Based upon Prof. Donomae's concept that the myocardial damage may occur at the early stage of focal infection in its clinical phase, the experiment was undergone to clarify the etiology of myocardial damage produced in focal-infected rabbits, from which the following results were obtained. 1) The repeated use of extract of focal lesion and heat-killed Streptococci has successfully produced the experimental carditis presenting the allergic responses simillar to those found in the focal infected rabbits by Aleuronat abscess. Accordingly, adding to the inoculated cocci, one of the significant etiology would be inflammatory products in focal lesion itself. 2) Protein fraction in focal lesion is more significant than polysaccharide and phosphatide for the onset of the disease. 3) Euglobulin is most significant among the protein fractions in focal lesion, that is, euglobulin, pseudoglobulin and albumin. 4) Protein fraction is most signific nt in each fractions being included in hemolytic streptococcus. 5) Streptolysin O may promote the disease, while streptokinase not.
In the previous paper, I reported that the K.I.K. factor (i. e. anemiogenic factor contained in cancerous gastric juice), unlike other cancer toxins, had no catalase depressing effect. In this report, I investigated the effect of K.I.K. factor on the rat liver phosphatase. The estimation of phosphatase activity was performed after Kay's method. The alkaline phosphatase activity was markedly depressed by injection of methanol precipitate as well as trichloracetic acid precipitate of cancerous gastric juice, but suffered no change by injection of trichloracetic acid supernatant. As the latter fraction corresponds to our K.I.K. factor, it is evident that K.I.K. factor lacks phosphatase depressing effect too.
In order to modify Dr. Nakagawa's test into a quantitative one, the author examined the elimination of the bile acid in the urine after its administration. In parallel with this test, some other liver function tests were performed. The results were as follows: 1) The liver function is expressed as the elimination quotient (E.Q.) of the bile acid, namely by D.E. (E.Q. of Dehydrocholic acid), R. E. (E. Q. of Reductodehydrocholic acid) and R.E./D.E., and when D.E.>3.0, R.E.<4.0 and R.E./D.E.<1.0, it is disturbed. 2) In cirrosis of the liver every patient showed a positive result in this test. 3) In acute hepatitis, the disturbances of the liver function by this test were rather remarkable. 4) In patients with enlargement of liver due to other diseases, this test showed positive results, too, reflecting the liver function accurately and sharply. 5) The results of this test paralleled, generally, those of some other liver function tests. 6) This tests is suitable to detect the latent disturbances of liver function.
It was presumed from many articles that the effects of hypophyseal adrenocortical hormones on blood sugar might change according to the experimental animals or in the case of human beings, by the existance of disturbances in carbohydrate metabolism. The authors have made an attempt to observe the changes in blood sugar for six hours after administration of these hormones to 55 diabetic patients in the fasting. The results obatined were as follows; (1) The effect of these hormones on blood sugar in diabetic patients could be classified in to three types: a) Falling type b) Rising type and c) Fluctuating type. (2) In most of these diabetic patients cortisone had a falling effect upon blood sugar and in no case was the effect one of rising. (3) The observation of the relation between types of diabetes and that of blood sugar curves after the injection of these hormones led us to the conclusion that no characteristic type of blood sugar curves among diabetic disease types were seen after injection of DCA with ascorbic acid, that after injection ACTH, there was an anti-insulin effect in extra-pancreatic diabetes, and a remarkable insulin effect in polyglandular diabetes respectively. On the other hand, cortisone, after injection, showed a remarkable insulin effect upon extrapancreatic diabetes. The authors believe that these characteristic types of blood sugar effects of ACTH and cortisone on diabetic patients will be available for distinguishing the types of diabetes.
One myelocytic leukemia was found as the result of hematological examinations which were performed on about 7000 persons who were exposed to the A-bomb within 2000m from the hypocenter. The patient was exposed to the A-bomb at a distance of 1200m when he was 2 years old. He had no major radiation symptoms and had been feeling well for the past 10 years. The patient was admitted to the hospital on 16-oct.-54. For observation, Anemia was not noticed, but the wbc increased to 47000 on 26-Oct. and the blood picture showed 13.5% promyelocytes, 1.0% myelocytes and 10.0% metamyelocytes in the neutrophilic series but no myeloblasts were seen. It was also noticed that the basophilic count was 5.5%. The bone-marrow showed 1.2% myeloblasts and 41.4%, promyeloffasts 8.0% myelocytes, 21.6% metamyelocytes, 12.6% stabs and 6.8% segs in the neutrophilic series. The incidence of juvenile cells was high; the lymphocyte count was 0.8%. The blood picture was that of myelocytic leukemia although the incidence of the myeloblasts was low. The spleen was palpable 1/2 finger breadths belon left costal margin and was of moderate consistency. The wbc showed a tendency to increase after admission and Nitromin was injected in a gradually increasing dosage to a total amount of 240mg. and was finally stopped because of the sudden decrease in the wbc and the juvenile cells. The patient still had no complaints and the wbc and the promyelocytes were arrested at the level of 10000 and 3-4% respectively. This case is of interest because of its being found in early stege in an A-bomb exposed person. The problem as to whether this is an acute or a chronic leukemia, and the increase of basophils must be followed in the future.
Fifty normal rabbits were determined for the serum and the bile. The concentration of electrolytes was slightly lower in the rabbit serum and bile than in the human serum and bile, and, in rabbits, the serum was a little more abundant in electrolytes than was the bile. The outflow of bile was observed on the rabbits which were nephrectomized and subjected 24 hours or shortly thereafter to artificial bile fistula, by introducing glass canules into their common ductus. The outflow and the electrolyt excretion in bile were larger in amount 24 hours after nephrectomy than immediately subsequent to it, being in the former almost two-fold as much as in the latter. The tendency was enhanced by the intravenous injection of Sodium dehydrocholate. Sodium dehydrocholate increased the excretion of chloride in the bile two-fold as much as befor it was given, although it lowered the chloride concentration in bile rapidly. As for the sodium, it entailed the increase in excretion without decrease in concentration. Repeated injection of sodium dehydrocholate was thought to be necessary for the complete compensation of liver for the lost function of kidneys, but its reinjection was unfortunately almost ineffective. However, the reinjection restored its effectiveness after rabbit bile had been introduced into the intestine. But reinjection remained ineffective when glucose and physiological saline were used instead of bile. Chloride concentration in bile commenced to rise one hour after the introduction of saline into the intestinal canal and ascended increasingly with the lapse of time for six hours. This seems to provide support for the hypothesis of enterohepatic circulation postulated by Rzentokowski. Similar effect was obtained when rabbit bile was used, presumably because of the chloride which was contained in it.
The purpose of the present paper is to report comparative observation of the variation on the level of potassium and inorganic phosphate in the plasma and urine of healthy and alloxan-diabetic animals by the administration of hexoses. Glucose, fructose and galactose were administered to normal and alloxan-diabetic dogs at a ratio of 2g and to rabbits at a ratio of 1, 5g per kilo. in a 20% solution intravenously. Duplicate analyses were made for the total sugar and the substances given, and for potassium and inorganic phosphate in the plasma and urine. The results of analyses showed as follows: 1) In the healthy dog and rabbit group, the ratio of utilization of those substances was ordinarily greatest in the case of glucose, less in fructose and least of all in galactose, 2) In the alloxan-diabetic animal group, the ratio of utilization of those substances was greatest in fructose, less in glucose and least of all in galactose ordinarily. 3) In the healthy animal group, the administration of hexoses produced a decrease in the concentration of potassium and inorganic phosphate in the plasma, and in the excretion rate of potassium and inorganic phosphate in the urine, although those decreases, expressed as a percentage of the orginal, were greatest in glucose, less in fructose and least of all in galactose. 4) In an alloxan-diabetic dog group, the administrtaion of hexoses produced a decrease in the concentration of inorganic phosphate in the plasma, although this decrease was smaller than that produced similarly in a normal dog group, but such administration did not change the concentration of potassium in plasma except in fructose. In an alloxan-diabetic rabbit group, the excretion rate of potassium and inorganic phosphate in urine showed no decrease except in fructose. 5) It seems to the worker that the decrease of potassium and inorganic phosphate in the plasma and urine vary in proportion to the ratio of utilization of those hexoses.
The permeability or absorption in the normal live rabbit has been investigated through the determination of the blood levels of various chemical substances infused into the bladder. The bladder-epithelium of rabbits leave out or absorbe foreign bodies, such as medical drugs, antibiotics and coloring matters, infused into the bladder, out of which are in hypertonic solution, and allows them to transfer into the body-fluid, especially into the blood stream, by its function of absorption or permeability. And the substances that have demonstrated their transference into the blood stream through the normal bladder-epitheliallayers of rabbits are as follows: Invertose, Glucose, 1-ascorbic acid, NaCl, CaCl2, sulfathiazole, sulfadiazine, arsenobenzene-sodium, procaine, hexamethylene-tetramine, P32, penicillin, streptomycin Dihydrostreptomycin, methylene-blue, Uranine, Hepatosulphalein etc. The velocity of transference of the substances infused into the bladder into the body-fluid is different and there are some variety of absorption.
We analysed hemodynamics of 13 hypertensive cases since August, 1954. We assumed 30 years old as the upper range of juvenile hypertension according to Master's statistics (1952) which is paid very discretion in dealing with the matrix. On an average, the systolic pressure of 13 patients was 152 mmHg., the diastolic 75 mmHg. Blood pressure level was as a rule labile and sometimes went down to normotension at the rest. In conformity to Wezler-Böger's classification contrasted with Fick-Cournand's method, 4 cases can be grouped into W form, and 9 into M form: E' form is not found up to date. In M form there were observed 6 cases with moderately high basal metabolism. In the group with elevated basal metabolism, the diastolic pressure was relatively low, the pulse pressure large, and the total peripheral resistance was decreased. Electolytes in serum were within normal range. In W form group there was one patient who showed hyperreactivity to both cold-pressor and histamine test. According to Keith-Wagener's classification, there was found one III type, and the others were grouped into I type. In Thorn's test eosinopenia registered 30% in 3 cases, and 9% in one patient. The patient who was of W form and III type was complicated by renal insufficiency. We have elucidated some differences hemodynamically between juvenile and senile hypertension, i.e. in former group while E'form was less dominant, there prevailed M form in which basal metabolism was often augumented and Methylthiouracil proved effective. En Passant in juvenile hypertension, there were found a few patients who showed malignant signs immediately from prehypertensive state.