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Volume 7 (1966)
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Volume 7, Issue 6
Displaying 1-11 of 11 articles from this issue
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[in Japanese]1966 Volume 7 Issue 6 Pages 507-512
Published: 1966
Released on J-STAGE: October 20, 2008
JOURNAL RESTRICTED ACCESSDownload PDF (576K)
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Matsuzo MATSUOKA, Nobuo SAKURAGAWA1966 Volume 7 Issue 6 Pages 513-521
Published: 1966
Released on J-STAGE: October 20, 2008
JOURNAL RESTRICTED ACCESSThe fibrin plate method (Astrup & Müllertz 1952, Lassen 1953) is generally used for measuring fibrinolytic activity of various materials.
This paper presents the results of experimental studies on fibrin substrates used in this method and some factors which concerning fibrinolytic activity of material.
(1) The human plasma (Nihon-Seiyaku), human fibrinogen (Midori-Juji) and bovine fibrinogen (prepared according to Astrup & Müllertz 1952) were used as substrates. In human plasma plasminogen-proactivator, plasminogen and anti-plasmin were contained, and in human fibrinogen plasminogen-proactivator and plasminogen were inclosed. But in bovine fibrinogen only slight plasminogen could be proved, which was its advantage for being the substrate of this method. This bovine fibrinogen could be used for a period of 3 weeks when kept frozen at -20°C.
(2) The fibrinolytic activity measured was influenced by fibrin concentration in the substrate, the amount of tested material and reaction temperature, the best conditions being 0.1% fibrin plate, 0.03ml. of tested materials and at room temperature (20°C) for 18 hours.
(3) Errors in measuring fibrinolytic activity, which could be induced by above described factors, were avoided if “fibrinolytic ratio” (=lysis area of material activated with urokinase/lysis area of urokinase alone×100%) was used. To have the absolute fibrinolytic activity of plasminogen-activator and plasminogen-proactivator in tested materials, the lysis area of the mixture of streptokinase and tested materials on unheated plates were measured when the lysis area of control material (urokinase alone) reached a certain arbitrary size (for instance 400 mm2).
(4) There were found some residual urokinase in the plasmin material which was produced by urokinase activation of humen plasminogen, but it could be applied for measuring anti-plasmin potency in materials such as human plasma and t-AMCHA solution of over 12.5 mg/ml. Antiplasmin potency could be measured more quantitatively by unheated plate than by heated plate.View full abstractDownload PDF (773K) -
Part I. Fibrinolytic Activity of Bone Marrow Aspiration of Patients with Various Blood DiseasesAtsumasa HANZAWA, Eiichi KUYAMA1966 Volume 7 Issue 6 Pages 522-526
Published: 1966
Released on J-STAGE: October 20, 2008
JOURNAL RESTRICTED ACCESSBy means of the standard and heated fibrin plate method the fibrinolytic activity of bone marrow from patients with various blood diseases was studied. For the measurement of fibrinolytic activity the euglobulinic precipitates obtained from the venous blood and the bone marrow aspiration were used.
In normal and pathological conditions the level of plasmin activity of bone marrow was higher than of peripheral blood and specially in cares with acute leukemia, macroglobulinemia, multiple myeloma and essential thrombocytopenic purpura the plasmin activity was remarkable.
The level of plasminogen activator in bone marrow was also higher than in venous blood. Leukopoietic and thrombocytopoietic disorders as well as dysproteinemias showed markedly high activator activity. And it was imagined from these findings that plasminogen might be activated to plasmin in bone marrow.View full abstractDownload PDF (408K) -
Third Report: On the Bone Marrow Findings in 20 Cases of Gastric Cancer and Review of Literatures on Pathogenesis of Anemia in Gastric CancerSaburo HARASHIMA1966 Volume 7 Issue 6 Pages 527-532
Published: 1966
Released on J-STAGE: October 20, 2008
JOURNAL RESTRICTED ACCESSThe nucleated cell count in bone marrow of 18 gastric cancer cases showed decrease in 12 cases and megakaryocyte count was decreased in 6 of 11 cases. Studies on the myelogram in 20 cases revealed increases of neutrophilic promyelocytes, plasma cells, and reticulum cells. Neutrophilic segmented cells and eosinophilic leucocytes were found decreased in number. No significant change was observed in basophilic leucocyte, monocyte, lymphocyte, and erythroblast countings. The differentiation of neutrophilic system revealed disturbance of maturation in 16, aplasia in 2, and normal figure in 2 cases. Study of the erythrocyte system showed 4 cases of aplasia and one case of hyperplasia.
The shortened red cell survival time, nutritional disturbance, disturbance in iron metabolism, bone marrow injury and bleeding were thought to be responsible for the development of the anemia in gastric cancer cases.View full abstractDownload PDF (619K) -
Ichiro NISHIZAWA1966 Volume 7 Issue 6 Pages 533-539
Published: 1966
Released on J-STAGE: October 20, 2008
JOURNAL RESTRICTED ACCESSOral administration of iron to 14 cases of RA was proved effective in nine and non-effective in five patients. The differences between the effective and non-effective groups were found in Hb value before iron administration and in SCu after four weeks of iron therapy. No other differences could be shown in this report. From this result, iron effective group was proved to have a milder anemia than non-effective one.
Further study was performed with an intention to show the differences between the anemia of RA and iron deficiency anemia. Twenty-seven RA patients, having moderate anemia, were compared with twenty-three shopgirls of a department store, who showed as low Hb value as the RA group and good response to oral administration of iron. The differences between the RA group and the iron deficiency group were found statistically significant in Ht, MCV, MCHC, TIBC, SCu and serum albumin. But there were no significant differences in RCC, MCH and SFe.
The morphological difference in red blood cells was notable, i.e. the anemia of RA was microcytic, while iron deficiency anemia was hypochromic. This morphological difference was thought due to lacking substances. The Hb of RA group showed a remarkable correlation to serum albumin, while MCHC in iron deficiency anemia correlated to SFe.
The analysis by the discriminant function showed that Ht played an important role to differentiate the anemia of RA from iron deficiency anemia.View full abstractDownload PDF (551K) -
Studies on absorption of iron, administered orally in the form of “shotgun” anti-anemic preparation.Akinobu WATANABE, Shinpei FURUSAWA, Kenichi KAWADA, Yoshiichi SUGA1966 Volume 7 Issue 6 Pages 540-542
Published: 1966
Released on J-STAGE: October 20, 2008
JOURNAL RESTRICTED ACCESSOne hundred mg of ferrous sulphate was administered orally mixed with 5 μc 59FeSO4 (0.084 μg iron) to 4 normal subjects and 2 patients with iron deficiency anemia in order to determine iron absorption using the double isotopic technique. The iron absorption rates were 6.0±3.8% in the normal subjects and 38.0 and 56.7% respectively in the anemic patients.
The other group of 4 normal subjects and 2 iron deficient anemic patients was given 2 mg of folic acid, 50 mg of vitamin C and 3 μg of vitamin B12 together with the iron preparation, and the iron absorption rates were 10.0±7.8% in normal and 54.5 and 42.8% in two anemic patients respectively.
The results revealed that there was no significant difference in the iron absorption rate between the two groups.View full abstractDownload PDF (216K) -
I. Clinical observation on bone marrow aplasia during myleran therapyKiku NAKAO, Keiji KINUGASA, Yukio IMAMURA, Masao HATTORI, Seitoku FUJI ...1966 Volume 7 Issue 6 Pages 543-548
Published: 1966
Released on J-STAGE: October 20, 2008
JOURNAL RESTRICTED ACCESSAmong 19 cases of chronic myelogenous leukemia who had been treated with standard dosis of myleran during 1957 and 1965, 3 cases developed a severe bone marrow aplasia, and one of these aplastic cases succumbed with intrapulmonary hemorrhage.
The bone marrow aplasia became apparent within 2 months after the termination of myleran therapy.
These aplastic cases did not show any hematological signs suggesting the occurrence of bone marrow aplasia before and during the myleran therapy except that the shrinkage of the spleen in response to the therapy was rather marked and that the thrombocytopenia became severe even after the cessetion of therapy.
Although the steroid therapy was effective in ameliorating the anemia, it did not show any apparent effect on the recovery from thrombocytopenia.View full abstractDownload PDF (491K) -
Akira ISHIKAWA, Tadayuki YAMBE, Yoshio KOSHINO, Sohko MACHIDA, Yoshima ...1966 Volume 7 Issue 6 Pages 549-554
Published: 1966
Released on J-STAGE: October 20, 2008
JOURNAL RESTRICTED ACCESSStudies were performed on the level of serum erythropoietin in cord blood and in newborn infants.
Nineteen blood specimens from cord and 22 from neonates aged from four to 125 hours were investigated as well as 68 specimens cords of healthy full-term newborns.
For the assay the radioactive iron uptake into circulating erythrocytes in the fasted rat was determined and the percentage of radioiron in the blood to the total administered dosis employed as the indicate of erythropoiesis.
Thirteen specimens out of 19 from cord blood showed mild moderate increase in erythropoietin level. Approximately, in 68.4% of serum specimens from cord blood erythropoietin was elevated, but no elevation was observed in the neonatal specimens, 10 of which were obtained within 10 hours after delivery.
The fact that the elevated erythropoietin in cord blood was depressed rapidly after birth, might suggest the role and significance of anoxic anoxia in the production of erythropoietin.View full abstractDownload PDF (506K)
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Susumu MITSUTANI, Hidetoshi MATSUMOTO, Susumu IMOTO, Kenji MIYAMA1966 Volume 7 Issue 6 Pages 555-561
Published: 1966
Released on J-STAGE: October 20, 2008
JOURNAL RESTRICTED ACCESSA 34-year-old housewife, complaining of cough for about 6 months and of fatigue, palpitation and pale face for about a month, was admitted to our hospital on Dec. 21st, 1965. On admission, severe hemolytic anemia and thrombocytopenia were proved. Antiglobulin tests of red cells and antiglobulin consumption tests of platelets showed negative. The bizarre poikilocytes such as burr cell, helmet cell, triangular cell and schistocyte were noticed on the peripheral blood smear. Bone marrow picture exhibited markedly increasing of both erythropoiesis and thrombopoiesis. G-6-P DH activity of red cells was normal. Corticosteroid, hemostatica and anti-plasmin agent etc. were not effective, she was expired on Jan. 20th, 1966.
The postmortem examination revealed wide spread metastatic adenocarcinoma provably originated from ovarial cancer (2 years ago, r-ovariectomy had been performed in the other hospital on the diagnosis of dermoid cyst). Moreover, tumor cell emboli in lung blood vessels and multiple thrombi in small blood vessels in the lung, heart and kidney were noted.
These clinical and pathological findings were similar to those of thrombotic thrombocytopenic purpura, and appeared to favor the concept of microangiopathic hemolytic anemia recently Brain et al. had postulated.View full abstractDownload PDF (1072K) -
Toshio SHINODA, Kin-ichi NAKAMURA, Mitsuto HASEGAWA1966 Volume 7 Issue 6 Pages 562-567
Published: 1966
Released on J-STAGE: October 20, 2008
JOURNAL RESTRICTED ACCESSA case of thymoma with leukemic character was reported. The patient, a 22 year old man was admitted in August, 1964. He had been anemic for three years and febrile for one month before admission.
His peripheral blood showed a remarkable pancytopenic figure dominantly occupied with peroxydase-negative lymphoid cells. Bone marrow studies revealed hypocellularity which was dominated by lymphoid cells. Coombs' test was negative. X-ray examination revealed destruction of the left clavicula. Treatment with blood-transfusion, prednisolone and antibiotics showed no effect. He died 5 days after admission.
Post-mortem examination revealed thymoma (60 gm) of lymphocyte dominant type. Infiltration of lymphoid cells was found in the bone marrow, liver and kidney, not in the spleen. All the lymphnodes examined were normal in structure, except only one, in which a structure like Hassal's body was found surrounded by infiltration of lymphoid cells. In the hypocellular bone marrow were found reticulum cells, plasma cells and diffusely infiltrated lymphoid cells, and almost none of granulocytes.
Infiltration pattern, morphological findings of lymphoid cells and histological findings of the tumor suggested that thymic lymphoid cells was infiltrated leukemically in nature.
The possibility of the existence of thymic leukemia and the relation of pancytopenia and thymoma were discussed.View full abstractDownload PDF (1098K) -
Atsuo SUZUKI, Akira SHIBATA, Seiju ONODERA, Akira B. MIURA, Shinobu SA ...1966 Volume 7 Issue 6 Pages 568-572
Published: 1966
Released on J-STAGE: October 20, 2008
JOURNAL RESTRICTED ACCESSA thirty-three-year-old coal-miner who had thrombocytopenia and appearance of erythroblasts in peripheral blood preceding the development of chronic myelogenous leukemia was reported. Although his clinical course resembled that of acute leukemia, examination of the blood and bone marrow films always showed the characteristic findings of chronic myelogenous leukemia. Some aspects of these atypical clinical manifestations and course were discussed.View full abstractDownload PDF (588K)
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