1) The seeds of Japanese timothy which had been treated with streptomycin solution (200γ/cc) at 5°C for 4 days were rinsed with pure water for 24 hours at 5°C prior to the cultivation on water medium, and it was found that nearly 90% of the leaves were affected by streptomycin and became white without forming chlorophyll.
2) When streptomycin-treated seeds were rinsed, prior to the cultivation, in water of pH 4.0, about 85% of the leaves recovered chlorophyll formation, none of them recovered in water of pH values more alkaline than 5.0.
3) Chlorophyll formation was also recovered in the majority of the leaves when streptomycin-treated seeds were rinsed, prior to the cultivation, for 24 hours at 5°C in 0.07N solutions of monovalent metal salts such as Na-, K-, and Li-salts. It was considered that metal ions should be playing an important role in this recovery but a certain kind of anion could have participated in this result.
4) Solutions of bivalent metal salts such as Ca-, Ba-, Mg-, Sr-, and Zn-salt presented almost the same results as those of monovalent ones at the concentration of 0.007N, namely the efficacy rate of bivalent metal salts to monovalent ones was approximately 10:1. The author is indebted to Dr. Hideo Moriyama, the Director of this institute, for his many valuable advices and encouragements.
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