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Volume 5 (1958)
- Issue 3-4 Pages 83-
- Issue 2 Pages 41-
- Issue 1 Pages 1-
Volume 5, Issue 2
Displaying 1-9 of 9 articles from this issue
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Tokuhiko Higashi, Fukuichi Hasegawa1958 Volume 5 Issue 2 Pages 41-46
Published: November 15, 1958
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSIn order to obtain some additional findings in the research for the formation of plasma proteins, authors have carried out two types of experiments with dogs and rabbits which might be called albuminphersis and globulinpheresis. These have the similar character as plasmapheresis performed by Whipple and others. In alouminpheresis or globulinpheresis, a certain volume of blood was withdrawn from animals, and blood cells were separated from plasma by centrifugation, washed twice with saline, suspended in globulin or albumin solution respectively which had been previously prepared from pooled plasma of the same species, and the resulting mixture, having approximately the same volume and concentration of globulin or albumin as depleted blood, was injected back into amimals intravenously.
By these procedures, animals were brought under the condition in which albumin or globulin fraction was partially lost while other blood components were remained almost unchanged. Changes in serum proteins after these treatments were analyzed at intervals by the micro-Kjeldahl method and the electrophoresis using a Tiselius apparatus.
In this report, it has been shown that the decrease in albumin and the increase in α- and β-globulin have been observed with some differences in details between albumin- and globulinpheresis. Furthermore, on the basis of the results obtained, some discussions have been made about the formation of serum proteins in relation to the labile tissue protein.View full abstractDownload PDF (2832K) -
Kazuo Shimao1958 Volume 5 Issue 2 Pages 47-51
Published: November 15, 1958
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSA new and easy method for the calibration of densitometers was proposed by which linearity of photoelectric current and uniformity either of illumination of slit or photosensitive surface of photocell can be tested. The method consists in scanning of a black paper having a triangular hole (wedge type) with the densitometer to be tested. Two of the densitometers commercially available in Japan were tested by the method. Errors due to nonlinearity of photoelectric current were illustrated.View full abstractDownload PDF (1910K) -
1958 Volume 5 Issue 2 Pages 51
Published: 1958
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSDownload PDF (133K) -
Yoshio Hayashi1958 Volume 5 Issue 2 Pages 52-55
Published: November 15, 1958
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSIn the technique of Paper-electrophoresis, the size of the serum sample is an important factor, because a linear relation between protein concentration and densitometer reading depends upon it considerably.
While the original technique of applying the serum to an electrophoresis paper by a micropipet requires a skill acquired only by repeated practice, the use of an applicator strip which was used in this experiment, makes it easy to apply a constant amount of the serum sample. Furthermore, the above mentioned technique presents a well-separated pattern of serum protein, the separation of α1- and α2-globulin fractions being especially distinct.
The standard errors of the fractions are smaller compared with those by the micropipet method.View full abstractDownload PDF (437K) -
Yoshio Hayashi1958 Volume 5 Issue 2 Pages 56-60
Published: November 15, 1958
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSWhen a measurement of serum protein fractions by the paperelectrophoretic method is made, it is assumed that the amount of dyestuff adsorbed onto serum protein of the paper is proportional to the content of the latter. This assumption, however, is not necessarily correct, since the amount of dye per unit of protein does not keep constant, if the concentration of adsorbed protein increases more than a definite one. This was shown by the present author's experiment. Accordingly, in order to get the true values, extinction readings must be corrected in regard to protein concentration by the calibration curve which was obtained here.
When the dyestuff of the paper was washed out by 0.5% acetic acid aqueous solution containing free chlorine in it, the adsorbed dyestuff of the protein as well as of the paper is simultaneously decolored to such an extent as to interfere with the extinction readings. Thus the percentage of each serum protein fraction will not indicate the true one. In order to prevent this effect by decoloration of the dyestuff, a drop of 5% sodium thiosulfate solution was added to 2 liters of acetic acid solution, the water of which was from city water supply. By this method the author obtained the satisfactory pattern of the serum protein fractions which indicated minimum standard errors.View full abstractDownload PDF (616K) -
Tetsuro Yoshida, Shigeo Takahashi1958 Volume 5 Issue 2 Pages 61-68
Published: November 15, 1958
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSA method for dispensing with the dialysis of serum and plasma samples prior to electrophoresis according to Tiselius' method in free solution has been described.
One volume of the sample was mixed with threee volumes of the phosphate buffer solution, and the mixture was analyzed by using a certain buffer solution (pH 7.9 and μ 0.15) as the supernatant in the electrophoretic run.
The electrolyte compositions of these buffer solutions were shown.
In cases where no pathological changes in the electrolyte composition of the blood cun be observed, a close agreement between electrophoretic analyses carried out accoring to this method and according to the conventional one with dialysis has been demonstrated on eight samples of the sera of healthy men and on eight samples of the sera and on four samples of the plasmas (obtained by means of heparinazed blood) of patients of various diseases.View full abstractDownload PDF (5746K) -
Yasuo Yamaguchi, Keiichi Nakakawaji, Hideo Sakabe, Shintaro Akimoto, A ...1958 Volume 5 Issue 2 Pages 69-71
Published: November 15, 1958
Released on J-STAGE: March 31, 2009
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K. Shimao1958 Volume 5 Issue 2 Pages 72-75
Published: November 15, 1958
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSDownload PDF (1935K) -
1958 Volume 5 Issue 2 Pages 77-81
Published: November 15, 1958
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSDownload PDF (828K)
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