The results of sedimentation studies revealed that “myosin B” extracted from rigor muscle was different from myosin B from fresh muscle; the former contained less contami-nating myosin A and less main component but more heavy component than the latter did. The proportion of constitutional myosin A to actin in the former was less than that in the latter.
“Myosin B” from post-rigor muscle was very similar to that from rigor muscle in the sedimentation behaviours.
The extrapolated sedimentation coefficient of the main component in myosin B from fresh muscle was 99S.

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Characterization of the theanine synthesizing enzyme found in tea seedlings was carried out. Evidences suggest that this enzyme seems to be a synthetase peculiar to the tea plant, having a high affinity for ethylamine.

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Four kinds of substitute milk containing spray-dried skimmilk were fed to eight Holstein male calves of about 1 wk. of age for 4 wk. In the skimmilk diet the growth rate and feed efficiency were the lowest. The addition of 4% crude soybean lecithin to the skimmilk diet was found to increase the growth rate and feed efficiency to a remark-able degree.
The apparent digestion coefficients of tallow were about 88% throughout three diges-tion periods and no age effect upon the digestibility was observed, whereas the digestibility of wheat starch showed a tendency to increase in accordance with the advanced age. The calves could not digest the starch to an appreciable extent for about 1 wk. after the starch-containing diet was offered. After a lag of about 1 or 2 wk. the adaptive mechanism to digest starch increased rapidly.

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1) As nitrogen source, urea is more effective than ammonium nitrate.
2) The C/N ratio 30 in most suitable within the limits of this experiment.
3) The addition of corn steep liquor can shorten the time of lag phase.
4) Y-3 strain cannot assimilate the hydreo-carbons of aliphatic series lower than C₉.
5) Percentages of conversion from hydro-cabon (calculated from optical density by Fig. 7) into cell substance (dried cells) are as follows;
n-decane 56.4% n-undecane 43.6%
n-dodecane 43.4% n-tridecane 47.5%

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We found that the yeast Y-3 strain reported in the previous paper¹⁾ has a diterminal oxidation system of hydrocarbon.
This yeast capable of growing in mineral-salts solution with hydrocarbons as sole source of carbon produced a series of dioic acid from n-undecane. These acids are 1, 11-undecane dioic acid, 1, 9-nonane dioic acid (azelaic acid), 1, 7-heptane dioic acid (pimelic acid) and 1, 5-pentane dioic acid (glutaric acid). 1, 10-Decane dioic acid (sebacic acid) was also isolated from n-decane cultures.
Azelaic acid was partially transformed into pimelic acid and glutaric acid by treating it with resting cells of this yeast.
1, 11-Undecane dioic was also transformed into azelaic acid pimelic acid, and glutaric acid by the same treatment as described above.

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The neutral fraction of the essential oils from three kinds of black tea (same samples as described in the previous paper, ¹⁾ i.e., Assam, Shan and Benihomare) was separated into carbonyl and carbonyl-free fractions and analysed by gas chromatography. On the basis of relative retentions and aroma of effluents with the references of the chromato-graphic data obtained by the previous works, ^2_??_4) the major alcohols were found to be cis-2-pentenol, n-hexanol, cis-3-hexen-l-ol, three isomers of linalooloxide, linalool, nerol, geraniol, benzylalcohol, and phenylethylalcohol. In the carbonyl fraction, phenylacetaldehyde was newly identified, and besides it, the presence of iso-and n-butyr-, iso-and n-valer-aldehyde, methylethylketone, trans-2-hexenal, benzaldehyde were recognized. There were no differences in the components among three kinds of black tea, but the relative quantity of each component in the essential oil was different among three varieties.

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A marked rise in the phenylalanine ammonia-lyase activity and the polyphenol synthesis was observed in sliced roots of a sweet potato. The enzyme activity was found to be localized in the root tissue adjacent to the sliced surface. In this region, the synthesis of polyphenols was much higher compared to the inner tissues. When the specific inhibitors for the protein and nucleic acid biosynthesis such as an actinomycin D and blasticidin S were added to the tissues by vacuum infiltration technique, both the development of phenylalanine ammonia-lyase activity and the synthesis of polyphenols were severely prevented. These results suggest the important role of phenylalanine ammonia-lyase in the polyphenol synthesis and de novo synthesis of the enzyme protein molecule in the sliced tissues.

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The H-, Na- and Ca-forms of succinoglucan 1OC3 form homogenous dispersions in water. The viscosities of 1% aqueous dispersion of the H- and Ca-forms were 1560 and 2500 centipoises respectively at 30°C. The viscosity of the Na-form, which is extremely low, was increased greatly by an addition of many inorganic salts and especially the Fes+ and Al³⁺ iones. The viscosity of the Ca-form did not change by the presence of sodium chloride or calcium chloride.
The viscosities of Ca-form, and Na-form in the presence of 1% sodium chloride became near zero at 65.5°C and 69.5°C, respectively. When the dispersions were kept at 70°C or 90°C for ten minutes, the viscosities of the H-form, the Na-form, and the Ca-form in the presence of sodium chloride increased greatly. The viscosity of the Ca-form and that of the Na-form in the presence of 1% sodium chloride were essentially indepen-dent of pH.

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Various conditions of the autolysis of Asp. oryzae were tested systematically and the fol-lowing results were obtained:
(1) Below 45°C, autolysis occurs faster at higher temperatures. It takes 1 day at 45°C and 3 days at 30°C for the autolysis to be completed.
(2) The optimum pH of autolysis is 5 as for the excretion of amino acids and nucleic acids.
(3) At the optimum conditions, 90_??_100% of nucleic acids, 75% of amino acids and 20% of sugars in the mycelia are excreted into medium.
(4) The autolysis occurs readily when the mycelia were incubated in water without glucose in anaerobic condition. And, the autolysis is inhibited by the addition of 2% of glucose under both aerobic and anaerobic conditions.
(5) The autolysis is stimulated by lipophilic compounds such as toluene and sodium salts of fatty acids. Hydrophilic groups have no stimulatory effect.

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Aspergillus oryzae was autolyzed at pH 5.4 at 30°C. The morphological changes during autolysis were observed with a phase-contrast microscope, and the degradation products of proteins, nucleic acids, and sugars were analyzed.
1) The cytoplasmic materials disappeared during three days of autolysis, and the cell wall remained after autolysis. Under an aerobic condition, mycelia lived for a while consuming the reserve materials, and vacuoles were formed in the midst of the cell, becoming larger till they occupied the whole space of the cell. Under an anaerobic condition, the cytoplasmic materials began to dissolve at the marginal site contacting with the cell wall, and the whole cell became empty leaving a few particles.
2) Seventy-five per cent of proteins were excreted into medium during autolysis, 60% as free amino acids, 15% as peptides. Protein itself was hardly present in the autolyzate.
3) Glucose, ribose and three other sugars were detected in the autolyzate, and glucosamine was also detected in the hydrolyzate of the autolyzate.
4) Eighty-one per cent of the cellular nucleic acids was excreted into medium as uridine (28%), xanthine (24%), and hypoxanthine (17%). Nucleotides were hardly present in the autolyzate.

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Inosine-5', 2'-diphosphate was found to be synthesized by incubating 2'-IMP and p-nitrophenylphosphate with the bacteria char-acterized to phosphorylate at C₅', while the others capable of synthesizing 3'(& 2')-nucleotide were found to synthesize isomeric 5', 3'- and 5', 2'-diphosphate of inosine from 5'-IMP and the same phosphate donor. Analogous phosphorylations of the other
nucleotides were observed to be carried out under the same relationship as that of IMP.

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