巻号一覧
44 巻, 10 号
「ナノバイオのための超解像顕微鏡の新たな応用展開」特集号
選択された号の論文の10件中1～10を表示しています
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「ナノバイオのための超解像顕微鏡の新たな応用展開」特集号
特 集
レーザー解説
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居波 渉, 川田 善正2016 年 44 巻 10 号 p. 642-
発行日: 2016年
公開日: 2020/12/17
ジャーナル オープンアクセスPDF形式でダウンロード (180K) -
岡田 康志2016 年 44 巻 10 号 p. 643-
発行日: 2016年
公開日: 2020/12/17
ジャーナル オープンアクセスFluorescent live cell imaging has been widely used in biomedical studies since the introduction of the green fluorescent protein technology. However, many subcellular structures with sizes less than 200 nm are beyond the resolution limit of optical microscopes with visible light, therefore, super-resolution fluorescence microscopy attracts strong interest. In this review, the basic principles of several popular super-resolution fluorescence microscopy techniques are reviewed along with their biomedical applications especially live cell imaging.抄録全体を表示PDF形式でダウンロード (724K) -
渡辺 梢, 藤田 克昌2016 年 44 巻 10 号 p. 648-
発行日: 2016年
公開日: 2020/12/17
ジャーナル オープンアクセスRecent development in super resolution microscopy has enabled noninvasive observation of various kinds of specimens with high spatial-resolution. Despite the potential of the chemical analysis in Raman microscopy, high-resolution analytical imaging has been challenging due to the weak efficiency in Raman scattering. We introduce structured illumination to spontaneous Raman microscopy in order to realize a technique for large-area and high-spatial resolution micro-spectroscopic imaging. We developed a structured line illumination Raman microscope using two beam interference in a line illumination and demonstrated high-resolution Raman imaging of polymer nanoparticles and a biological tissue. The results indicate that the high resolution Raman imaging would strongly enhance the analytical capability in chemical imaging of medical, biological and industrial materials.抄録全体を表示PDF形式でダウンロード (607K) -
磯部 圭佑, 戸田 圭亮, 緑川 克美2016 年 44 巻 10 号 p. 653-
発行日: 2016年
公開日: 2020/12/17
ジャーナル オープンアクセスSuper-resolution optical microscopy that operates beyond the diffraction limit has become a powerful tool for investigating biological phenomena. However, it is difficult to achieve super-resolution imaging at deeper penetration depth in biological tissue. Since the in-focus signal intensity in super-resolution microscopy is very low, the in-focus signal is buried easily in the background light generated in out-offocus regions. Therefore, the background suppression techniques are essential for super-resolution deep imaging. Although nonlinear optical microscopy has the capability to suppress the out-of-focus background, the out-of-focus background generates in the deep imaging of scattering tissue and limits the observable imaging depth. We demonstrated not only the resolution enhancement but also the background suppression in nonlinear optical microscopy by spatio-temporal control of the excitation pulses.抄録全体を表示PDF形式でダウンロード (637K) -
大友 康平, 根本 知己2016 年 44 巻 10 号 p. 658-
発行日: 2016年
公開日: 2020/12/17
ジャーナル オープンアクセスLaser scanning microscopy by a multi-photon process is a powerful tool for the visualization of microstructures in living specimens because of its merits, locality of the excitation, less invasion and deep penetration. However, the spatial resolution of this methodology is inferior to single-photon excitationbased confocal laser scanning microscopy and recent super-resolution microscopies. On the other hand, stimulated emission depletion (STED) microscopy, one of the most common super-resolution microscopies, is based on a laser scanning microscopy system. Therefore, by combining these methodologies, recently attempts of the visualization of nano-structures in biological specimens have been successful. These methodologies potentially have both merits of deep penetration and high spatial resolution. In this article, we describe them in detail including our attempts to develop a new twophoton excitation STED microscopy and discuss future research and new technologies in this field.抄録全体を表示PDF形式でダウンロード (824K) -
岡野 真之, 岡本 亮, 竹内 繁樹2016 年 44 巻 10 号 p. 663-
発行日: 2016年
公開日: 2020/12/17
ジャーナル オープンアクセスQuantum technologies harness the intrinsic nature of quantum physics to beat limitations of classical methods. Recently, the application of quantum entanglement to metrology has attracted much attention. Quantum optical coherence tomography (QOCT) using two-photon interference between entangled photon pairs can overcome certain problems with classical optical coherence tomography (OCT). As the resolution of OCT becomes higher, degradation of the resolution due to dispersion becomes more critical. Here we show our work on the realization of 0.54 μm resolution two-photon interference, which surpasses the current record resolution 0.75 μm for OCT. In addition, the resolution for QOCT showed almost no change even when a 1-mm-thick water was inserted in the path of the interferometer. The results presented here represent a breakthrough for the realization of ultrahigh-resolution quantum metrology. Our work will open up possibilities for medical and biological applications.抄録全体を表示PDF形式でダウンロード (489K)
レーザーオリジナル
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益田 有里子, 居波 渉, 川田 善正2016 年 44 巻 10 号 p. 668-
発行日: 2016年
公開日: 2020/12/17
ジャーナル オープンアクセスWe investigated fluorescent nano diamonds as fluorescent markers for a direct electron-beam excitation (D-EXA) optical microscope. The D-EXA microscope excites the nanometric region of the specimen in an atmospheric pressure by electron beam irradiation. We used the fluorescent nano diamonds as fluorescent makers because of their less photobleaching, low cytotoxity, high efficiency of emission. They also can be excited both photoluminescence and cathodoluminescence, so it is possible to demonstrate the correlative observations between the D-EXA microscope and a fluorescent confocal laser microscope. We measured the cathodoluminescent spectra of fluorescent nano diamonds, and presented that the D-EXA microscope can overcome the diffraction limit.抄録全体を表示PDF形式でダウンロード (731K)
再掲載
レーザー解説
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中井 光男, 有川 安信, 西村 博明, Hui CHEN2016 年 44 巻 10 号 p. 672-
発行日: 2016年
公開日: 2020/12/17
ジャーナル オープンアクセスRelativistic electron-positron plasmas are believed to have an important role in the energetic phenomena in the universe, in the form of collimated jets or relativistic flow from pulsars and black-holes. Development of ultra-intense lasers are making possible to create this unique entity on the earth and open up an experimental frontier of astrophysics. Here presented are the review on the relativistic electron-positron plasma generation via Bethe-Heitler process by using high power lasers. It was demonstrated that more than 1012/kJ of pair creation per laser energy was possible and formation of the relativistic pair plasmas, which make it possible to investigate the collisionless shock in the laboratory, is expected by up-coming large laser facility with 10-kJ output energy. Relevant experiment at Osaka University is also reported.抄録全体を表示PDF形式でダウンロード (1081K)
一般論文
レーザーオリジナル
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吉松 剛, 五島 敬史郎, 青木 道宏, 津田 紀生, 山田 諄2016 年 44 巻 10 号 p. 679-
発行日: 2016年
公開日: 2020/12/17
ジャーナル オープンアクセスWhen part of the scattering light from a target is incident to the laser cavity, the terminal voltage of a semiconductor laser diode slightly fluctuates. This voltage fluctuation has information related to the distance to the target. However, terminal voltage fluctuation also has much noise. Since the discrimination of distance signals with much noise is very difficult, we propose a statistical signal processing that utilizes all of the information in terminal voltage fluctuation. We constructed a system using a Field-Programmable Gate Array (FPGA) that can measure a distance of 21 to 45 cm with an average error of about 2%.抄録全体を表示PDF形式でダウンロード (662K)
レーザー技術ノート
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岩井 克全, 高久 裕之, 宮城 光信, 石 芸尉2016 年 44 巻 10 号 p. 684-
発行日: 2016年
公開日: 2020/12/17
ジャーナル オープンアクセスFor medical application by using the infrared laser, it is necessary to keeps high mechanical strength of the hollow fibers. We chose a stainless tube as the base material for the hollow fibers. In order to reduce roughness of inner surface of stainless tube which causes the additional transmission loss. We proposed a new material to fabricate a low-loss-silver-hollow fiber based on a stainless tube. The new material is an acrylic-silicon resin material which is used as a buffer polymer to the inner walls of stainless tubes for a low-loss characteristic. We also discuss the transmission properties of visible pilot beam and Er: YAG laser light in the silver-hollow fibers. The loss for the 550-μm-bore size, 1-m-length silver-hollow fiber was 5.9dB under straight configuration, and 8.8dB under the configuration of a 180 degree bending with a 20.25-mm bending radius at the wavelength of 650 nm.抄録全体を表示PDF形式でダウンロード (943K)
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