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Volume 30, Issue 2
Displaying 1-7 of 7 articles from this issue
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PART I. STUDIES ON MECHANISMS OF LEUKOCYTE ADHERENCE INHIBITIONTADATOSHI KURATSUJI1981 Volume 30 Issue 2 Pages 53-63
Published: 1981
Released on J-STAGE: March 27, 2009
JOURNAL FREE ACCESSMechanisms of leukocyte adherence inhibition (LAI) phenomenon were investigated by utilizing Halliday's hemacytometer method. When guinea pig were immunized with dinitrophenyl conjugated bovine serum albumin (DNP-BSA) with Freund's complete adjuvant, the adherence of their leukocytes to glass surface is markedly inhibited by the presence of BSA or PPD, but not by bovine gammaglobulin (BGG). Percent leukocyte adherence inhibition (% LAI Mean ± S. D.) is 46.6 ± 1.9, 42.7 ± 1.7 and 3.2 ± 8.2, respectively. When immunized with DNP-BGG, % LAI is 34.5 ± 5.8, 42.9 ± 2.6 and 6.9 ± 3.9 by incubated with BGG, PPD and BSA, respectively. Thus LAI phe-nomenon is carrier specific reaction. While the whole blood leukocytes react with the specific antigen and showed positive LAI, but not polymorphonuclear leukocytes. This result indicates that mononuclear leukocytes (MNL), that is lymphocytes and monocytes, are effector and indicator cells. Adherence of leukocytes from guinea pigs producing antibody are not inhibited by antigen. Sera containing antibody or antigen-antibody complex did not sensitize normal cells to antigen. Lymphokine can perform LAI phenomenon to normal leuko-cytes, but a half to twelve hours culture supernatant of MNL with the specific antigen can not. These findings indicate that LAI psenomenon is the expres-sion of cell-mediated immunity, and partly mediated by soluble substances other than lymphokine.View full abstractDownload PDF (586K) -
PART II. CLINICAL APPLICATION OF LAI TEST TO DETECT DELAYED TYPE HYPERSENSITIVITY IN INFANTS AND CHILDRENTADATOSHI KURATSUJI1981 Volume 30 Issue 2 Pages 65-69
Published: 1981
Released on J-STAGE: March 27, 2009
JOURNAL FREE ACCESSThe leukocyte adherence inhibition (LAI) test were performed by utilizing Halliday's hemacytometer method. Total 47 healthy individuals (4 tuberculin purified protein derivative-positive, 12 Candida-positive, 8 house dust-positive and 23 delayed skin test-negative), aged 3 weeks to 6 years old, were blood donors. Mononuclear leukocytes (MNL) were isolated from the peripheral blood, and used as effector and indicator cells. Mean percent leukocyte adherence inhibition (% LAI) of MNL from persons with positive delayed skin test is 34.78 ± 12.40. When skin test is negative, mean % LAI is 9.64 ± 8.20. There is a significant difference between them. (p<0.001) The LAI test seems to be usable to detect cell-mediated immunity, especially in infants and children, partly because only a small volume of blood is re-quired to the LAI test.View full abstractDownload PDF (256K) -
PART II. FECAL EXCRETION OF THE HEAVY METALSSOICHIRO IWAO, MINORU SUGITA, KENZABURO TSUCHIYA1981 Volume 30 Issue 2 Pages 71-82
Published: 1981
Released on J-STAGE: March 27, 2009
JOURNAL FREE ACCESSA total of 216 men, 50 to 70 years of age, who were clinically healthy, in particular without proteinuria or glucosuria, were the subjects of fecal heavy metal analysis, with approximately 30 to 40 men from three Cd-polluted and matched control areas in Japan. The amount of fecal heavy metals (Cd, Pb, Cu and Zn) did not differ greatly from those metal amounts in daily foods. Fecal Cd is thought as a good indicator of Cd exposure. The inhabitants of 'Cd-polluted areas excreted 2.5 times more Cd in feces than the control area inhabitants. The daily balance study for the heavy metals revealed negative balance which did not differ significantly by area. This loss of the daily heavy metal balance may be due to other sources, such as inhalation by lung, resulting in the increase of those in the feces.View full abstractDownload PDF (651K) -
TOSHIHIKO ARAI, SADAO KOMATSU1981 Volume 30 Issue 2 Pages 83-87
Published: 1981
Released on J-STAGE: March 27, 2009
JOURNAL FREE ACCESSIncidences of colicinogenic strains in the Escherichia coli strains isolated from various clinical materials were examined. The incidences were higher in the order in the strains isolated from pustular materials, feces, sputa and urine. Approximately 32% of the pustular strains were found to be colicino-genic. Heat stability of these colicins were examined and incidences of the heat stable colicins were found to be higher in the strains from pustular mate-rials than the strains from any other materials. Some of the colicins which retained their activity after treatment at 100°C for 30 minutes were con-centrated and tested for the cellophane membrane permeability and the re-sistance to methanol, acid and alkaline. They were all found to be permeable through cellophane membrane and resistant to all the chemicals.View full abstractDownload PDF (289K) -
PART III. URINARY EXCRETION OF THE HEAVY METALSSOICHIRO IWAO, MINORU SUGITA, KENZABURO TSUCHIYA1981 Volume 30 Issue 2 Pages 89-96
Published: 1981
Released on J-STAGE: March 27, 2009
JOURNAL FREE ACCESSThe four heavy metal concentrations (Cd, Pb, Cu and Zn) in the 216 urine samples from the residents of three Cd-polluted and matched control areas were determined. And the differences of these metal distributions as well as the estimation of possible mechanisms of the heavy metal excretion in the urine in relation to the dietary intake were statistically analyzed.
Urinary Cd in the polluted area residents was higher than that of the control area residents, however, the ratio of urinary excretion of Cd among the polluted area residents to the control area residents was smaller than the ratio of fecal Cd excretion in the two area residents. This may suggest the more accumulation and retention of Cd among the Cd-polluted area residents, resulting in the appearance of less Cd concentration in the urine.
The urinary Pb concentration in the polluted area residents also showed higher than those of the control area residents; this tendency was consistent with the results from the dietary and fecal Pb analyses. The essential metals in the urine, Cu and Zn, showed the different tendency that urinary Zn con-centration did not differ by area, whereas that urinary Cu did differ by area. These were not consistent with the results from the fecal analysis, suggesting the different metabolism of the essential metals in the human body.View full abstractDownload PDF (427K) -
MASAMICHI HAYAKAWA, SEIDO JITSUKAWA, HIDECHIKA KINOSHITA, HIROSHI TAZA ...1981 Volume 30 Issue 2 Pages 97-103
Published: 1981
Released on J-STAGE: March 27, 2009
JOURNAL FREE ACCESSOur retrograde brushing catheter technique to obtain cytology specimens from upper urinary tract is useful to increase the diagnostic accuracy. Con-ventional cytology in 9 patients in study group and in 40 control group both with transitional cell carcinoma showed 30 percent and 35 percent of positive rate, respectively, while the brushing technique in the 9 patients increase the positive rate up to 89 percent and the difference is statistically significant (P<0.5%). There were no false positive in benign cases.View full abstractDownload PDF (4466K) -
MAKOTO HATA, KAZUO MATSUSHITA, SEIDO JITSUKAWA, MASARU MURAI, HIDECHIK ...1981 Volume 30 Issue 2 Pages 105-113
Published: 1981
Released on J-STAGE: March 27, 2009
JOURNAL FREE ACCESSTwo patients, a 33-year-old female and 28-year-old male, were presented with sudden onset of total gross hematuria leading to non visualization of the involved kidney on excretory urogram. Diagnosis was made of multiple intra-renal arteriovenous malformation having cirsoid appearance in the selective renal arteriogram. The multiplicity and gross hematuria caused by eroding renal tissue were considered to be unquestioned indication for surgical inter-vention. Several modes of therapy aiming at the conservation of the kidney have been considered, such as partial nephrectomy, transcatheter emboliza-tion and vascular ligation. Vascular ligation can be accomplished with the minimal loss of renal tissue, but because of its technical difficulty, its indica-cation is strictly limited. The modern technology of ex vivo renal surgery, however, can expand the realm of vascular ligation even to the intrarenal arteriovenous fistula.
The patients were treated successfully by ligation and division of each feeding artery by ex vivo technique and autotransplantation. Selective arte-riography of the grafted kidney taken at the 18th month after operation de-monstrated no evidence of recurrence of A-V malformation.
Review of English literatures revealed that present two cases are the first publication of multiple intrarenal A-V malformation in which kidney conserving vascular surgery was carried out by utilizing ex vivo technique.View full abstractDownload PDF (3284K)
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