Normal rabbits were set up for artificial bile fistula just after the bilateral nephrectomy, and then the choleletic effects of Sodium dehydrocholate (abbreviated as “DHC”) were observed. The dose of DHC injected per kg of body weight was 1.0ml of 20 per cent aqueous solution. Whereas the effect of DHC injection was very notable at first, but at reinjection after three hours such a choleletic effect was no more seen. In order to prevent the decline of its effect at reinjection, cattle cystic bile, twice diluted with physiological saline solution, was, introduced into the intestine, but no improvement was obtain. Also the dried cattle bile on sale, which was diluted with 20 per cent glucose solution to 1 per cent solution, turned harmful. Alcoholic extract of the dried cattle bile remained still harmful and two out of three rabbits were sacrificed. On the contrary, the ether extract obtained from the residue after the alcoholic abstraction restored the choleletic effect of the DHC reinjection perfectly. Thus introducing the ether soluble ingredient of cattle bile into the intestine of rabbits, the outflow of electrolytes (Cl, Na) became also notable.
Liver function in the patients with slight and moderate pulmo nary tuberculosis were studied. I. Excretory Function. 1. Urobilinogen excretion in urin.; Positive instances above the medium degree were 22 of 73 cases (30.1%). 2. Bilirubin in serum.; Latent and abnormal impairments were 18 of 73 cases (24.7%). 3. Dye excretion test.; 19 of 73 cases (26.7%) showed retention of B.S.P. in serum. II. Protein Metabolism. 1. E.S.R.; 25 instances of 73 cases (34.2%) revealed increased E.S.R. 2. Takata Reaction.; 14 of 73 cases showed positive (19.2%). 3. Cadmium Reaction.; Reaction of shift to the left were 5 of 73 cases (6.8%), on the other hand, shift to the right were 11 of 73 cases (15.1%). III. The incidence of the hepatic dysfunction in the patients of pulmonary tuberculosis were as follows. 1. No significant differnce between both sexes. 2. Frequently found in the old, in those suffered long time, in severe cases, in moderate and emaciated patients, in those treated with artifical pneumo-peritoneum. Disabilities of Bilirubin and B.S.P. excretion were more often seen than disturbance of metabolic function. These excretory function tests were useful for discovery of latent impairment of liver function in the patients of pulmonary tuberculosis.
In an attempt to ascertain the behavior of the blood sugar throughout an entire day and to determine the action of P.Z.I., N.P.H. and insulin mixtures, blood sugar determinations were carried out at frequent intervals. When compared under similar conditions of diet and activity, same insulin in same dosage showed similar 24 hours blood sugar curve, and it was found that a single dose of N.P.H. brought similar curve as did a combination of unmodified insulin and P.Z.I. given by not separate injection but mixed in the syringe. P.Z.I. its effect began in the late afternoon, but there was no case of hypoglycemic tendency during the night. N.P.H. its effect indicated a more rapid onset and exerted into the evening and night so that the 24 hours curve was generally lower than P.Z.I.'s. In the course of the study the effect of giving various types of insulin indicated better in following order of P.Z.I., N.P.H., combination of unmodified 1 and P.Z.I. 2, combination of unmodified 1/3 or 1/2 and N.P.H. 1. Saving of depotinsulin could be expected in the case of under 10 units reqirement and could not in the more over insulin requirement. Administration of P.Z.I. in the morning before breakfast or bedtime, both showed similar 24 hours blood sugar curve. Diabetes in youth, its blood sugar values altered very much more than the adult so that use of insulin must be considered practically.
For the purpose of elucidating the relation between serum Ca and vitamin B1 in the body, the Ca content in serum of rabbits was reduced by inducing persistent hyper-or hypoglycemia or administering oxalic acid or acetic acid. Their subsequent total serum Ca, serum dialytic Ca, the drop number, the blood VB1 content and the excretion of VB1 in urine were measured. The serum Ca content and the VB1 content in the body in some diseases and pregnancy were also determined. Furthermore Ca was administered to subjects suffering from Ca deficiency in serum or VB1 deficiency and the subsequent VB1 metabo lism was followed up. The results obtained were as follows: 1) Adiminution of serum Ca disturbs the metabolism of VB1 in the body. Such a reduction of serum Ca content heightens the consumption of bodily VB1 on the one hand, and on the other lowers the utilization of the vitamin in the body. 2) This disturbance of VB1 metabolism caused by a diminition of serum Ca may be prevented or cured by administration of Ca An oral administration is more effective than otherwise. 3) Administration of VB1 is effective in preventing the reduction of serum Ca content and causes its slight rise. Therefore, it may be concluded that the Ca content in serum and the VB1 content in the body stand in mutual correlation.