This research focuses on the method for predicting the fatigue life of the F type pole of the highway road variable message signs by measuring acceleration. Fatigue fracture is one of the aging degradation concerns for ancillary facilities such as variable message signs and lighting installed on highways. The relationship between the fatigue life and the applied stress has been established by the S-N curve, and the fatigue life is predictable from the hysteresis of stress amplitude. By the proposed method, the strain at the area of concern for fatigue damage is estimated from the acceleration and the cumulative damage level is evaluated from the minor law. In this paper, the validity of the strain estimation method from the acceleration measurement is verified and the long-term stability of the method is evaluated from the measurement results of several actual structures. The effectiveness and prediction accuracy of the proposed method are also verified by evaluating the cumulative damage from the same data. The measurement period required for stable evaluation of fatigue life is also discussed.