Macrophages are a source of many important mediators of wound repair. Cells of an established macrophage-cell line (U-937) were exposed
in vitro to an 820 nm light source which was both coherent and polarized. the power densities used being either 400 mW/cm
2 or 800 mW/cm
2. The irradiation times were such that the energy densities to which the cells were exposed were 2.4 and 7.2J/cm
2 for both probes. Twelve hours after exposure the macrophage-conditioned medium was removed and placed on 3T3 fibroblast monolayers. Fibroblast proliferation was assessed over a four-day period. By four days after the addition of medium conditioned by macrophages exposed to an energy density of 2.4 J/cm
2, there was a statistically significant difference in fibroblast number between the 400 mW/cm
2- and 800 mW/cm
2 treatments, 800mW/cm
2 producing greater cell proliferation. However. there was no significant difference between the effects of sham irradiation and 400 mW/cm
2. In contrast, after the addition of medium conditioned by macrophage exposed to an energy density of 7.2 J/cm
2, 400 mW/cm
2 treatment produced a significantly greater increase in fibroblast number than sham irradiation. There was no significant difference in cell number between the sham irradiated and 800 mW/cm
2 irradiated samples, although there was a significant difference between the 400 mW/cm2 and the 800 mW/cm
2. 400 mW/cm
2 producing greater cell proliferation.
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