In ovariectomized adult C57 Black/MS mice which had been given daily injections of 20μg estradiol for 5 days from the day of birth, the vaginal epithelium underwent persistent hyperplasia and cornification. However, the vaginal epithelium of ovariectomized adult mice receiving 5 daily injections of 20μg estradiol concurrently with 100 I.U. vitamin A acetate was atrophic, like that of ovariectomized mice given daily injections of either vitamin A alone or the vehicle (sesame oil) during the same neonatal period. In ovariectomized adult mice which had been given 5 daily injections of 20μg estradiol from the day of birth and 5 daily injections of 100 I.U. vitamin A from the 6th day on, the vaginal epithelium was stratified and cornified as in mice receiving neonatal injections of estradiol only.
A chromosome translocation involving two D chromosomes was found in 2 patients, one a phenotypically normal female (case 1) who experienced 3 consecutive abortions and the other a male (case 2) with hypogonadism. Autoradiographic analyses strongly indicated that the abnormal metacentric chromosome was resulted from the centric fusion between D13 and D14 chromosomes in both cases. It appears that the translocation per se does not deal with phenotypical anomalies.