Effects of clonidine, an imidazoline derivative as well as α
2-adrenoceptor agonist, on carbachol (CCh)-evoked contraction in guinea pig ileal smooth muscle were studied using isometric tension recording. To investigate the cellular mechanisms of the inhibitory effect of clonidine, its effects on CCh-evoked nonselective cationic current (I
CCh), voltage-dependent Ca
2+ current (I
Ca) and voltage-dependent K
+ current (I
K) was also studied using patch-clamp recording techniques in single ileal cells. Clonidine inhibited the contraction evoked by CCh (1 μM) in a concentration-dependent manner with an IC
50 valve of 61.7 ± 2.5 μM. High K
+ (40 mM)-evoked contraction was only slightly inhibited even when clonidine was used at 300 μM. Externally applied clonidine inhibited I
CCh dose-dependently with an IC
50 of 42.0 ± 2.6 μM. When applied internally via patch pipettes, clonidine was without effect. An I
CCh-like current induced by GTPγS was also inhibited by bath application of clonidine. None of KU14R and BU224, both imidazoline receptor blockers, and yohimbine, an α
2-adrenergic blocker, significantly affects the inhibitory effect of clonidine on I
CCh. Clonidine (300 μM) only slightly decreased membrane currents flowing through voltage-gated Ca
2+ channels or K
+ channels. These data indicate that clonidine relaxes smooth muscle contraction produced by muscarinic receptor activation and suggest that the effect of clonidine seems due mainly to inhibition of I
CCh via acting directly on the involved cationic channel.
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