Mice were used for the study. The liver was examined microscopically without severance from the body (transillumination method) following the injection of a hepatotonica or a choleretica into the caudal vein. The following conclusions were made as based on the observations. 1. Calcium paraoxybenzoate, sodium paraoxybenzoate and sodium hippurate increased the blood stream velocity and the blood flow. Calcium paraoxybenzoate seemed more effective in this respect than the sodium salt. 2. Sodium dehydrocholate increased the velocity, but the increase of the blood volume was not marked. Intercellular spaces of the liver were dilated considerably and the bile production was augmented by this compound. It appeared that the stegnation of the bile occurred temporarily in the intercellular space due to choleresis. 3. No. demonstrable change was induced by the administration of sodium thiosulfate. 4. ACTH was most effective in accelerating the blood stream. Some evidence was obtained that ACTH renders the damage of cells by carbon tetrachloride less severe and restores the normal blood flow quickly. 5. After the injection of dye-stuffs such as azorbin S, bromsulfalein, phenolsulfonph-thalein, scarlet red and fluorescein, the color was recognized in the blood stream. The interceliular spaces were seen dilated and the pigment granules appeared therein. 6. If the injection of a dye was followed by the administration of a choleretica or a hepatotonica, the pigment granules disappeared more rapidly than in those receiving only a dye. Sodium thiosulfate was without effect in accelerating the dye excretion.
I took up the problem of the P.B.V. estimation initiated by Stewart-Ebert, with special reference to the criticism on the method, investigating what does the measured P.B.V. mean. 1. T-1824 was injected into pulmonary artery, then the mean pulmonary circulation time was determined. Being put both this value and datum of output estimated by Fick-Cournand's method into Stewart's formula, the P.B.V. was calculated. 2. While in health the mean P.C.T. was on the average 11.3±1.2 sec. and the P.B.V. 1147±238cc, in essential hypertension without congestive heart failure (8 cases) the former was on the average 19.4±1.4 sec. and the latter 1717±370 cc. 3. There are also mentioned the results obtained in bronchial asthma, pulmonary emphysema, ligation of pulmonary arterial stem, mitral stenosis, mitral insufficiency and congenital cardiac anomalies. At the same time the blocd flow through bronchial artery and bronchopulmonary vascular shunt were examined. 4. Fluctuation of P.B.V. and other circulatory dynamical data were observed in 4 patients suffered from essential hypertension before and after the application of depressant drugs and in 2 patients with bronchial asthma before and after the use of aminophylline. 5. The three components of P.B.V. decomposed into O2-consumption, A-V difference and mean P.C.T. were estimated respectively and the reje ted ellipse area was set up in both health and essential hypertensive group without complication. As a rule data measured in essential hypertension exsist out of the ellipse area found in health. When data obtained in the certain hypertensive cases are put into the equation as to essential hypertension, then there may not only be clarified the correlation between each factor, but also be able to predict whether cardio-pulmonary complication may exsist or not. 6. P.B.V. calculated from Stewart's method i) does not contain the blood volume congesting in lungs, however, ii) does contain the blood volume circulating from left atrium to femoral artery. Increased P.B.V. found in arterial hypertension or left ventricular failure is, at least in part, due to the increased blood volume in left cardiac hypertrophy and dilatation. At this juncture it is noteworthy that the change of intracardiac chamber volume is quite of different manner depending upon whether the hypertrophy is concentric or eccentric. However, by means of routine fluoroscopy including angiocardiography, it cannot closely be investigated to what extent does the blood volume in left cardiac chamber partake with P.B.V., provided that an approximate calculation of the blood volume in aorta can be made by Wezler-Böger's method.
Some modifications upon Wezler's new method which is published in 1949 were made by us, as follows; 1. To registrate the pulse wave a beryllium membrane was applied and the mechanical movement was amplified electrically. 2. Xray-kymographically the cross section of aorta was estimated and corrected by the height of blood pressure level. 3. The correlation between manschette pressure and intraarterial pressure which are put into Broemser-Ranke's formula was examined closely. There are taken place the comparative studies upon both Wezler-Böger's and Fick-Cournand's method. i) According to W-B's method, in 20 healthy men there were determined M. V., W and E'; on average, 6.04±1.22l/min., 1327±231dyne cm-5 and 1640±295dyne cm-5 respectively. ii) M. V. of 16 patients with bronchial asthma is almost within normal range, while E' is somewhat increased. iii) 128 cases suffering from essential hypertension were classified, under consideration of years, according to both W-B's and K-W's methods. iv) 7 of 10 patients with congestive heart failure showed subnormal M. V., augmented W and E', while in 3 among them there could be found changes with reversal direction. v) Hemodynamical values during each cardiac cycle in arrhythmia were determined; typical case records were explained. vi) In 23 cases M. V. determined by W-B's method is on average by 0.2l/min higher than that estimated by F-C's. vii) Before and after the measurement by F-C's method, W-B's method was applied and from hemodynamical view point it was investigated to what extent does F-C's method influense upon psychosomatic state of patients. viii) Concerning to each case which reveals remarkable difference between W-B's values and F-C's, the possible causes were discussed. F-C's values show the averaged data during venesection, whereas W-B's values those estimated during only one cardiac cycle. F-C's values depends mainly upon the state of venous side while W-B's values chiefly upon that of arterial side. In consequence in the case of arrhythmia and congenital anomalies there are assumed to develop striking difference between two kinds of values estimated by F-C's method and W-B's. ix) It is a common defect of modern technique, not to be able to undertake F-C or W-B at the cardiopulmonary emergency. The maniplation of W-B's method can be performed simply and unbleedingly, sothat to the contrary of F-C's method, W-B's can be repeated in the same individual at every request. But W-B's method has of course the limitation of its applicating. Thus there is recognized the availability either in W-B's method or F-C's and. vice versa. Consequently in order to study the hemodynamics, it may be more ideal that both methods can be adopted at the same time but if there is a circumstance under which one of them can only be applied, it is hoped that the superiority of two methods must be utilized according to each aim of examination.