All issues
Volume 17, Issue 12
Displaying 1-9 of 9 articles from this issue
- |<
- <
- 1
- >
- >|
-
[in Japanese]1976 Volume 17 Issue 12 Pages 1235-1260
Published: 1976
Released on J-STAGE: January 19, 2009
JOURNAL RESTRICTED ACCESSDownload PDF (1934K)
-
Mineya TANAKA, Masao TANAKA, Yasumitsu NAKAIDE, Morimasa HASEBE, Yuji ...1976 Volume 17 Issue 12 Pages 1261-1265
Published: 1976
Released on J-STAGE: January 19, 2009
JOURNAL RESTRICTED ACCESSTreatment with Vincristine was assessed in eleven patients with idiopathic thrombocytopenic purpura. Nine of them had previously been refractory to glucocorticoid, and two of which had also been refractory to immunosuppressants. Vincristine was given intravenously in a dose of 1 or 2 mg every seventh or tenth day. There were two good, three fair and six poor results. Two good responders were given 2 mg Vincristine per week. In other nine patients, who were given 1 mg Vincristine every week, three responded fairly well and six responded poorly. Platelet count increased in a week after Vincristine injection in a 1 mg dose or 2 mg dose, and more increase was observed in a 2 mg dose. Two good responders complained of severe peripheral neuropathy, and therefore Vincristine had to be stopped. Platelet count in these patients fell soon after the discontinuation of Vincristine.
Our result of treatment with Vincristine in idiopathic thrombocytopenic purpura has been poor as compared with the report described by Ahn et al, and may be worse than that of other immunosuppressants. However, platelet response is more prompt with Vincristine than with other immunosuppressants. Further investigations are required to evaluate Vincristine in the treatment of idiopathic thrombocytopenic purpura.View full abstractDownload PDF (371K) -
I. A New Histochemical Method to Detect NBT Reduction by NeutrophilsMasao TOMONAGA1976 Volume 17 Issue 12 Pages 1266-1275
Published: 1976
Released on J-STAGE: January 19, 2009
JOURNAL RESTRICTED ACCESSNBT test, introduced by Park et al in 1968, has been widely used as a rapid and simple aid to detect chronic granulomatous disease (CGD) and systemic bacterial infections. However, technical problems chiefly due to NBT-heparin complex were not solved. Recently the author found that NBT dye itself had anticoagulant activity and was able to establish a new histochemical method without using heparin.
In this semi-quantitative method neutrophils are classified into five types (O, I, II, III and IV) on the basis of the pattern of formazan deposit in the cytoplasm. Cells of each type are given a square of the type number for their degree of positivity. (O=0, I=1, II=22 (4), III=32 (9), IV=42 (16)) The NBT score is defined as the sum of the degree of 100 cells and NBT rate as the percentage of positive cells.
Normal controls showed mean NBT score 101.5 (O-2.5%, I-96.3%, II-1.1%, III-0.1%, IV-0%) and mean NBT rate 96.5%.
Most patients having systemic bacterial infections showed elevated scores over 200 and positive cells of III and IV were increased in number. Patients having non-bacterial diseases did not show high score except two cases of SLE with slightly elevated scores.
In two cases of CGD, normal scores were obtained even in severe pseudomonas infection. Their neutrophils, however, completely failed to respond to endotoxin and scores remained within normal range.
Principles of the method and technical procedures are chiefly mentioned in this report.View full abstractDownload PDF (879K) -
II. Effect of Adherent Cell on in Vitro Colony Formation in Children with Aplastic AnemiaTatsutoshi NAKAHATA, Hirokazu NISHIHIRA, Yasunobu WAKABAYASHI, Taro AK ...1976 Volume 17 Issue 12 Pages 1276-1282
Published: 1976
Released on J-STAGE: January 19, 2009
JOURNAL RESTRICTED ACCESSColony stimulating or inhibiting functions of the adherent cells in the bone marrow of children with aplastic anemia were studied. Utilizing the adherent cell separation technique, the marrow cells from 6 children with aplastic anemia and 6 children with non-hematological diseases (controls) were separated into non-adherent cells (NA cells) and adherent cells (Ad cells). The culture was carried out according to the method of Robinson and Pike. The colony forming efficiency of the unseparated marrow cells and of the NA cells was compared. Mixed cultures of the unseparated marrow cells obtained from the patients with aplastic anemia and those from the control were performed. The functions of Ad cells were assessed by influence on colony formation of the NA cells in the mixed culture.
The results obtained were as follows:
1) In the controls, the colony counts of the unseparated cells and NA cells ranged from 106 to 244, from 86 to 313, respectively.
2) The patients with aplastic anemia showed remarkablly low colony formation by the both marrow cells: unseparated cells formed from 0 to 10 colonies and NA cells formed from 0 to 16 colonies.
3) When the marrow cells from the patients and controls were mixed, the colony counts reduced approximately to one fourth of the control marrow cells.
4) The Ad cells of the control subject stimulated colony formation of the NA cells of the control, while those from the patients with aplastic anemia inhibited colony formation of the NA cells of the control.
5) The Ad cells of the control stimulated a little colony formation of the NA cells from the patients, while those from the patients failed to stimulate colony formation. These results suggest that the defective function of the Ad cells in bone marrow may be a cause of secondary decrease of CFC.View full abstractDownload PDF (561K)
-
Yasuyuki ENDO, Akira MIURA, Akira SHIBATA, Goro KAKIZAKI, Tsutomu WATA ...1976 Volume 17 Issue 12 Pages 1283-1288
Published: 1976
Released on J-STAGE: January 19, 2009
JOURNAL RESTRICTED ACCESSThe patient, a 62 year old housewife, was referred here from a certain clinic because of the chief complaint of hepatosplenomegaly. Hematologic examination revealed a moderate degree of anemia with associated leukoerythroblastosis and increased WBC. The neutrophils showed a high alkaline phosphatase score and no abnormal (Ph1) chromosome was demonstrable in the peripheral blood cells or bone marrow cells; hence distinguishable from chronic myelogenous leukemia (CML). The patient's peripheral blood showed normal ferrokinetics and a notably shortened red cell life span. The Mantoux test was negative and there existed no abnormal finding as to the blood coagulation and fibrinolytic systems whereas the platelet adhesiveness was noted to be reduced. Bone marrow biopsy disclosed a marked degree of fibrosis and there was evidence of conspicuous extramedullary hematopoiesis in the liver; the case was diagnosed as primary myelofibrosis. The patient was admitted to this hospital for splenectomy since she had failed to respond to prednisolone therapy with any significant amelioration in anemia at the clinic and because of progressively increasing splenomegaly with development of pain. There were abscesses in the spleen and the patient was diagnosed microscopically as splenic tuberculosis. Extramedullary hematopoiesis was also prominent in the spleen. After the operation, serum hepatitis and pneumonia developed and she died of these conditions and incomplete suturation.
While there has been no reported case of “myelofibrosis secondary to tuberculosis” in Japan, a total of 252 cases of myelofibrosis were reported during the period from 1940 to 1975 and as many as 28 cases (11.1%) among them had a complication or a history of tuberculosis. Importance of the study of myelofibrosis in closer connection with tuberculosis has been emphasized.View full abstractDownload PDF (608K) -
Ryuichi TAKAHASHI, Osamu KOIDE1976 Volume 17 Issue 12 Pages 1289-1294
Published: 1976
Released on J-STAGE: January 19, 2009
JOURNAL RESTRICTED ACCESSAn autopsied case of clinically diagnosed as acute myelofibrosis is reported.
A 59-year-old male patient was admitted with anemic symptoms. His blood picture showed pancytopenia, but gradually developed leukoerythroblastic anemia with scattered blasts. Bone marrow aspiration was blood tap and biopsy of the bone marrow showed myelofibrosis, but the spleen was not felt. His course was rapidly fatal, only 6 months.
The post-mortem examination showed myelofibrosis of the bone marrow without extramedullary hematopoiesis and leukemic infiltration.
Reviewing previously reported cases, acute myelofibrosis may require careful differentiation from other myeloproliferative disorders.View full abstractDownload PDF (649K) -
Masao KISHITANI, Ryoyu TAKEDA1976 Volume 17 Issue 12 Pages 1295-1301
Published: 1976
Released on J-STAGE: January 19, 2009
JOURNAL RESTRICTED ACCESSIt has been considered that M-protein has no antibody activities. However, since Badin et al. have reported the first case of M-component with anti-streptolysin-O (ASLO) activity, a number of M-components exhibiting various antibody activities have been documented.
The immunoglobulin of all the reported cases so far was confined to IgG, and it was found that the antibody activity located on the Fab fragment. The present paper deals with the first case of IgA myeloma with ASLO activity.
In this case, it was proved that ASLO activity was present in the fractions corresponding to M-component in agar gel electrophoresis. Because of technical complexity in separating and refining the IgA, the pattern of ASLO activity detected by Rantz-Randall's method in each fractional eluate from electrophoresis of the patients sera was compared to that of IgA concentration in the corresponding fraction. As the result, peak location of IgA concentration coincided with that of ASLO activity.
The problem is whether ASLO activity in M-component represents a true antibody activity. In the present case ASLO activity was of abnormally high titer in the Rantz-Randall's method, but it was normal in the passive hemagglutination method. Moreover, we failed to demonstrate that ASLO activity represents true antibody itself, that is, serum of our case did not show precipitation reaction with SLO and not inhibit precipitation reaction between SLO and serum of patient with streptococcus infection.View full abstractDownload PDF (644K) -
Seiji FURUYA, Shin-ichi MIYAMOTO, Yoshiaki KUMAMOTO1976 Volume 17 Issue 12 Pages 1302-1308
Published: 1976
Released on J-STAGE: January 19, 2009
JOURNAL RESTRICTED ACCESSA 56-year old woman with vesicosigmoidal fistula had a history of previous blood transfusion more than four times. She received 600 ml of blood transfusion during the procedure of ileal conduit. Four days after operation, 200 ml of blood was transfused because of anemia, followed by shivering about one hour later. On the next day, she had jaundice and fever. All blood was found compatible by bromelin method before operation.
Serological studies revealed a secondary response of hemolytic transfusion reaction due to anti-e antibody. The patient's blood type was B, Ms, ccDEE, kk, Fy (a+b-), Dia-, Lua+, Kp (a-b+).
Jaundice and fever disappeared by treatment with hydrocortisone and predonisolone 2 weeks later.View full abstractDownload PDF (532K) -
Yuhko UYAMA, Mitsuo MIZUI, Hiroshi TANAKA, Tsuneo NINOMIYA, Tadashi YA ...1976 Volume 17 Issue 12 Pages 1309-1319
Published: 1976
Released on J-STAGE: January 19, 2009
JOURNAL RESTRICTED ACCESSThis report concerns with cyclic neutropenia in a 7-year-3-month-old girl, having stomatitis, furunculosis, lymphadenitis and fever up to 39°C in the interval of approximately 3 weeks since the age of 2 weeks.
The peripheral blood picture after admission revealed that the neutropenia recurred every 19 days and the other formed elements of the blood, monocytes, lymphocytes, eosinophils, basophils and red blood cells, also showed periodic fluctuations. During the neutropenic phase, bone marrow was hypocellular with absence of mature neutrophils and increase in neutrophilic promyelocytes. During the neutrophilic phase, bone marrow was normocellular with increase in mature neutrophils, neutrophilic myelocytes and metamyelocytes and decrease in neutrophilic promyelocytes and myeloblasts. The serum protein electrophoresis showed hypergammaglobulinemia. Bone marrow granulocyte reserves to hydrocortisone and marginal granulocyte reserves to epinephrine were tested during both neutropenic and neutrophilic phases. It was concluded that bone marrow and marginal granulocyte pool sizes were generally small during the neutropenic phase. When no netrophils were present in the peripheral blood, local inflammatory response (skin window test) was hypocellular with mononuclear cell infiltrations 10 hours after abrasion. When netrophils were normal in number, abundant neutrophilic infiltration began 2 hours after the initiation of inflammation, and neutrophilic infiltration was gradually replaced by mononuclear cell infiltration beginning after 5 hours.View full abstractDownload PDF (1087K)
- |<
- <
- 1
- >
- >|