The content of fluorogenic corticoids in plasma was estimated in asthma bronchiale by two different methods, of Oda and of Sweat. By the former method the decrease of fluorogenic corticoids in plasma of patients was not significant, but it has been proved by the latter method that the so-called compound F-like substances were greatly decreased, especially in the attack. After the investigations of Oda and Takeda Sweat method is more reliable than the others in relating to the amount of compound F at least in the plasma of human beings. The content of the compound B-like substances in plasma was variable thereby.
We have had recently three cases of the chronic type of idiopathic thrombocytopenic purpura. All of the cases showed remarkable increase of platelets by splenectomy. The genesis of this disease researched, the existence of platelet reducing factor was found in patients sera and extracts of the spleen. And yet it was made sure through bone marrow streaming examination for this factor to act directly upon the bone marrow. This factor transfered into the cerebrospinal liquor and was secreted in the urine. Moreover, the function (movement and platlet production) of the Megakaryocyte was observed by tissue culture of patients bone marrow and taken microcinematograms in success. The number of the megakaryocytes decreased in two cases and increased in one case of the three compared with normal. Most of them had neither motility nor tentacle-like filament in spite of their maturity, and thus showed a severe hypofunction. The number of the megakaryocytes after splenectomy in all cases recovered to the normal. They stretched out many strange and complicated pseudopodia in all direction, and showed vigorous migration as well as striking formation of tentacle-like filament. A phenomenon of platlet separation was sometimes observed at the top of their filament. From above-mentioned findings, the followings could be considered; The splenectomy on the patients with chronic idiopathic thrombocytopenic purpura resulted in disappearance of the splenogenic toxic substance and immediate recovery of both function and number of the megakaryocytes by elimination of the disturbance of their development and production. It is concluded that the genesis of chronic idiopathic thrombocytopenic purpura mainly exists in the hypofunction of the megakaryocytes.
In the previous reports, I dealt with the influence of the cancerous gastric juice on the liver catalase and also on the phosphatase. The liver arginase is the subject of the present paper. The results obtained were as follows; 1. The liver arginase activity of normal rats ranged from 24.3 to 38.4, and the average value was 31.2. 2. The activity of the enzyme was depressed by injection of the cancerous gastric juice, indicating 17.5-25.5, average 21.4. 3. No remarkable change of arginase activity was observed when injected non cancerous gastric juice as control experiment. 4. The precipitate of cancerous gastric juice by trichloracetic acid proved arginase depressing effect, but the supernatant fluid had no effect on the enzyme.
29 cases of aplastic anemia admitted to our clinic were observed mainly from the viewpoint of prognosis and curative effect and the following results were obtained: Age, hemorrhagic diathesis, blood pictures and bone marrow pictures were believed to be likely a good indicator to determine prognosis: general speaking, bad prognosis was found relatively in high frequency either in older patients over 60 years or in younger ones under 20. Gingival bleeding and petechiae in the skin were observed in many cases, but bloody stool, genital bleeding, meningeal hemorrhage, large epistaxis and retinal bleeding were found only in cases with bad prognosis. A prolongation of bleeding time or clotting time and a decreased blood capillary resistance were found in either cases. It was revealed that after treatment the prolongation of bleeding time was markedly reduced in many cases with good prognosis, but on the contrary, in cases with bad prognosis the prolongation of the time was found to be much increased. In blood pictures R.B.C. count under 1.5 millions per cmm, blood platelet count under 30, 000 per cmm or W.B.C. count under 3, 000 per cmm was observed considerably in large number of cases. In the differential count decreased neutrophils under 40%, increased lymphocytes over 50%, decreased monocytes under 3% or aeosinophils was observed frequently in cases with bad prognosis, while not so frequently in those with good prognosis. It was difficult to determine prognosis by means of the nucleal cell count of sternal punctate. The decrease of either nucleated red cell under 10% or granulocyte under 40% in the differential count of myelogram was found in many cases with bad prognosis. Repeated blood transfusion (inclusive of intrasternal transfusion) and therapeutics such as folic acid (especially in large dose), vitamin B12 or methionine were considered to be effective and above all, the combination therapy of blood transfusion and folic acid was the most effective. Consequently it was believed that early diagnosis and early treatment are the most important and also that this disease needs especially improvement of blood disorders as well as treatment of hemorrhagic diathesis.