In this report the author has enumerated the differences in interpretations of the mitosis studied both in fixed preparations and in
in vivo observations. The author especially maintains the opinion that any movement of chromosomes in mitotic cells should be reinvestigated thoroughly by
in vivo observations in the same way as studies on any movement of intracellular organelles, e.g. plastids, mitochondria, rotation of nucleus, streaming of protoplasm, etc.
1. Practically all behavior of mitotic cells described in cytology books has scarcely made reference to the genetic back-ground of the mitosis.
2. The breakdown of nuclear membrane before spindle formation in higher plants and animals is an impossibility. The nuclear membrane in eukaryotes is predicted and controlled by genes and genetic information which exist as long as the cell lives.
3. The participation of centrioles in the formation of spindle fibers and in the chromosome movement in anaphase is also a misinterpretation due to a disregard of genes and genetic information under which centrioles behave as primordia of organelles for cell movement.
4. In
in vivo observations, the continuous movement of chromosomes in anaphase can be seen objectively in a three dimensional space of living spindles, whereas the same behavior of chromosomes in fixation cytology is made up by animating a series of chromosome images in sectioned plane mitotic cells in fixed preparations under observers' subjective influences.
5. The teleonomy and the cause and effect relationship among the behavior of mitotic cells, viz. reversible transformation of nuclear sap proteins, the change of nuclear contour from sphere to spindle-shape, the development of spindle background fibrils, the movement of chromosomes in anaphase by shortening of kinetochore fibers, formation of daughter nuclei, the accumulation of ATP at spindle poles, etc. are practically disregarded of the descriptions on the mitosis in current cytology books.
抄録全体を表示