Rinsho Ketsueki
Online ISSN : 1882-0824
Print ISSN : 0485-1439
ISSN-L : 0485-1439
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Volume 24, Issue 7
Displaying 1-30 of 30 articles from this issue
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  • Production of Myeloid Cell-Specific Antiserum from HL-60 Cell Line and its Clinical Application
    Hajime NAKAZAWA, Rokuichi IGA, Keiko TAKANE, Taijiro MORI, Shinpei NAK ...
    1983 Volume 24 Issue 7 Pages 812-817
    Published: 1983
    Released on J-STAGE: January 26, 2009
    DOIhttps://doi.org/10.11406/rinketsu.24.812
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    The myeloid leukemia cell line, HL-60, was immunized to the New Zealand White rabbit and the anti-HL-60 serum was made as follows: 5×108 of HL-60 cells were incubated with the anti-B cell serum for 1 hour at 4°C, washed with PBS (-) twice, resuspended in 5 ml of PBS (-), and injected intravenously to the rabbit. After 6 weekly injections, the rabbit was bled 1 week after the last injection.
    After extensive absorption with red blood cells, T-, B-, and null cell lines, the anti-HL-60 serum was proved to be specific for myeloid cells including HL-60, K-562, myeloblasts, and normal granulocytes.
    The leukemic cells from the patients with several types of leukemias were tested by the method of the membrane immunofluorescence using the anti-HL-60 serum as the reagent. The antiserum reacted to the myelogenous leukemic cells and not to the lymphoid leukemic cells. It was indicated that the anti-HL-60 serum was usefull in determining the leukemic type especially when it was not dicided by the other examinations.
    The fact that the antiserum reacted to not only the myeloid leukemic cells but also the normal granulocytes may suggest the presence of the so-called “myeloid antigen.”
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  • Some Clinical Aspects of Adult T-cell Leukemia-Lymphoma (ATLL)
    (1) Duration of Survival of Adult T-cell Leukemia (ATL) and Some Useful Parameters
    Kenichiro KINOSHITA, Shuichi IKEDA, Yasuaki YAMADA, Tatsuhiko AMAGASAK ...
    1983 Volume 24 Issue 7 Pages 818-825
    Published: 1983
    Released on J-STAGE: January 26, 2009
    DOIhttps://doi.org/10.11406/rinketsu.24.818
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    The duration of survival of adult T-cell leukemia (ATL) was correlated with the level of serum LDH, histologic subtype, leukocyte count and labelling indices (LI) of the leukemic cells as determined by 3H-TdR autoradiography at the time of diagnosis.
    The results were as follows.
    1) Statistical analysis revealed that the patients with the histology of medium cell-sized type had a longer survival rate than the patients with that of plemorphic or large cell type.
    2) A highly significant difference was found between the survival pattern of the patients with LDH levels less than 500 U and those with LDH levels greater than 700 U. (Mean survival rates were 7 months and 17 months, respectively.) On the other hand, the duration of survival of the patients with LDH levels from 500 to 700 U was variable.
    3) A significant correlation was found between LI of the leukemic cells from the peripheral blood or lymph nodes and the levels of LDH.
    4) The duration of survival adversely correlated with elevation of leukocyte count and most of the patients with leukocyte count more than 3.5×104/μl died within one year.
    Pretreatment serum LDH determination may provide a useful parameter of disease activity and prognosis of ATL patients, and it was suggested that we should determine the choice of chemotherapy for ATL on the basis of LDH levels, histologic subtype and leukocyte count.
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  • Studies on Hematopoietic Stem Cells in Patients with Rheumatoid Arthritis
    Tsutom SHICHISHIMA, Toshiyuki ISHIBASHI, Hiroyuki KANBAYASHI, Keiji KO ...
    1983 Volume 24 Issue 7 Pages 826-833
    Published: 1983
    Released on J-STAGE: January 26, 2009
    DOIhttps://doi.org/10.11406/rinketsu.24.826
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    In order to know the cause of anemia and leucopenia in patients with rheumatoid arthritis, in vitro cultures of hematopoietic stem cells were evaluated.
    Hundred thousand and 2×105 bone marrow cells were used for CFU-E and for CFU-C assay. The numbers of colonies in both culture systems were significantly decreased in rheumatoid arthritis comparing with normal control.
    Serum or peripheral T lymphocytes of patients with rheumatoid arthritis were added to CFU-E or CFU-C culture system of normal bone marrow cells. The addition of patients's serum to CFU-E culture system caused remarkable deppression in number of colonies. In several cases, suppression of colony formation was also observed in other combination. In T lymphocytes depleted bone marrow cells of rheumatoid arthritis, the number of colonies increased in both CFU-E and CFU-C culture system in some cases.
    From these findings, it is suggested that the proliferation and differentiation of erythroid and granulo-macrophage precursor cells might be disturbed by patient's serum or peripheral and bone marrow T lymphocytes. These mechanism may be one of the causes of anemia and leucopenia in rheumatoid arthritis.
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  • The Effects of Corticosteroids and Erythrocyte Sedimenting Agents on Centrifugal Leukapheresis
    Hisahiro SAKAMOTO, Mikiko ABE, Masaru SHIMIZU
    1983 Volume 24 Issue 7 Pages 834-841
    Published: 1983
    Released on J-STAGE: January 26, 2009
    DOIhttps://doi.org/10.11406/rinketsu.24.834
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    One hundred and sixty three healthy donors were leukapheresed by a CFC or IFC blood cell separator, and the following data were obtained.
    For CFC, 1.78-1.74×1010 granulocytes were collected with premedication of dexamethasone (DX) 7mg or 5mg followed by hydrocortisone (HC) 100mg and using HES with molecular weight 20,000 (LMW).
    For IFC, the yields of granulocytes were 1.49-1.43×1010, by collecting the red cell layer for 4 minutes at 20ml/min. of flow rate per cycle, with premedication of DX 5mg alone or DX 5mg followed by HC 100mg and LMW-HES or modified fluid gelatine (MFG).
    Urinary sugar tests were + or 2 + in thirty (24.4%) out of one hundred and twenty three donors premedicated with DX.
    In eight (30.8%) out of twenty six donors administered MFG, protein was detected in their urine.
    On biochemical examination, the level of inorganic phosphorus (IOP) in the sera decreased about 50% in the donors administered either LMW-HES or MFG. About 30% decrease of the serum IOP level was found in the sera of the donors administered only ACD-A solution in thrombocytapheresis. This decrease of the IOP level dit not occur in the serum of a healthy donor who was experimentally infused 500ml of LMW-HES only, although infusion of 500ml saline or LMW-HES containing 30ml of 46.7% trisodium citrate did cause the decrease of his serum IOP.
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  • Differentiation of Human Acute Leukemia Cells and Its Usefulness for Clinical Diagnosis
    Masatsugu OHTA, Masaki SAITO, Seiichi KITAGAWA, Yasusada MIURA, Fumima ...
    1983 Volume 24 Issue 7 Pages 842-849
    Published: 1983
    Released on J-STAGE: January 26, 2009
    DOIhttps://doi.org/10.11406/rinketsu.24.842
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    Recently-established human myeloid leukemia cell lines such as K 562, HL-60 and KG-1 can be induced by various chemical inducers to differentiate into myeloid mature cells or macrophage-like cells. Among them, 12-0-tetradecanoyl phorbol-13-acetate (TPA), a potent tumor promoter, was most potent for the induction of terminal differentiation of human myelogenous leukemia cell lines into the latter type of mature cells. In this communication, we studied morphological and functional changes of human leukemia cells freshly obtained from 11 acute non-lymphocytic leukemia and 5 lymphocytic leukemia patients, using TPA, retinoic acid or dimethyl sulfoxide.
    The myeloid leukemia cells cultured with TPA became adherent to plastic petri dish, and then developed macrophage-like morphology with long filamentous pseudopods. They showed a marked enhancement of ability to phagocytose latex particles. These three properties acquired, i.e. the cell adherency, the extension of pseudopods and the enhancement of nonimmunogenic phagocytic activity, did not always parallel each other. In some cases of myeloid leukemias, retinoic acid significantly increased phagocytic activity without iduction of adherency.
    On the other hand, when cultured with the above inducers, the lymphocytic leukemia cells did not show morphological and functional changes except that, in some cases, cell aggregates were observed.
    These characteristic changes were also observed in leukemic cells from blastic crisis of chronic myelogenous leukemia: the blast cells of myeloid crisis became adherent to plastic culture dish with long filamentous pseudopods, showing an increase in phagocytic activity when induced by TPA, and the blast cells of lymphoid crisis did not show such morphological and functional changes.
    It is suggested that an exposure of leukemic blast cells to TPA for relatively short times (12 to 24 h) may be useful for determining whether they are of myeloid or lymphoid origin, and for the subsequent choice of adequate chemotherapies for the acute leukemias.
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  • Locomotion, Phagocytosis and Bactericidal Activity of Granulocytes during Lithium Therapy
    Tadashi OJIMA, Tadatoshi KURATSUJI, Shunichi SHIMIZU, Ja Yung LEE, Eis ...
    1983 Volume 24 Issue 7 Pages 850-854
    Published: 1983
    Released on J-STAGE: January 26, 2009
    DOIhttps://doi.org/10.11406/rinketsu.24.850
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    Therapy with lithium carbonate induces granulocytosis in psychiatric patients, and it has recently been used to reverse the primary neutropenia and secondary neutropenia associated with cancer therapy.
    The purpose of this study is to clarify the effects of lithium therapy on neutrophil functions. Chemotaxis, random mobility, deformability, NBT reduction and chemiluminescence were measured in neutrophils taken from 4 patients being successfully treated with lithium therapy, as well as in control's cells taken from healthy donors. No significant differences were found. Addition of lithium carbonate to normal neutrophils in vitro had no significant effect on cell functions.
    These findings suggest that therapy with lithium carbonate is useful in the treatment of neutropenic states.
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  • Remission Induction Failures in Adult Acute Leukemia
    Kohei KAWASHIMA, Ei-ichi NAGURA, Hisamitsu SUZUKI, Eiji WATANABE, Yuki ...
    1983 Volume 24 Issue 7 Pages 855-861
    Published: 1983
    Released on J-STAGE: January 26, 2009
    DOIhttps://doi.org/10.11406/rinketsu.24.855
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    A study was undertaken to derive useful informations from therapeutic failures in cases with acute leukemia by analysing a distinction between the various modes of clinical failures. Between January 1970 and December 1981, 60 adult patients with acute leukemia were judged to be therapeutic failure. According to schema for the classification of remission induction failures proposed by Preisler, they were subdivided into 6 types; Type I: absolute drug resistance, Type II: relative drug resistance, Type III: regeneration failure, Type IV: death during hypoplasia, Type V: inadequate trial of therapy and Type VI: extramedullary persistance. Case numbers of each type were 16, 18, 3, 5, 15, and 3, respectively. The ratio of female to male was 1: 1.3. Between 1970 and 1972, Type I was predominant in the distribution of failure types, but in the later periods, the distribution shifted to Type II and V. Failure types of both AML and AMoL were occupied mainly by Type I and II whereas those of AProL was occupied by Type V. There were no correlations between hematological findings at diagnosis and failure types. However, the presenting features at diagnosis affected the distribution of failure types, i.e. Type I in high fever episode and Type V in DIC episode. In terms of remission induction therapy, 27% of DCMP regimen was Type I failure, but with more intensive regimen, Type II failure increased remarkably instead of Type I which occupied 13.2%. The main cause of death in remission induction failures was as follows; sepsis and pneumonia in Type I an II, intracranial hemorrhage in Type II and V. Using this classification schema, it was possible to analyse the mechanism of treatment failure and seemed to lead to logically derived therapy studies.
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  • Immunological Studies on G6PD Ube
    Yukari MIURA, Yoji ISHIDA, Koji NAKASHIMA, Noboru MATSUMOTO, Toshio KA ...
    1983 Volume 24 Issue 7 Pages 862-867
    Published: 1983
    Released on J-STAGE: January 26, 2009
    DOIhttps://doi.org/10.11406/rinketsu.24.862
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    G6PD variant (G6PD Ube), not associated with hemolytic anemia was analysed by the methods of antibody neutralization, electroimmunodiffusion and immunodiffusion.
    Antibody neutralization revealed that G6PD in G6PD Ube is slightly difficult to be neutralized by antibody of normal G6PD compared to normal G6PD.
    Electroimmunodiffusion and immunodiffusion methods showed that activity/immunoprecipitation is decreased (86% and 85% of normal control respectively) and that antigen/Hb is also decreased markedly (41% of normal control).
    These results on G6PD Ube suggest that this variant has absolutely decreased production of protein in addition to the decreased activity per one molecule due to abnormal molecular structure.
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  • A Case of Thrombotic Thrombocytopenic Purpura (TTP) Showing a Deposition of IgM in Small Arterial Walls of the Spleen
    Masayuki ARASHI, Yoshikazu AKAHONAI, Mitsuo IKEBE, Yuji HINODA, Tetsuo ...
    1983 Volume 24 Issue 7 Pages 868-873
    Published: 1983
    Released on J-STAGE: January 26, 2009
    DOIhttps://doi.org/10.11406/rinketsu.24.868
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    A typical case of thrombotic thrombocytopenic purpura (TTP) was reported in which an immunological mechanism was suspected to be relevant to its etiology.
    A 31-year-old man had a sudden onset of general malaise, vertigo, headache and bleeding tendency. He was immediately admitted to our hospital because of convulsion and disturbance of consciourness. Laboratory examinations revealed obvious anemia and hemorrhagic diathesis in association with microangiopathic hemolytic anemia characterized by red cell fragmentation and nucleated red blood cells. Coagulation time, fibrinogen, factor VIII and antithrombin III were within normal limits while thrombocytopenia and a mild elevation of FDP were observed. He was treated with prednisolone, fresh frozen plasma and heparin on a diagnosis of TTP, but bleeding tendency and coma progressively increased, and he died 11 days after the onset of symptoms.
    Aspiration autopsy specimens of the spleen revealed multiple thrombi at the small arteries and the proliferation of endothelial cells of these arteries. In addition, a deposition of IgM and a small amount of C3 was observed in the endothelium of small splenic arteries.
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  • Familiar Occurrence of Hypoplastic Leukemia in Brothers
    Maki KUROKAWA, Masamichi AMANO, Yukio IMAMURA, Hiroshi OOAMI, Seitoku ...
    1983 Volume 24 Issue 7 Pages 874-880
    Published: 1983
    Released on J-STAGE: January 26, 2009
    DOIhttps://doi.org/10.11406/rinketsu.24.874
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    Hypolastic leukemia in brothers is reported.
    First case, a 54-year-old man admitted to our hospital in July, 1974 because of general fatigue and palpitation. There was no hepatosplenomegaly and no bleeding tendency. Blood counts revealed white cell count of 1,300 with 23% neutrophils and 75% lymphoid cells. The hemoglobin was 5.6 g/dl. The bone marrow was quite hypocellular with about 20% myeloblasts with very fine chromatin, prominent nucleoli and no Auer body. A diagnosis of hypoplastic myeloid leukemia was made. The patient was treated with blood transfusions and protein anabolic steroid. In the fourty admission for bloodtansfusion, fever and hematomesis suddenly occurred. He died of pneumonia and bleeding of stomach in May, 1975.
    Second case, a 56-year-old man, his younger brother, was admitted in February, 1980 because of anemia. The liver edge was felt 1.5 cm below the costal margin, but the spleen was not palpable. There was no lymphoadenopathy and no bleeding tendency. Blood counts revealed white cell count of 1,500 with 32% neutrophils and 62% lymphoid cells. The hemoglobin was 9.2 g/dl. The bone marrow was quite hypocellular with about 20% myeloblasts with very fine chromatin, prominent nucleoli and no Auer body. This finding was very similar to those of the first case. A diagnosis of hypoplastic myeloid leukemia was made. He was treated with blood transfusions. In the seventh admission for blood transfusion in July, 1982, the bone marrow was normocellular with 75% myeloblasts. But his peripheral blood was not changed. Inspite of chemotherapy-vindesin, cyclophosphamide, plednisolone-, he was dead of sepsis of Candida in November.
    These two cases were very similar in aspects of age, sex, chief complaints, bone marrow findings, peripheral blood counts, and clinical course.
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  • A Case of Acute Myeloblastic Leukemia with Transient Loss of A and H Antigens of the Red Blood Cells
    Takayoshi ASAI, Kuniaki ITO, Yuri SUGIURA, Hakumei O, Seitoku FUJIOKA
    1983 Volume 24 Issue 7 Pages 881-886
    Published: 1983
    Released on J-STAGE: January 26, 2009
    DOIhttps://doi.org/10.11406/rinketsu.24.881
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    A 43 yr-old female was admitted to our hospital with the complaint of gingival swelling and bleeding in April, 1967. Laboratory examination revealed RBC 232×104/cmm, Hb 7.9 g/dl, WBC 49,500/cmm (Myeloblast 80%, peroxidase (+), Auer body (+)), platelet 4,640/cmm, reticulocyte 13%. In bone marrow, myeloblast was 79%. She was diagnosed to be acute myeloblastic leukemia, and treated with ACMP and COAP regimens, entering into complete remission after 3 months.
    On the first admission, the examination on blood cell type prove to be O type. O type blood was transfused. On the second admission with relapse, her blood cell type was found to be A type, and her serum taken on the first admission and stored at -20 degrees was reexamined to find anti-A antibody.
    The examination of red cell type on smear cells in her course suggest us that A and H antigens dissapeared on the first admission and reappeared in complete remission.
    Her blood cell type was A at labor of her son. Her husband had O type red cells and her two sons A type. At her relapse she was transfused A type blood, but her blood cell type never changed.
    On September 12, 1968, she died of cryptococcus meningitis.
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  • A Case of Hypereosinophilic Syndrome
    —with Special Reference to CFU-eosino—
    Kohji TANEICHI, Takahiko KONNO, Hidetoshi SHIBAKI, Ken-ichi SAWADA
    1983 Volume 24 Issue 7 Pages 887-891
    Published: 1983
    Released on J-STAGE: January 26, 2009
    DOIhttps://doi.org/10.11406/rinketsu.24.887
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    A 41-year-old male was admitted to the hospital with complaints of cough, diarrhea and itching of the skin. The physical examinations on admission showed scarlet papules at hands, foot and forearms. Lymphadenopathy at left axillar and bilateral inguinal area and edema on bilateral hands and lower extremities were observed. The laboratory findings were follows: WBC 9,200/mm3 with 30% of eosinophils. The results of frequent examination for ova of feces were negative. The slight splenomegaly was observed on scintigram. After admission the count of eosinophils increased rapidly and reached to 12,600/mm3. Clinical manifestations, such as cough, exanthema, angioneurotic edema, diarrhea and melena persisted. Basic diseases causing hypereosinophils were not found out. On the basis of these findings, the diagnosis of hypereosinophilic syndrome was made. Administration of predonisolon by 30 mg a day was begun, resulting in excellent effects for clinical and laboratory manifestations. CFU-C and CFU-eosino of bone marrow cells were examined, resulting in normal ranges. This result supports the fact that the patients with hypereosinophilic syndrome having normal range of CFU-eosino are reactive with corticosteroid therapy.
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  • Hemolytic Anemia Caused by Infusion of Factor VIII Concentrates—A Case Report of Hemophilia A with Intracranial Bleeding
    Keiji FUKAZAWA, Toshiya OKUYAMA, Hideharu MANABE, Junko KANAYA
    1983 Volume 24 Issue 7 Pages 892-896
    Published: 1983
    Released on J-STAGE: January 26, 2009
    DOIhttps://doi.org/10.11406/rinketsu.24.892
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    A 20-year-old man with severe hemophilia A developed hemolytic anemia while he was receiving factor VIII concentrates from pooled plasma (Conco-eight) for his subdural hematoma. Although the direct Coombs' test was negative, the hemolysis was considered due to the iso-agglutinins (anti-A antibody) contained in the concentrates. After the concentrates was changed to the blood group-specific one (Hemophil), the hemolytic anemia improved. A total of 56,000 units of factor VIII was administered (Conco-eiht: 48,000 units, group-specific Hemophil: 8,000 units) and the subdural hematoma was absorbed with no sequelae.
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