An acrotrisomic plant was isolated in the progeny of the primary trisomic for chromosome 4. It was designated as acrotrisomic 4L
4S. Five marker stocks were crossed as male to the acrotrisomic 4L
4S. The F
2 segregation in the trisomic tests showed that the gene
i, previously located in the distal part of the long arm, segregated in a disomic ratio, indicating that
i is located on the missing segment of the long arm. The genes
lk5,
gs1 and
yh in the short arm also segregated in a disomic ratio, indicating their locations should be in the deficient part of the short arm of chromosome 4. The gene
f10 on the short arm showed a trisomic ratio, indicating that this gene is located on the remaining proximal segment of the short arm. Acrosomic trisomic analysis provide, a valuable approach for physical localization of genes on barley chromosomes.
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