Journal of the Mass Spectrometry Society of Japan
Online ISSN : 1880-4225
Print ISSN : 1340-8097
ISSN-L : 1340-8097
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Volume 57, Issue 2
Displaying 1-14 of 14 articles from this issue
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MEMORIAL WRITINGS
REGULAR PAPERS
  • Study of Cuticular Chemicals of Formica japonica Ant Using Gas Chromatography/Mass Spectrometry
    —Temporal Modulation in the Pattern of Cuticular Hydrocarbons—
    Keisuke ISHII, Makiko ASAHARA, Mitsuo TAKAYAMA
    2009 Volume 57 Issue 2 Pages 61-70
    Published: April 01, 2009
    Released on J-STAGE: April 15, 2009
    DOIhttps://doi.org/10.5702/massspec.57.61
    JOURNAL FREE ACCESS
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    Change in cuticular hydrocarbons (CHCs) and cuticular hydrocarbons profile (CHP) of the Formica japonica ant collected over a three-year period comprising 2006 (May to October), 2007 (May to October), and 2008 (March to October) were examined by gas chromatography/mass spectrometry (GC/MS) and a principal component analysis method. It was found that cyclic changes in CHPs for n-monoalkenes and branched monomethyl alkanes occur every year. This indicates that the observed tendencies include n-monoalkenes undergoing a relative increase from March or May to June, and a decrease from July to October, whereas branched monomethyl alkanes undergoing a relative decrease during March or May to June, and increase during July to October.
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  • Optical Resolution of 1,2-Diamino Compounds Using Advanced Marfey's Method
    Yuriko NOZAWA, Ken-ichi HARADA, Akemi RYODA, Takuya KUMAMOTO, Waka NAK ...
    2009 Volume 57 Issue 2 Pages 71-74
    Published: April 01, 2009
    Released on J-STAGE: April 15, 2009
    DOIhttps://doi.org/10.5702/massspec.57.71
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    Advanced Marfey's method was successfully applied to the optical resolution of 1,2-diaminoethane compounds. The elution behavior of 1,2-diphenylethylene-1,2-diamine and 1,2-bis(2-methylphenyl)ethylene-1,2-diamine derivatized with L- and D-FDLA was carefully investigated and the following regularity was found: 1) The (S,S)-isomers are eluted prior to the (R,R)-isomer; 2) The (R,R)- and meso-isomers are closely eluted; 3) There is no difference between the retention time of both the L- and D-derivatives of the meso-isomer; 4) This regularity is fundamentally consistent with that of diamino acids such as diaminopimelic acid and diaminosuccinic acid. Finally, it was confirmed that this method along with the DL-FDLA method could be applicable for the determination of the absolute configuration of 1,2-diaminoethane compounds because the method can definitely determine it by the elution order.
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  • Microdetermination of Catechol Estrogens by Liquid Chromatography-Electrospray Ionization Tandem Mass Spectrometry Combined with Picolinyl Derivatization
    Kouwa YAMASHITA, Takanori KAWAHATA, Madoka TAKAHASHI, Mitsuteru NUMAZA ...
    2009 Volume 57 Issue 2 Pages 75-80
    Published: April 01, 2009
    Released on J-STAGE: April 15, 2009
    DOIhttps://doi.org/10.5702/massspec.57.75
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    A simultaneous method for quantification of four catechol estrogens, namely, 2-hydroxyestrone, 2-hydroxyestradiol, 4-hydroxyestrone, and 4-hydroxyestradiol by liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) was developed in a positive mode for analyzing a simple picolinyl derivatization. Reaction of these estrogens with picolinyl derivatization reagent resulted in the formation of di- or tri-picolinyl derivatives, and the derivatives exhibited single charged protonated molecules as base peaks in a positive mode in electrospray ionization/mass spectrometry (ESI-MS). Picolinyl derivatives of these catechol estrogens provided approximately 10 times greater ESI responses in LC-ESI-MS/MS (selected reaction monitoring) compared with those of underivatized molecules in a negative mode. The use of picolinyl derivatizatives, solid-phase extraction, and a 13C-labeled internal standard helped us in determining these catechol estrogens whose quantification limit was 20 pg for every sample. The present method was applied for quantifying these catechol estrogens in a biological sample.
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  • Determination of Dynamic Ranges for Quantitative Analysis Using Electrospray Droplet Impact Ionization and Matrix-Assisted Laser Desorption Ionization
    Daiki ASAKAWA, Yutaka HASHIMOTO, Kenzo HIRAOKA
    2009 Volume 57 Issue 2 Pages 81-87
    Published: April 01, 2009
    Released on J-STAGE: April 15, 2009
    DOIhttps://doi.org/10.5702/massspec.57.81
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    The dynamic ranges for quantitative analyses of etoposides using a desorption/ionization technique, electrospray droplet impact, and matrix-assisted laser desorption/ionization were investigated. Etoposide and ethyl-etoposide were used for analyte and internal standard, respectively. The calibration curves show a proportional relationship in the range of ∼4 orders of magnitude, and the coefficients of determination (R2) well exceed 0.9.
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  • Differentiation of Coelenteramide Sulfates from Phosphates by Hydrogen/Deuterium Exchange Coupled with Ion Trap Mass Spectrometry
    Issei DOI, Masaki KUSE, Yosuke NAKASHIMA, Naoki TANI, Minoru ISOBE
    2009 Volume 57 Issue 2 Pages 89-95
    Published: April 01, 2009
    Released on J-STAGE: April 15, 2009
    DOIhttps://doi.org/10.5702/massspec.57.89
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    In general, it is difficult to distinguish between sulfate and phosphate groups in natural products, because these groups are found only in trace amounts in natural products. We have devised a hydrogen/deuterium (H/D) exchange method using the liquid chromatography mass spectrometry technique for establishing a general methodology to distinguish between sulfated and phosphorylated compounds. Sulfate- or phosphate-containing compounds were differentiated by the H/D exchange method based on the number of exchangeable hydrogen in deuterated sulfate (-SO3D) and phosphate (-PO3D2), respectively. In this paper, we describe an application of this method to a natural product and in particular to Watasenia oxyluciferin that is found in the firefly squids (Hotaru-ika in Japanese) for the purpose of identifying coelenteramide disulfate.
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  • Determination of Acetone in Human Breath by Lithium Ion Attachment Mass Spectrometry
    Yoko NUNOME, Kozo MATSUMOTO, Kuniyuki KITAGAWA
    2009 Volume 57 Issue 2 Pages 97-101
    Published: April 01, 2009
    Released on J-STAGE: April 15, 2009
    DOIhttps://doi.org/10.5702/massspec.57.97
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    Lithium (Li) ion attachment mass spectrometry (IAMS) is a highly sensitive method for detecting volatile organic compounds (VOCs) without fragmentation in real-time. We have therefore developed a novel IAMS system for direct determination of trace amounts of VOCs by modifying the sampling cone interface. This technique was successfully applied to in situ analysis of human breath. It was shown that the concentrations of acetone in 7 healthy subjects ranged from 0 (not detectable) to 28.5 ppbv (parts per billion by volume) after a 15-hour overnight fast. The detection limit (3σ) is 0.55 ppbv for acetone and the relative standard deviation (RSD) is less than 9.7%. These results demonstrate that the newly developed IAMS system provides simple, rapid, and sensitive measurements of trace amounts of acetone in human breath, and that the system is promising for early diagnosis of diabetes.
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NOTE
TECHNICAL REPORT
  • Optimization of a Home-Built Desorption Electrospray Ionization Source and Its Application in Rapid Analysis
    Arifumi YOSHIMOTO, Shoichi MOTOKI, Yusuke MORIWAKI, Hideya KAWASAKI, R ...
    2009 Volume 57 Issue 2 Pages 109-115
    Published: April 01, 2009
    Released on J-STAGE: April 15, 2009
    DOIhttps://doi.org/10.5702/massspec.57.109
    JOURNAL FREE ACCESS
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    High-performance liquid chromatography/mass spectrometry (LC/MS) is the most useful tool for qualitative and quantitative chemical analysis in the life sciences. However, sample preparation and LC separation demand an extraordinary amount of time and effort. Desorption electrospray ionization (DESI) and direct analysis in real time (DART) techniques that obtain mass spectra of samples without difficulty have been developed in recent years. We connected a home-built DESI ion source to an existing mass spectrometer and examined its performance. In this paper, we report the application of DESI to thin-layer chromatography (TLC)/DESI-MS in rapid analysis of medicines in serum and concentrated analysis with a solid-phase extraction disk.
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