SEIBUTSU BUTSURI KAGAKU

Use of α-fetoprotein for diagnosis and therapy of hepatoma

Hidematsu Hirai

1984 Volume 28 Issue 6 Pages 333-341
Published: December 25, 1984
Released on J-STAGE: March 31, 2009

DOIhttps://doi.org/10.2198/sbk.28.333

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Workshop

1984 Volume 28 Issue 6 Pages 343-385
Published: December 25, 1984
Released on J-STAGE: March 31, 2009

DOIhttps://doi.org/10.2198/sbk.28.343

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Wheat amylase inhibitor and its action on amylase activity

Itsuo Suehiro, Makoto Otsuki, Toru Oka, Atsushi Ohki, Yoshinori Okabay ...

1984 Volume 28 Issue 6 Pages 387-391
Published: December 25, 1984
Released on J-STAGE: March 31, 2009

DOIhttps://doi.org/10.2198/sbk.28.387

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Effect of wheat amylase inhibitor (Isoamylase test, Phadebus-Shionogi) on human pancreatic and salivary amylase activities were investigated. Column chromatographic investigations suggested the formation of an amylase-inhibitor complex, which easily dissociated into each component after electrophoresis and the amylase activity was fully recovered. Using the specific inhibitory effect of the inhibitor on salivary amylase activity, the ratio of activity of pancreatic to salivary amylase (P/S ratio) in serum was determined. P/S ratio of serum from patients with acute pancreatitis was more than 8.0 whereas that of serum from patients with mumps, with lung disease or postoperative hyperamylasemia associated with salivary-type hyperamylasemia was less than the normal value. However, hyperamylasemia due to macroamylasemia or renal failure could not be discriminated by this method, because the P/S ratio in these patients was the same as the normal value.

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Carrier free isoelectric focusing of proteins in the presence of detergents

Kaoru Yasukawa, Kiyotsugu Kojima, Takashi Manabe, Tsuneo Okuyama

1984 Volume 28 Issue 6 Pages 393-400
Published: December 25, 1984
Released on J-STAGE: March 31, 2009

DOIhttps://doi.org/10.2198/sbk.28.393

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We studied on the effect of various detergents on the pH gradient formed by Ampholine and also on the effect on migration rate of soluble proteins in the presence of detergents.
Nonionic detergents (Emulgen 108, Triton X-100, Brij 35) affected scarcely the pH gradient formation, and the migration rate of proteins was affected but not remarkably. As SDS (anionic detergent) concentration increased, the slope of the pH gradient decreased and the protein peak was transported cathodically.

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Physical and Functional Heterogeneities of Lectin Preparations as Revealed by Electrophoretic Techniques

Kazuhisa Taketa, Masaki Izumi

1984 Volume 28 Issue 6 Pages 401-408
Published: December 25, 1984
Released on J-STAGE: March 31, 2009

DOIhttps://doi.org/10.2198/sbk.28.401

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Upon electrophoresis of Con-A, LCA-A, LCA-B and LCA at pH8.6, minor anodic components, which had no affinity for lectin-reactive AFP, were found in some lectin preparations. Time-dependent increase in electrophoretic mobility, spreading of electrophoretic band and loss of affinity for AFP were observed particularly with E-PHA when the lectin was dissolved in buffer at pH8.6 and kept at 4°C. These changes of E-PHA and the formation of minor bands of other lectins were reduced by lowering the pH to 7.5 and minimized by the presence as low as 0.1mM each of MnCl₂, CaCl₂ and MgCl₂. LCA preparations were relatively stable at pH8.6 without added divalent cations. Lot to lot variations in electrophoretic mobility and affinity for AFP were found with LCA-B. Heterogeneity of subunit composition was also present in some lectins. Subunit molecular weights for the common and major component for each lectin, determined in the present study, were 27, 500 for Con-A, 19, 500 for LCA-A and 30, 000 for E-PHA. Lectin-reactive AFP was suggested as a simple and sensitive measure for quality control of lectins. Conversely, those variabilities of lectins should be taken into account in lectin-affinity electrophoresis of glycoproteins.

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Classification and symbolic representation of LDH isozyme anomaly patterns due to LDH linked immunoglobulins

Tatsuo Tozawa

1984 Volume 28 Issue 6 Pages 409-413
Published: December 25, 1984
Released on J-STAGE: March 31, 2009

DOIhttps://doi.org/10.2198/sbk.28.409

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Classification and symbolic representation of LDH isozyme anomaly patterns facilitated to study LDH linked immunoglobulins. A close relation existed between isotypes of LDH linked immunoglobulins and LDH isozyme anomaly patterns on cellulose acetate plate. 31 different patterns were observed in the sera of 112 patients and those were divided into two groups. 27 patterns which were characterized by the anomaly of LDH-5 were in the first group and the others characterized by normal LDH-5 were in the second group. All of the LDH linked IgG's (76 cases), IgA-λ's(3), IgM's(2), IgGA-λ's(2) and IgAM-κ(1) were in the first group, and these patterns were named IgG type. While, LDH linked IgA-κ's(22), IgA-κλ's(2) and IgGA-κ(1) were in the second group, and these patterns were named IgA-κ type. Only 3 cases of LDH liked IgGA-κλ's were in the two types. Two cases (the pair of class and type of immunoglobulins not determinded) were in IgG type, and the other (IgA-κ and IgG-κλ determinded) was in IgA-κ type.

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Isoelectric focusing without carrier ampholytes

Kazuo Shimao

1984 Volume 28 Issue 6 Pages 415-421
Published: December 25, 1984
Released on J-STAGE: March 31, 2009

DOIhttps://doi.org/10.2198/sbk.28.415

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