The following report is submitted on the paper electrophoretic data obtained by 117 cases of gastric juice in several diseases.
(Method) The fresh gastric juice removed from an empty stomach in the morning was filtered by an aspirator, concentrated to 20:1 using the ultrafiltration method for 6 hours in the cold distilled water at negative pressure 240-300mmHg, and then electrophoreted. The protein was stained with Brom Phenol Blue (BPB), and mucoprotein was stained by Shiff's Periodic Acid Fuchsin Stain (PAS-reaction). Acid mucopolysaccharide was stained with Toluidine Blue solution at ph 2.5 and 7.0, and the characteristic “Metachromasia” was observed. Lipid was stained with Sudan IV.
(Results) In the gastric juice from healthy subject I found 5-6 low bands. In cases of acute and chronic gastritis, the electrophoretic patterns were not much different from the gastric juice of healthy subject.
Gastric cancer showed a significant characteristic pattern, different from gastric ulcer and others.
On the electrophoretic pattern of mucoprotein components in the gastric juice, 5 to 7 separate fractions were observed. Moreover, by the Toluidine Blue stain, it was found that the mucoprotein fraction with the highest mobility was containing acid mucopolysaccharides. This fraction may correspond with the “Glandular Mucopotein” of Glass and Boyd.
Only small amounts of lipids were found in the gastric juice with gastric cancer in 12 of the 16 cases, but in other diseases they could hardly be found.
These observations may be useful to the clinical diagnosis.
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