We could first discover that nephritis patients excreted a large amount of nucleic acid in their urine. By a modified Hammarsten method, we extracted the nucleic acid from the nephritis urine, which was identified by the following characterizations: the maximum absorption at 260mu, the minimum at 230mu, phosphate content 8.7%, RNA/DNA ratio 1.6, diphenylamine and orcine reaction positive, and such five bases released on hydrolysis as adenine, guanine, cytosine, thymine and uracil. We detamined colorimetlycaly this urine nucleic acid amount by the use of diphenylamine reaction in the modified Schneider fraction. This nucleic acid appears to be derived from the destroyed renal cells, which was demonstrated to be connected closely with proteine, cylinders and renal epithelium excreted into the nephritis urine. Nucleic acid was not excreted in the urine of a healthy person, nor in the urine of cystitis and urethritis. The excretion of nucleic acid is specific to nephritis and in-creases in proportion to the disease.
In 1938, Bock and Frenzel succeeded in produceing pancytopenia by the ligation of the splenic vein in rabbits. The results of the experiment was interpreted as supporting evidence of the existence of splenic hormones with an inhibitory influence on hematopoiesis. It was believed that the ligation of the splenic vein, blocking the splenic blood from the liver, resulted in the splenic hormones reaching the bone marrow in a higher concentration than normal, and that liver was capable of attenuating or otherwise an affecting these hormones. Jombres and Nielsen carried out the similar experiment with simultaneous bone marrow studies and Jombres came to the view that the pancytopenia occured as a result of an inhibited emission of the cells from the marrow with an accumlation of immature cell types, and Nielsen supposed the changes in the peripheral blood due to an incresed destruction of blood cells in the congested spleen because of no changes in the bone marrow. In the following we should give a brief accounts of the results of some experiments in dogs, in which we have repeated Bock and Frenzel's procedure with or without splenectomy following the ligation of the splenic vein. 1) The ligation of the splenic vein blocking venous return of the spleen to the liver resulted in normochromic and normocytic anemia with slight reticulocytopenia and initially moderate depressed erythropoiesis in bone marrow. 2) The congestion of the spleen improved quickly after the ligation of the splenic vein and the collateral communications of splenic vein to portal vessel were accomplished as early in the 2nd postoperative week. 3) The splenectomy after 4, 7, 14, and 28 postoperative days did not give remarkable influences on the course of the anemia. It is suggested that the anemia produced by the ligation of the splenic vein is due to the initial and temporal depression of the erythropoiesis and that the rôle of the spleen on this anemia must not be evaluated so excessively and the data obtained give not definite information on the pathogenesis of the anemia.
Though the studies on the relation between pituitary-adrenal hormones and reticuloendothelial system have been reported by several authors, their results do not all agree. The author intended with the present study to determine the influence of these hormones on the function of R. E. S. to manage the hen erythrocytes intravenously injected in rabbits with Oh's method, which indicates the activity of R. E. S. with the endurance of remaining of nuclei in peripheral blood of rabbits after injection of certain quantity of hen erythrocytes. The hormones administered were a single injection of 2.5mg per kg of DOCA, 12.5mg per kg of cortisone intramusculary, 1.5mg per kg of ACTH intravenously, 0.15mg per kg of adrenalin subcutaneously. The results are as follows: 1) In animals examined from 12 hours to one week after the injection of DOCA, the nuclei disappeared from the peripheral blood earlier, that is, within 2 hours, than those in normal. This means that R. E. S. function is accelerated by DOCA. 2) On the contrary, the function of R. E. S. of animals examined 6 to 12 hours after the injection of Cortisone was apparently depressed. The nuclei remained over 3 hours in peripheral blood. 3) In 9 of 11 animals injected with ascorbic acid direct after DOCA, the endurance of the remaining time of nuclei in peripheral blood was normal. It is considered that ascorbic acid inhibits the accelerating effect of DOCA on the R. E. S.. 4) R. E. S. was accelerated 2 to 12 hours after injection of ACTH, but normal after 24 hours. 5) When DOCA and cortisone were injected in the same time in rabbits, the influence of cortisone appeared earlier than DOCA and R. E. S. were depressed till 6 to 12 hours after injection, but after 24 hours the effect of DOCA became dominant and R. E. S. were accelerated. It is considered that these difference of the influence of both hormones is introduced by the difference of the velocity of their resorption and exceretion. 6) After the administration of adrenalin no influence on the R. E. S. was seen. 7) The inhibiting or accelerating effects of those hormones were not observed in the splenectomized animals. The spleen seems to play an essential rôle to manage the heterogenous blood cells in the animals.
The examination was carried out to study the influence of hypoxemic stress on liver function in high altitude in 26 healthy subjects who, climbing Mt. Norikura, 2740 metres above the sea-level, on a 4 hours bus-trip, stayed there for several days. The standard laboratory tests used for test of liver function were estimation of serum bilirubin, urinary urobilinogen, BSP and Azorubin S test, hippuric acid synthesis, flocculation tests (CCFT, TTT, Kunkel' T and Phenol test) and serum protein proportion, A/G ratio by Biuret and electrophoresis. Subjective complaints were also observed during the period. The results are as follows: 1) Some disturbances of liver functions were perceived in many cases, especially by the tests such as BSP and Azorubin S, urinary urobilinogen excretion, and HAS and CCFT, for the first week after their climbing, and the results obtained, however, were not strictly parallel in every case. The disorder of these partial liver functions recovered itself to the normal level on the tenth day as a whole. 2) The other laboratory tests remained normal. 3) Similarity was found out between subjective complaints of the subjects tested in high altitude and those of potential liver-diseased patients. The time for such subjective symptoms to reveal themselves was in complete agreement with the time when the liver was slightly damaged as stated in the above-mentioned (1). 4) The climbing to high altitude, that is, hypoxemic stress did the liver-diseased patients much more harm to their livers than the healthy subject. 5) BSP and urobilinogen excretion in high altitude, together with circulating eosinophils and urinary 17-ketosteroid excretion, showed similar changes, it is inferred, with that of ACTH therapy to patients without any liver disorder.
We have reported a case of extramedullary intradural tumor on middle cervical region which showed no radicular symptom. Subsequent operation revealed a neurilemmoma, located longitudinary from left cranial dorsal to right caudal ventral. These observations might lead to the great possibility of stimulation to posterior horn, but, to our great interest, the patient had no sign and symptom of the corresponding radicular irritations.