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Part I. Post-mortem Changes in Adenosine Triphosphatase Activity of Myofibrils from Rabbit Muscle
Ryung YANG, Akihiro OKITANI, Masao FUJIMAKI
1970 Volume 34 Issue 12 Pages
1765-1772
Published: 1970
Released on J-STAGE: November 27, 2008
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Adenosine triphosphatase (ATPase) activity of myofibrils isolated from fresh muscle and the muscle stored at 4°C have been measured.
An increase in Mg-activated ATPase activity of myofibrils was caused by lengthened homogenization.
With the progress of aging of muscle, Mg-activated ATPase activity of myofibrils increased remarkably.
When myofibrils from pre-rigor and rigor muscle in 0.16M KCl were treated with 0.6M KCl18mM Tris-maleate solution (pH 7.0), Mg-activated ATPase activity of myofibrils at low ionic strength increased markedly. However, the Mg-activated ATPase activity of the myofibril isolated from the muscle stored at 4°C for 8 days (8-myofibril) increased slightly after the similar treatment.
The dependence of myofibrillar ATPase activity on KCl concentration became greater with the progress of aging of muscle.
These results may show that, as long as ATPase activity and the dependence of ATPase activity on KCl concentration are concerned, 8-myofibril is the most similar to the isolated actomyosin among myofibrils, although actomyosin in muscle may exist in a different form from that in solution. It is suggested that, with the progress of aging, the structural alteration of myofibril occurred and the myofibril became more susceptible to ATP-induced transformation.
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The Predominant Effect of (-)-Epigallocatechin Gallate on the Taste Nerve Response of the Toad
Muneyuki NAKAGAWA
1970 Volume 34 Issue 12 Pages
1773-1780
Published: 1970
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An astringent tea constituent, (-)-epigallocatechin gallate strongly depresses the glossopharyngeal nerve responses of the toad to other green tea constituents and presents a response pattern similar to that of green tea liquor. This substance also exhibits an inhibitory effect on responses to quinine hydrochloride and sucrose, but enhances the response to sodium chloride.
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Part I. The Oxidation of 2, 2, 5, 7, 8-Pentamethyl-6-hydroxychroman
Masao FUJIMAKI, Koichiro KANAMARU, Tadao KURATA, Osamu IGARASHI
1970 Volume 34 Issue 12 Pages
1781-1786
Published: 1970
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α-Tocopherol model compound, 2, 2, 5, 7, 8-pentamethyl-6-hydroxychroman was oxidized under oxygen bubbling. Four oxidation products of 2-(γ, γ-dimethylallyl)-3, 5, 6-trimethyl-1, 4-benzoquinone (B), 2, 2, 7, 8-tetramethyl-5-formyl-6-hydroxychroman (C), trimer (D) and tocopherylethane (G) were identified, and spirodimer (E) was tentatively identified by TLC. Two of them, (B) and (C) have not been obtained in the oxidation of α-tocopherol model compound with
p-quinone, alkaline ferricyanide and other compounds as oxidizing agent. A scheme of oxidation mechanism of α-tocopherol model compound was also proposed.
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Part V X-Ray Diffraction of α-Cyclodextrin Complexes
Ken'ichi TAKEO, Takashi KUGE
1970 Volume 34 Issue 12 Pages
1787-1794
Published: 1970
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X-Ray diffraction analysis of the α-cyclodextrin complexes with a number of organic guest molecules were carried out. Several different kinds of the X-ray diffraction patterns were obtained. It was found that different guest molecules enclosed within the void of the dextrin cause large changes in the diffraction patterns of the complexes. However, most of the diffraction patterns could be reasonably interpreted in terms of the hexagonal unit cells with minor differences in the unit cell dimensions ranging a=b=27.0_??_27.8Å and
c=14.7_??_16.7Å. The crystal structure of the complexes could be accounted for by a closest packing of channel cylinders that are made by coaxial alignments of the dextrin molecules and the cage structure in the crystal, in which the dextrin molecules align non-coaxially, may not be plausible.
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Part IV Purificaiton, Crystallization and Some Physicochemical Properties
Gen-ichi DANNO
1970 Volume 34 Issue 12 Pages
1795-1804
Published: 1970
Released on J-STAGE: November 27, 2008
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D-Glucose-isomerizing enzyme was purified in a crystalline form with a good yield
from the cells of
Bacillus coagulans, strain HN-68, and some phsicochemical properties were investigated.
The purified enzyme was homogeneous on both ultracentrifugal and disc-electrophoretical analyses. The molecular weight of the enzyme was determined to be 175, 000 and 160, 000 from the sedimentation-viscosity method and the gel filtration method, respectively.
The sedimentation coefficient (s°
20, w), partial specific volume, E
1%1cm at 280mμ, and the nitrogen content of the enzyme were determined to be 10.2×10
-13 sec., 0.705 cm
3g
-1, 10.6 and 16.2%, respectively. The integral numbers of amino acid residues per molecule cal-culated on the basis of 160, 000 were as follows; Lys
120, His
49, Arg
61, Asp
182, Thr
87, Ser
70, Glu
136, Pro
44, Gly
l06, Ala
140, Half-Cys
0, Val
53, Met
27, Ileu
51, Leu
134, Tyr
58, Phe
96, Try
l3, and amide-ammonia
80.
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Part V Comparative Study on the Three Activities of D-Glucose, D-Xylose and D-Ribose Isomerization of the Crystalline Enzyme
Gen-ichi DANNO
1970 Volume 34 Issue 12 Pages
1805-1814
Published: 1970
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Purified enzyme preparation obtained from
Bacillus coagulans, strain HN-68 requires Co
2+ for D-glucose- and D-ribose-isomerizing activities and Mn
2+ for D-xylose-isomerizing activity. The values of
Km for D-glucose, D-xylose and D-ribose were 9×10
-2, 1.1×10
-3, 7.7×10
-2M and of the relative V
max were 0.52, 1.1 and 0.25mg/min at 40°C, respectively. D-Glucose-isomerizing activity was inhibited by D-xylose and D-ribose. However, there was not a difference among three activities of the enzyme with respect to following properties: Activation energy was 14, 600cal per mol. The enzyme was inhibited in a competitive manner by tris (hydroxymethyl)aminomethane, D-xylitol, D-sorbitol and D-mannitol, and the
Ki values for these inhibitor were 3×10
-4, 2.5×10
-3, 2.9×10
-2 and 7×10
-2M, respectively. The ratio of three activities did not change by heat- and pH-treatments. Mn
2+, Co
2+ and Ni
2+ protected strongly the enzyme from heat denaturation. The enzyme can isomerize D-glucose, D-xylose and D-ribose to their corresponding ketose, but the kinetic constants and induction studies indicated that D-xylose is the natural substrate for the enzyme.
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Ikunori KOSHIYAMA
1970 Volume 34 Issue 12 Pages
1815-1820
Published: 1970
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A 7S protein isolated from soybean globulins was dissociated into a similar slowsedimenting material (subunit) by the treatment of urea and sodium dodecyl sulfate (SDS).
Sedimentation coefficients of the subunits obtained by treating with 8M urea and 0.25% SDS were 1.35S and 2.00S, and molecular weights were 22, 500 and 34, 000, respectively. These subunits by the both treatments were apparently different in conformation from the results of optical rotatory dispersion,
i.e., urea treatment caused the almost complete unfolding of the subunit structure. On the contrary, SDS treatment contributed new partial formation of_??_α-helical conformation for the subunits.
These dissociations were extremely disturbed by the presence of sodium chloride.
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Part V. The Epoxidation Products of Methyl Dehydro-β-ionylideneacetates
Takayuki ORITANI, Kyohei YAMASHITA
1970 Volume 34 Issue 12 Pages
1821-1825
Published: 1970
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Epoxidation of methyl dehydro-β-ionylideneacetates with perbenzoic acid afforded methyl 1', 2'-epoxy-dehydro-β-ionylideneacetates and then methyl 1', 2'-, 3', 4'-di-epoxy-dehydro-β-ionylideneacetates. 1', 2'-Epoxy-dehydro-β-ionone, obtained byepoxidation of dehydro-β-ionone, was treated with carbethoxymethylenetriphenlphosphorane to give ethyl 1', 2'-epoxy-dehydro-β-ionylideneacetates. Further, sensitive photooxidation of ethyl dehydro-β-ionylidenecrotonate, followed by alkaline hydrolysis, gave 1'-hydroxy-4'-keto-α-ionylidenecrotonic acid. Growth inhibitory activities of the above compounds on rice seedlings were examined.
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Sadao KATO, Tadao KURATA, Riichi ISHITSUKA
1970 Volume 34 Issue 12 Pages
1826-1832
Published: 1970
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From the pyrolysis of L-serine, ten volatile compounds including several pyrazines were identified. Pyrazines were also found from the pyrolysis of L-threonine, but not from that of alanine and considered to be characteristic pyrolysis products of β-hydroxy amino acids. At the same time, diketopiperazines, amines and carbonyl compounds were also found in addition to those described above. Formation mechanism of pyrazine compounds was also discussed.
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Part XX. Conversion of Fumaric Acid to l-Malic Acid by the Association of Two Kinds of Yeasts
Toshiro FURUKAWA, Tadaatsu NAKAHARA, Koichi YAMADA
1970 Volume 34 Issue 12 Pages
1833-1838
Published: 1970
Released on J-STAGE: November 27, 2008
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In order to convert fumaric acid to
l-malic acid continuously by hydrocarbon fermentation, the cultivation of
Candida utilis IAM 4215 or
Pichia membranaefaciens IAM 4122 which have a high fumarase activity was combined with fumaric acid fermentation by
Candida hydrocarbofumarica.
Forty strains of yeasts were tested for their fumarase activities.
P. membranaefaciens LAM 4122 and
C. utilis JAM 4215 indicated the high activity. After
C. hydrocarbofumarica was incubated in
n-paraffin medium for 5 days, fumaric acid produced was converted to
l-malic acid by the associated culture with
P. membranaefaciens IAM 4122 for 4 or 5 days. Yields of
l-malic acid based on
n-paraffin were 72% and 70% by P. membranaefaciens and
C. utilis, respectively.
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Part XIV Composition of the Triglycerides of Four Scale Insect Fats
Akira HASHIMOTO, Akio HIROTANI, Katsunori MUKAI, Shozaburo KITAOKA
1970 Volume 34 Issue 12 Pages
1839-1842
Published: 1970
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Part II. Application of NMR Spectroscopy for Configurational Analysis of (-)-Menthyl Carbinol and Related Compounds
Atushi KATO, Hiroo UEDA, Yohei HASHIMOTO
1970 Volume 34 Issue 12 Pages
1843-1847
Published: 1970
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Part V. Changes in Free Amino Acids, Ethanolamine and Two γ-Glutamyl Petides Content during the Ripening Period of Soybean
Takanori KASAI, Sadao SAKAMURA, Shuji OHASHI, Hideyuki KUMAGAI
1970 Volume 34 Issue 12 Pages
1848-1850
Published: 1970
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Kyoko SAIO, Tatsuya MATSUO, Tokuji WATANABE
1970 Volume 34 Issue 12 Pages
1851-1854
Published: 1970
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Part VI. Synthesis of 4-Amino-4-deoxy-α-D-galacturonic Acid and 4-Amino-4-deoxy-α-D-galacuronic Acid Derivatives
Takehiko NAKA, Takeshi HASHIZUME
1970 Volume 34 Issue 12 Pages
1855-1858
Published: 1970
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Makoto TAJIMA, Makio MORITA, Masao FUJIMAKI
1970 Volume 34 Issue 12 Pages
1859-1861
Published: 1970
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Kanji OHYAMA, Tohru KOMANO, Konoshin ONODERA
1970 Volume 34 Issue 12 Pages
1862-1866
Published: 1970
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Naomichi ISO, Daijiro YAMAMOTO
1970 Volume 34 Issue 12 Pages
1867-1869
Published: 1970
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Koichi OGATA, Yoshikazu IZUMI, Yoshiki TANI
1970 Volume 34 Issue 12 Pages
1870-1871
Published: 1970
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Koichi OGATA, Yoshikazu IZUMI, Yoshiki TANI
1970 Volume 34 Issue 12 Pages
1872-1874
Published: 1970
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Yoshio NAKAO, Masakazu KIKUCHI, Masaru SUZUKI, Muneharu DOI
1970 Volume 34 Issue 12 Pages
1875-1876
Published: 1970
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1970 Volume 34 Issue 12 Pages
e3a
Published: 1970
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1970 Volume 34 Issue 12 Pages
e3b
Published: 1970
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