For the Incentive Award of the Mass Spectrometry Society of Japan Incentive Award, this review will over view the works of mass spectrometry from Setou lab. Mainly, papers 2002–2010 are about development of imaging mass spectrometry. Papers 2010–2015 are mainly the application of imaging mass spectrometry for clinical samples. Recently, several papers are about the applications of secondary ion mass spectrometry. Most significant findings are that various lipid species have their own tissue distribution pattern in tissues and cells through these studies.