaccharides together with some observations on the starch-iodine coloration.View full abstractDownload PDF (905K) -
Part II. Two Nicotine IsomersIzuru YAMAMOTO, Hideo KAMIMURA1963 Volume 27 Issue 6 Pages 445-449
Published: 1963
Released on J-STAGE: February 07, 2011
JOURNAL FREE ACCESSSynthesis of two nicotine isomers, 5'-methylnornicotine and 6-methylnornicotine is described. The latter shows slightly insecticidal action. Myosmine type bases are easily reduced to nornicotine type with sodium borohydride.View full abstractDownload PDF (600K) -
Part III. Structure of AnabaseineHideo KAMIMURA, Izuru YAMAMOTO1963 Volume 27 Issue 6 Pages 450-453
Published: 1963
Released on J-STAGE: February 07, 2011
JOURNAL FREE ACCESSThe structure of anabaseine, which had been described as 2-(3-pyridyl)-Δ2-tetrahydropyridine, was corrected as 2-(3-pyridyl)-Δ1-tetrahydropyridine from nuclear magnetic resonance spectrum and other evidences.View full abstractDownload PDF (355K) -
Part V. Participation of Two Different Enzymes in the Decomposition of Glycol Chitin to Constituent AminosugarAkira ÔTAKARA1963 Volume 27 Issue 6 Pages 454-460
Published: 1963
Released on J-STAGE: February 07, 2011
JOURNAL FREE ACCESSIn an attempt to separate the enzyme system participating in the decomposition of glycol chitin to constituent aminosugar, the purification of chitinase of Aspergillus niger was carried out by detemining both liquefying and saccharifying activities. Using fractionation with ammonium sulfate and column chromatography by hydroxylapatite, the chitinase system of the mold was separated into different enzyme fractions, which were required for the complete hydrolysis of glycol chitin. It was found that one of these enzymes caused a rapid decrease in viscosity of glycol chitin solution, another enzyme possessed N-acetyl-β-glucosaminidase activity upon N, N'-diacetylchitobiose and β-methyl-N-acetylglucosaminide. and that glycol chitin was decomposed to constituent aminosugar by a successive action of the two different enzymes.View full abstractDownload PDF (941K) -
Part II. Identification of D-Glucosone Formed by Oxidative Browning Degradation of N-D-GlucosideHiromichi KATO1963 Volume 27 Issue 6 Pages 461-466
Published: 1963
Released on J-STAGE: February 07, 2011
JOURNAL FREE ACCESSFrom the browning degradation mixture of N-D-glucosyl-n-butylamine in methanol solution neutralized with acetic acid, D-glucosone was isolated and identified as its 2, 4-dinitrophenylosazone, in addition to the 3-deoxy-D-glucosone which had been proved as an intermediate substance of browning reaction, although the yield of D-glucosone was far less than that of 3-deoxy-D-glucosone. Under bubbling with air, the yield of D-glucosone considerably increased, indicating that its formation depended upon the oxygen dissolved. DGlucosone is regarded as an important intermediate for browning, and a mechanism for its formation from N-glucoside is proposed.View full abstractDownload PDF (751K) -
Synthesis of Theanine by the Homogenate of Tea SeedlingsKei SASAOKA, Makoto KITO, Hiroko INAGAKI1963 Volume 27 Issue 6 Pages 467-468
Published: 1963
Released on J-STAGE: February 07, 2011
JOURNAL FREE ACCESSDownload PDF (492K) -
Hideo KATAGIRI, Hideaki YAMADA, Kôji MITSUGI, Masahiro TAKAHASHI1963 Volume 27 Issue 6 Pages 469-470
Published: 1963
Released on J-STAGE: February 07, 2011
JOURNAL FREE ACCESSDownload PDF (195K)
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