The division of mitochnodrial nuclei in
Amoeba proteus,
Paramecium caudatum,
Nitella flexilis and
Allium cepa was investigated using 4'-6-diamidino-2-phenylindole (DAPI) epifluorescent microscopy. The mitochondria each contained one mt-nucleus in
Amoeba proteus and
Paramecium caudatum and one or two mt-nuclei in
Nitella flexilis and
Allium cepa. The ratio of fluorescent intensity (grain density of negative film) among one DNA molecule of a T4 phage particle and mt-nuclei of spherical mitochondria in
Physarum polycepaalum,
Amoeba proteus,
Paramecium caudatum,
Nitella flexilis and
Allium cepa was about 1:16:22:5:0.4:0.4. That is, the mitochondrial DNA content in
Nitella flexilis and
Allium cepa was much smaller than those in Amoeba proteus and Paramecium caudatum. The mitochondria and their nuclei in
Amoeba proteus and
Paramecium caudatum as well as
Physarum polycephalum divided simultaneously by constriction, while segregation of mitochondria) nuclei occurred before the mitochondrial division in
Nitella flexilis and
Allium cepa. The fluorescent intensity of mt-nuclei in dumbbell-shaped mitochondria of
Paramecium caudatum and
Allium cepa was about twice that in spherical mitochondria. Therefore, mitochondrial DNA probably duplicated and divided equally into daughter mitochondria.
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