All issues
Volume 13 (1972)
- Issue 6 Pages 923-
- Issue 5 Pages 769-
- Issue 4 Pages 455-
- Issue 3 Pages 249-
- Issue 2 Pages 105-
- Issue 1 Pages 1-
Volume 13, Issue 3
Displaying 1-28 of 28 articles from this issue
- |<
- <
- 1
- >
- >|
-
[in Japanese]1972 Volume 13 Issue 3 Pages 249-254
Published: 1972
Released on J-STAGE: October 31, 2008
JOURNAL RESTRICTED ACCESSDownload PDF (488K)
-
Leon DMOCHOWSKI1972 Volume 13 Issue 3 Pages 255-256
Published: 1972
Released on J-STAGE: October 31, 2008
JOURNAL RESTRICTED ACCESSDownload PDF (164K)
-
1972 Volume 13 Issue 3 Pages 257-259
Published: 1972
Released on J-STAGE: October 31, 2008
JOURNAL RESTRICTED ACCESSDownload PDF (201K)
-
Satoshi INAGAKI1972 Volume 13 Issue 3 Pages 260-265
Published: 1972
Released on J-STAGE: October 31, 2008
JOURNAL RESTRICTED ACCESSOn the observation of basophils in blood and bone marrow smears by using basic dyes, two phenomena were found and were applied in the several staining methods.
1) When fresh air-dried smears were directly dipped in the suitable high concentrated basic dye solution in methanol for the short time, the granules of basophils could be fixed and be stained concurrently (Simple staining method). Applicable dyes: Methylene blue, New methylene blue N, Toluidine blue O, Azure A and Safranin O.
2) When the suitable high concentrated basic dye solution in methanol were used for the fixation followed by the staining with aqueous basic dye solution in fresh air-dried smears, the replacement phenomen occured between the two basic dyes easily and the granules of basophils were stained securely with the latter (Replacement staining method). It was the indispensable condition that the main component of the solvent for the fixation dye is methanol and that of the staining dye is water. The dyes which could not be used in the simple staining method for the several unsuitable conditions were applied in this method. Applicable dyes: a. Fixation dyes (the dyes used in the simple staining method and Acridine orange). b. Staining dyes (Azure B, Brilliant crecyl blue, Thionin, Astra blue, Bismarck brown and etc.).
These phenomena were also common to the constituents of blood cells with the affinity to the basic dyes.
The following methods were found in the consideration for the conditions of solvents and others.
1. Astra blue (AB) staining (AB-Kernechtrot, AB-Safranin O, AB-Giemsa, AB-Periodic acid-Schiff staining).
2. Bismarck brown-Hematoxylin staining.
3. Acridine orange-Giemsa staining. (The staining mechanism of Giemsa and May-Grünwald-Giemsa staining were made clear by applying the above two phenomena.)
Using the above methods, it was considered that the clinical significances of the morphology on the basophil would be able to clear.View full abstractDownload PDF (481K) -
Yukio UEDA, Junsuke YOSHITAKE, Toru MASAOKA1972 Volume 13 Issue 3 Pages 266-272
Published: 1972
Released on J-STAGE: October 31, 2008
JOURNAL RESTRICTED ACCESSThe study aims to clarify the clinical significance of blood basophils. At the onset of the study, a quanitative analysis of the basophils were performed.
The cells decrease in their number at thirties of age and then increase gradually as human grows on. The motile type of the cells was divided into 3 types. The velocity of the movement is slower than that of neutrophils and eosinophils but faster than lymphocytes and monocytes. No chemoaxis to staphylococcus aureus is defected.
Change in number of the cells in various leukemias was determined, finding that in acute leukemia the number increases as a reflective of an improvement of hematopoiesis in bone marrow, but in chronic leukemia after growing worse in the clinical condition arises their increase.
In short alteration of the blood basophils is taken to be a reflection of effect of therapy in various leukemia.
Our direct method using Haemaccel is recommended to count the blood basophils in leukemia especially in acute leukemia.View full abstractDownload PDF (505K) -
Ikuro KIMURA1972 Volume 13 Issue 3 Pages 273-280
Published: 1972
Released on J-STAGE: October 31, 2008
JOURNAL RESTRICTED ACCESSWe have investigated the morphological and functional changes of basophilic leukocytes seen in the patients with blood dyscrasias and allergic disorders, and have obtained the following results.
An acute exacerbation seen in the patients with chronic myeloid leukemia can be indicated by the changes in basophilogram. Here, we grade the basophilogram according to the numbers of granules in basophilic leukocytes.
Changes in basophils are closely related to allergic reaction, for instance, asthmatic attack especially of the reagin type. No quantitative relationship was noticed between the numbers of basophils and amounts of IgE.
Specific immunological bindings of basophils to IgE can be seen most conspicuously in the cases of bronchial asthma, whereas these bindings are far less marked in healthy individuals and very weak in chronic myeloid leukemia patients.
Basophils can be found at the very site of the reagin type allergic reactions. This is well demonstrated in skin vesicle tests in which one can see numerous accumulated basophils.
Heparin contained in basophils will suppress the reagin type reaction. Thus, the basophils may control provocations of as well as reparings of allergic reaction.View full abstractDownload PDF (738K) -
Satoshi YOSHIKAWA1972 Volume 13 Issue 3 Pages 281-288
Published: 1972
Released on J-STAGE: October 31, 2008
JOURNAL RESTRICTED ACCESS(I) The changes in the number of basophilic granulocytes in ear-venous blood of rabbits after injection of bovine serum albumin (BSA) to male rabbits non-treated or sensitized with BSA were studied. The results are as follows.
1. Basophilic granulocytes in the rabbits to which BSA were intravenously or subcutaneously injected once (the first sensitization) increased in number from +200% to +500% at 30 or 60 minutes later.
2. From 15 to 30 minutes after injection of BSA in the rabbits previously sensitized with BSA, the counts of the basophilic granulocytes dropped to nearly -100%, then increased in number.
3. A decrease of basophilic granulocytes caused by antigen-antibody reaction with BSA was also observed in vitro.
(II) 1032 patients which in our hospital were examined peripheral basophilic granulocytes by the direct method.
1. Patients The number of pasophilic grannlocyes in given steroid hormone were below normal range.
2. In the cases with eosinophilic granulocytosis or appearance of atypical lymph in the peripheral blood, many basophilic granulocytosis were seen.
3. In the patients with bronchial asthma, tuberculosis and usual infectious disorders, basophilic granulocytes frequently increased in number.
On the other hand, in the patients with collagen diseases, a significant decrease of basophilic granulocytes was observed.
4. The marked basophilic granulocytosis was seen for 100 to 120 days in the postsplenectomized patients with idiopathic thrombocytopenic purpura.
The findings of the basophilic granulocytosis or granulocytopenia mentioned above were discussed in relation to immune reactions.View full abstractDownload PDF (565K) -
[in Japanese]1972 Volume 13 Issue 3 Pages 289-295
Published: 1972
Released on J-STAGE: October 31, 2008
JOURNAL RESTRICTED ACCESSDownload PDF (543K) -
[in Japanese], [in Japanese]1972 Volume 13 Issue 3 Pages 296-297
Published: 1972
Released on J-STAGE: October 31, 2008
JOURNAL RESTRICTED ACCESSDownload PDF (154K)
-
[in Japanese]1972 Volume 13 Issue 3 Pages 298-305
Published: 1972
Released on J-STAGE: October 31, 2008
JOURNAL RESTRICTED ACCESSDownload PDF (745K) -
[in Japanese]1972 Volume 13 Issue 3 Pages 306-312
Published: 1972
Released on J-STAGE: October 31, 2008
JOURNAL RESTRICTED ACCESSDownload PDF (571K) -
With Special Reference to Retro- and Pro-Spective AnalysisNanao KAMADA, Haruto UCHINO1972 Volume 13 Issue 3 Pages 313-322
Published: 1972
Released on J-STAGE: October 31, 2008
JOURNAL RESTRICTED ACCESSHematologic and cytogenetic studies were made on five cases of leukemia patients who had history of atomic bomb exposure and blood disorders such as anemia and/ or leukopenia for 5 or 13 years preceding the terminal development of acute leukemia (The first group). Of the 5 cases, 3 were exposed within 1,000 meters from the hypocentre. The type of leukemia in these patients were 3 of erythroleukemia, 2 of acute monocytic leukemia. Hematologically abnormal characteristics in the preleukemic stage in these cases were anemia (all cases, 5/5), monocytosis (4/5), prolonged leukopenia for over 10 years (4/5), appearance of erythroblasts in the blood one to 5 years prior to the development of leukemia (4/5), erythroid hyperplasia in bone marrow (3/5), maturation arrest in the granulocyte series (3/5) and presence of abnormal mitosis resulting in bi-nucleated immature granulocytes in bone marrow and its maturation forms in blood (3/5). Chromosome aberrations were observed in 4 of the 5 cases in the period before their leukemia became overt clinically.
There were other 5 cases with anemia (sideroblastic, PNH and aplastic) which had chromosome aberrations in the bone marrow cells but were not being diagnosed as leukemia yet (The second group). Cytological studies revealed the same kind of abnormalities including bi-nucleated immature granulocyte in the patient with sideroblastic anemia but not in PNH and aplastic anemia.
Furthermore, five heavilly irradiated persons were presented who had abnormal chromosomes with some clones in their marrow cells without any clinical complaints and signs (The third group).
Morphologically abnormal characteristics, especially presence of bi-nucleated immature granulocyte, and bone marrow chromosome aberrations in the preleukemic stage was discussed in terms of leukemogenesis following radiation exposure.View full abstractDownload PDF (1304K) -
Tetsuro SIMAMINE1972 Volume 13 Issue 3 Pages 323-330
Published: 1972
Released on J-STAGE: October 31, 2008
JOURNAL RESTRICTED ACCESSHistological examinations of serial biopsies of bone marrow and autopsy materials were performed on cases of myeloid leukemia which had preleukemic state or atypical early manifestations.
Structural changes of bone marrow in so-called preleukemic state were classified in,
a. hypoplastic type
b. erythroproliferative type
c. granuloproliferative type
d. myeloproliferative type.
Characteristics of these types in their further course and autopsy findings were described. It was stressed, that myeloid leukemia with preleukemic state often showed also atypical autopsy findings, compared with myeloid leukemia without preleukemic state.View full abstractDownload PDF (643K) -
[in Japanese]1972 Volume 13 Issue 3 Pages 331-333
Published: 1972
Released on J-STAGE: October 31, 2008
JOURNAL RESTRICTED ACCESSDownload PDF (219K) -
[in Japanese]1972 Volume 13 Issue 3 Pages 333-335
Published: 1972
Released on J-STAGE: October 31, 2008
JOURNAL RESTRICTED ACCESSDownload PDF (227K) -
Michito ICHIMARU1972 Volume 13 Issue 3 Pages 335-337
Published: 1972
Released on J-STAGE: October 31, 2008
JOURNAL RESTRICTED ACCESSReview of the data accumulated at Nagasaki University and ABCC during the past 20 years revealed 156 cases of aplastic anemia.
The risk of aplastic anemia among Atomic-bomb survivors did not show statisticaly significant increase.
2 cases of acute leukemia among proximally exposed survivors showed temporal severe decrease of leucocyte count immediately following Atomic-bomb explosion.
8 cases of specific anemia among proximally exposed persons during about 1949∼1955 were discovered. These cases showed pancytopenia of peripheral blood and blast cell focus in some cases or acute myelofibrosis in other cases in bone marrow.
It seems that the bone marrow of Atomic-bomb survivors who was exposed to heavy dosis of radiation was one of preleukemic status.View full abstractDownload PDF (239K) -
[in Japanese]1972 Volume 13 Issue 3 Pages 338-339
Published: 1972
Released on J-STAGE: October 31, 2008
JOURNAL RESTRICTED ACCESSDownload PDF (196K) -
[in Japanese]1972 Volume 13 Issue 3 Pages 339-341
Published: 1972
Released on J-STAGE: October 31, 2008
JOURNAL RESTRICTED ACCESSDownload PDF (230K)
-
Takaaki FUJII1972 Volume 13 Issue 3 Pages 342-354
Published: 1972
Released on J-STAGE: October 31, 2008
JOURNAL RESTRICTED ACCESSDownload PDF (1184K)
-
Masao OGURO1972 Volume 13 Issue 3 Pages 355-362
Published: 1972
Released on J-STAGE: October 31, 2008
JOURNAL RESTRICTED ACCESSAs far as PAS positivity in erythroblasts is concerend, it has been generally found to be negative. In 1960, however, Quaglino reported that erythroblast in some hematological disorder show PAS positive and pointed out the diagnostic evaluation for di Guglielmo syndrome. This investigation purposed to clear PAS positivity in erythroblast on different pathological condition and its clinical evaluation.
PAS reactions which obtained different method carried out on the bone marrow smears of 108 cases with various disease including a case of normal adult and human fetal erythroblast.
For frequency of PAS positive erythroblasts, 100 erythroblasts of each specimen were observed and percent positivity were counted. In all specimens, the percent positivity showed from 4 to 90% and some conclusions were obtained as follows;
1) It seemed that PAS reactive erythroblasts have no correlation with the erythroid proliferaiton and occur in all specimens.
2) As with PAS positive lymphocytes, the percent positivity of PAS positive erythroblasts revealed a different value depend on a method (various temperature and pH for reaction) of PAS reaction.
3) Although the percent positivity of PAS positive erythroblast showed high frequence on erythroleukemia, acute myelocytic leukemia and suspected di Guglielmo syndrome, percent positivity of PAS positive erythroblast may not be valuable index for diagnosis of special disease such as malignant erythroid disturbance.View full abstractDownload PDF (835K) -
Shimpei NAKAZAWA, Takashi MEGURO, Zenzaburo YAMADA, Kaneo YAMADA1972 Volume 13 Issue 3 Pages 363-369
Published: 1972
Released on J-STAGE: October 31, 2008
JOURNAL RESTRICTED ACCESSStaphylococcal clumping test (SCT) is the new method in measuring the levels of fibrinogen and fibrin degradation products (FDP). This method was reviewed by Hawiger and his co-workers in 1970.
The purpose of our study was to evaluate the practical method and clinical use of SCT.
Our conclusions are follows:
1) SCT was simple and rapid method for detecting FDP.
2) The titer of the staphylococcal clumping was stable and did not changed by long term preservation. Very slight difference of titers was observed in the different lots. According to this result, it was considered that the clumping titer of each lot should be examined by the standard fibrinogen solution.
3) In vitro studies revealed that the high molecular FDP were selectively responsible for SCT. FDP solution were made by incubation with plasmin and purified fibrinogen, and the high molecular FDP were obtained by the method of Sephadex G-200, and DEAE Sephadex A-50 column chromatography.
4) In infants, children, and adults, the FDP levels measured by SCT showed almost same levels which were measued by hemagglutination inhibition test (HIT).
5) In cord bloods and newborns, the FDP levels measued by SCT were excessively high in comparison with those measured by HIT.View full abstractDownload PDF (572K) -
Takeo KUROYANAGI1972 Volume 13 Issue 3 Pages 370-377
Published: 1972
Released on J-STAGE: October 31, 2008
JOURNAL RESTRICTED ACCESSStudies on the mechanism of acute anemic crisis in autoimmune hemolytic anemia (AIHA) demonstrated two kinds of crisis, acute hemolytic and acute aplastic. Erythroblasts in the bone marrow decreased slightly in acute aplastic crisis, but they remained with in normal limits. Circulating reticulocytes decreased markedly in spite of the slight reduction of erythroblasts. This suggests a marked increase of ineffective erythropoiesis in acute aplastic crisis.
The erythropoietic efficiency of bone marrow was determined on 7 patients with AIHA by modified Haurani's method. It was within normal limits in two of 7 cases. It decreased in 5 cases, indicating the increased ineffective erythropoiesis in these cases.
Electronmicroscopic examinations on bone marrows of patients with AIHA demonstrated frequent phagocytosis of erythroblasts and red cells by reticulum cells.
The antigen antibody reaction between incomplete autoantibodies of AIHA and bone marrow cells was confirmed by the indirect anti-globulin comsumption test.
These results suggest that an increased abortion rate of erythroblasts caused by incomplete autoantibodies has great significance in the mechanism of the increased ineffective erythropoisis in AIHA.View full abstractDownload PDF (777K) -
K. ISHIZU, E. KAKISHITA, K. NAGAI1972 Volume 13 Issue 3 Pages 378-386
Published: 1972
Released on J-STAGE: October 31, 2008
JOURNAL RESTRICTED ACCESSThe dynamics of HbF-containing erythroblasts in hereditary spherocytosis was studied before and after splenectomy. The analysis was performed by employing a classification of erythroblasts based on their cell cycle as well as by use of the fluorescent antibody technique.
Results obtained were as follows:
1) Before splenectomy, a marked erythroid hyperplasia with early denucleation of HbF-containing erythroblasts was observed in bone marrow and large numbers of stress reticulocytes appeared in peripheral blood.
2) The erythroid hyperplasia in bone marrow and reticulocytosis in circulating blood were normalized after splenectomy.
3) Influences of splenectomy on HbF-containing cell line were the decrease in precursor erythroblasts and the increase in later erythroblasts. This results in a temporary increase of HbF-containing erythrocytes in peripheral blood.
From these findings, it is proposed that splenectomy causes the diminution of red cell destruction and suppresses the overproduction of erythropoietin. The decrease in erythropoietin or the improvement of tissue hypoxia may inhibit the differentiation of stem cells to HbF-containing proerythroblasts and may promote the further differentiation of later erythroblasts having HbF, without early denucleation. Moreover, these evidences may be important in clarifying the hematological responses to splenectomy in hypersplenism.View full abstractDownload PDF (729K)
-
Hiroko HIRAI, Hisashi KAWAGUCHI, Fumio KUMA1972 Volume 13 Issue 3 Pages 387-391
Published: 1972
Released on J-STAGE: October 31, 2008
JOURNAL RESTRICTED ACCESSReported was sulfhemoglobinemia which developed in a 53-year-old female. After having taken a herb medicine containing acetanilid and precipitated sulfur for 3 years, the patient developed cyanosis and anemia which were confirmed by spectroscopy to be attributable to an increase in sulfhemoglobin. Measurement by Evelyn-Mallory method revealed the methemoglobin to be nearly normal (1.55%) but the sulfhemoglobin to be remarkably increased (20.1%).
Since there was no history of constipation for the onset of the present disease, it was thought that the long-term administration of precipitated sulfur together with acetanilid was responsible. The initial examination showed a high rate of Heinz bodies, reticulocytosis, disappearance of haptoglobin and erythroid hyperplasia of the bone marrow, leading to the presumption of the involvement of the hemolytic process due to the drug.
Although reduced enzyme activity within the erythrocytes has been referred to as the intrinsic mechanism for the onset of the present disease, there was no abnormality in the present case as far as examined.View full abstractDownload PDF (388K) -
Yoshio MIZUNO, Hiroe NAKAZAWA, Hirokuni SAKAGUCHI, Yoshiyuki HONDA, Ke ...1972 Volume 13 Issue 3 Pages 392-396
Published: 1972
Released on J-STAGE: October 31, 2008
JOURNAL RESTRICTED ACCESSA 69 year-old female having a history of longstanding constipation had taken “Nosin” containing acetanilid because of headache for 10 years. She had noted angina-like precordial pain for 5 years. Three years prior to admission cyanosis in her face developed. Blood examination on admission showed RBC 286×104, reticulocytes 32‰, WBC 4500 with a normal differential and erythroid hyperplasia of the bone marrow.
There was a spectrophotometric absorption at 617 millimicron in hemolysate which did not change by an addition of potassium cyanide, suggesting that she had sulfhemoglobinemia. Sulfhemoglobin content was 1.19 gm/dl (14.2%) whereas methemoglobin was 0.23 gm/dl. Three months after discontinuation of acetanilid, precordial pain, cyanosis and anemia disappeared and the spectrophotometric absorption of sulfhemoglobin was no more detected.
Eleven cases of sulfhemoglobinemia previously reported in Japan were briefly reviewed in comparison with the present case.View full abstractDownload PDF (378K) -
T. MAEKAWA, H. ISHIKAWA, M. AZUMA, S. SAWATARI, I. IMAI, K. OKA, Y. IM ...1972 Volume 13 Issue 3 Pages 397-403
Published: 1972
Released on J-STAGE: October 31, 2008
JOURNAL RESTRICTED ACCESSThe clinical course and autopsy findings of a male patient, 51 years of age, with multiple myeloma of an atypical type were reported. A number of myeloma cells were observed in smears of bone marrow aspirate from both the sternum and the right iliac crest. X-ray films of the skull and other bones revealed many punched-out areas. Serum total protein was about 5 g/dl and no proteinuria was detected throughout his clinical course. No increase was observed in any of serum globulin fractions. Concentration of serum gamma-globulin fraction was less than 2 per cent of the total protein. M-bow formation was not observed in immunoelectrophoretic analysis of either the patient's serum or urine against anti-IgG, -IgA, -IgM, -IgD, -IgE and-light chain of both κ and λ sera. Incorporation rate of 75Se-methionine into the gamma-globulin fraction was low and delayed as compared with those of normal persons and of several patients with typical IgG-myeloma. He died of cholestatic jaundice due to myelomatous lesions in the gallbladder and adjacent lymph nodes, and congestive heart failure. Autopsy revealed a number of myeloma nodules in the bone marrow, liver, spleen, pancreas, gallbladder, kidneys and lymph nodes. In addition, numerous miliary to pea-sized lesions of tuberculosis were observed in the lungs.View full abstractDownload PDF (784K) -
Ryo KOIDE, Kiyoko KIKUCHI, Yukiko TSUNEMATSU, Takashi NAKAJIMA1972 Volume 13 Issue 3 Pages 404-410
Published: 1972
Released on J-STAGE: October 31, 2008
JOURNAL RESTRICTED ACCESSRecently we were visited by a 34-month-old boy who was suffering from megaloblastic anemia.
He was pale and had lost his appetite but he had no jaundice, no bleeding tendency and no diarrhea. His development was almost within normal limits.
His parents are consanguineous, but no remarkable disease related was found in their families and siblings.
Diagnosis of megaloblastic anemia, caused by vitamine B12 deficiency, was made by high urine MMA value, low serum vitamin B12 value and a remarkable increase of reticulocytes following parenteral administration of vitamine B12.
Histological examination revealed no abnormality in the biopsy specimens of the stomach, jejunum and ileum.
Gastric juice was normal, containing the intrinsic factor. No antibodies against intrinsic factor and parietal cell antibodies were found in the serum.
Schilling tests were performed with or without intrinsic factor during the period of anemia and at the time of remission. Besides, it was done by the administration of the normal duodenal juice of adult. The tests proved to be negative in each of the five examinations.
The endocrinological function tests, and absorption tests for sugar, amino acid and fat were normal.
No abnormality was found in the renal function test, except positive protein in the urine.
The present case is thought to represent the selective vitamine B12 malabsorption with benign proteinuria, and the first case reported in Japan.View full abstractDownload PDF (629K)
-
[in Japanese], [in Japanese], [in Japanese], [in Japanese]1972 Volume 13 Issue 3 Pages 411-414
Published: 1972
Released on J-STAGE: October 31, 2008
JOURNAL RESTRICTED ACCESSDownload PDF (378K)
- |<
- <
- 1
- >
- >|