A large number of follicles develop readily in the ovary of goats when stimulation is given by PMS injection. The intensity of this reaction depends on the dosage of injection. A dose higher than 1, 000 MU per animal is needed to expect the development of more than 10 follicles in total in both ovaries, and over 1, 500 MU for more than 30 follicles. Follicles induced by PMS injection do not reach ovulation, unless otherwise treated. This result is quite different from what has been experienced with ewes treated with PMS.

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1. The final reaction product obtained by the enzymatic degradation of hemoglobin-haptoglobin due to heme α-methenyl oxygenase has been characterized.
2. The product was failed to crystallize at the present time, however, the structure of heme part seems to be analogous to 656 mμ-absorbing substance, the product obtained by the degradation of pyridine-hemichromogen by the same enzyme.

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Color tests for the detection of hexosamines, N-acetylhexosamines, N-acetylneuraminic acid and other amino sugars on papers buffered with borate solutions are described. A method is described which permits the separation and identification of these sugars by paper electrophoresis.

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1. A crude enzyme preparation from Bac. Fulminans or from Bac. Cereus O(H) contained O(H)- and Le^b-decomposing enzymes, and when applied on an O(H)+, Le(a±b+) substance, destroyed O(H) and Le^b activity together with enhancing Le^a activity. But when applied on an O(H)+, Le(a±b-) substance, it only destroyed O(H) activity without enhancing Le^a activity. When, however, the O(H)+, Le(a±b+) substance was incubated with purified preparation from Bac. Fulminans, which contained only the O(H)-decomposing enzyme, abolishment of O(H) activity alone was observed, but not enhancement of Le^a activity.
These results seem to indicate the following with regard the process of formation of blood group substances: From a precursor substance are individually developed O(H) and Le^a substance, and on the basis of O(H) substance are formed A and B substance, and on the basis of Le^a substance is formed Le^b substance.
However, when the crude enzyme preparation from Bac. Fulminans or from Bac. Cereus O(H) was applied on OLe(a-b+) red cells, it was difficult to recognize appearance of Le^a activity, though O(H) and Le^b activity were abolished.
2. When the A-decomposing enzyme from Cl. Tertium and the B-decomposing enzyme from Cl. Maebashi were applied on A and B substance or red cells with Le^b activity, respectively, slight increase in Le^b activity was observed together with enhancement of O(H) activity. When AB substance or AB red cells were incubated with both the A- and B-decomposing enzymes, these were transformed into a substance or red cells having O(H) activity nearly to the same degree as that of O(H) substance or O red cells.

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1. A purified O(H)-decomposing enzyme from Bac. fulminans, when incubated with O red cells or crude glycolipid extracted from them, released fucose, as the result abolishing their reactivity with anti-O(H) antibody in eel serum, and instead enhancing antigenicity common to Sh. Dysenteriae. The O(H)- and Le^b-decomposing enzyme from Bac. Cereus released from the same materials fucose and galactose, consequently abolishing the O(H) activity completely, and instead enhancing antigenicity common to Dip. pneumoniae Type XIV.
2. From the amount of fucose liberated from red cells as the result of the enzyme action, the molecule number of sugar as the terminal determinant of the O(H) specificity per one red cell was computed.

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The titration score of ABO antibodies in mercapto-treated sera corresponds well with the severity of hemolytic disease of ABO incompatible infants. Adoptation of this “mercapto-test” as an ABO incompatibility test will make possible prediction of infant's hemolytic disease and facilitate preparation for treatment.

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