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Simulation using Rolling Jig
Haruna Sakurai, Jun Shimizu, Takeyuki Yamamoto, Libo Zhou, Teppei Onuk ...
Pages
97-98
Published: March 01, 2020
Released on J-STAGE: September 03, 2020
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Investigation on processing principle with high speed camera
Makoto Yokei, Masaki Michihata, Kiyoshi Takamasu, Satoru Takahashi
Pages
99-100
Published: March 01, 2020
Released on J-STAGE: September 03, 2020
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Processing technology for manufacturing fine devices and next-generation functional parts are demanded. In this study, by focusing on the optical trapping potential due to the electric field intensity gradient, we have proposed 3-dimensional processing method by using nanoparticles without being limited by the diffraction limit. In this paper, we have investigated processing principle of 3-D pillar structure by introducing a high speed camera and observing in-process accumulating and localizing droplet carriers by optical trapping potential.
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Shunnosuke Kodera, Tomoki Watanabe, Yoshiyuki Yokoyama, Takeshi Hayaka ...
Pages
101-102
Published: March 01, 2020
Released on J-STAGE: September 03, 2020
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In recent years, biomimetic robots having soft structures like living organisms have been actively studied. They can move flexibly such as living organisms. The common feature of these robots and many organisms is having not only soft structures but also rigid structures. Therefore, we focused on that soft-rigid hybrid structure has important role in biomimetic robots. However, there are few biomimetic robots having soft-rigid hybrid structure in microscale. Soft-rigid hybrid structure is also important in microscale. For instance, Euglena has cytoskeleton and cell membrane as rigid structures, and flagellum as soft structures. Thus, we aim to fabricate biomimetic microrobot having soft-rigid hybrid structure. In this study, we propose a microrobot having soft-rigid hybrid structure. As hard structures, SU-8 which is hard photoresist is used. As soft structures, bioresist which is a temperature-responsive gel is used. Bioresist swells by absorbing water at lower temperature than 32 °C and shrinks by releasing water at higher than the temperature. The proposed robot is fabricated by using multilayered photolithography processes. We mixed bioresist with graphene as a light absorber to actuate this soft structures by near infrared laser irradiation. We succeeded in fabrication and actuation of the proposed robot by light irradiation.
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Naoyuki Otsuka, Takeharu Hukuhara, Hiroshi Kobayashi, Toshiyuki Horiuc ...
Pages
103-104
Published: March 01, 2020
Released on J-STAGE: September 03, 2020
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Wataru Sakakibara, Yukihiro Domon, Hiroshi Kobayashi, Toshiyuki Horiuc ...
Pages
105-106
Published: March 01, 2020
Released on J-STAGE: September 03, 2020
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Copper Electrodeposition into Micro Trenches
Yuki Otsuka, Takanori Akita, Masaru Irita, Masanori Hayase
Pages
107
Published: March 01, 2020
Released on J-STAGE: September 03, 2020
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Mask Formation by Talbot Lithography
Yohei Kato, Arata Kaneko, Ryu Ezaki, Yasuhiro Mizutani, Yasuhiro Takay ...
Pages
108-109
Published: March 01, 2020
Released on J-STAGE: September 03, 2020
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Keisuke Kanada, Arata Kaneko
Pages
110-111
Published: March 01, 2020
Released on J-STAGE: September 03, 2020
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Fuga Yoshida, Kazumori Yoshida, Yuta Suzuki, Jun-ya Iwasaki, Hiroshi K ...
Pages
112-113
Published: March 01, 2020
Released on J-STAGE: September 03, 2020
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Shutaro Nakamura, Nobuyuki Moronuki
Pages
114-115
Published: March 01, 2020
Released on J-STAGE: September 03, 2020
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Taishi Yaguchi, Nobuyuki Moronuki
Pages
116-117
Published: March 01, 2020
Released on J-STAGE: September 03, 2020
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Mizuki Yamamoto, Kosuke Inada, Takashi Nisisako
Pages
118-119
Published: March 01, 2020
Released on J-STAGE: September 03, 2020
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We report PDMS-stainless steel microfluidic devices for generating droplets. Microfluidic channels are made in PDMS slab via soft lithography, while the stainless-steel parts were made by mechanical machining and wire-electric discharge machining, and both parts are bonded together by excimer laser. By infusing pure water and oleic acid into the device, we could produce water-in-oil droplets.
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Pore Size Enlargement of Porous Catalyst Layer
Soshiro Kobune, Keita Takahashi, Masaru Irita, Masanori Hayase
Pages
120
Published: March 01, 2020
Released on J-STAGE: September 03, 2020
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Daiki Itou, Hitoshi Suwabe, Ken-ichi Ishikawa
Pages
121-122
Published: March 01, 2020
Released on J-STAGE: September 03, 2020
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Yuki Konishi, Hitoshi Suwabe, Mayuko Takahashi, Ken-ichi Ishikawa
Pages
123-124
Published: March 01, 2020
Released on J-STAGE: September 03, 2020
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Jiayu Liu, Satoshi Sakamoto, Mitsugu Yamaguchi, Yasuo Kondo, Kenji Yam ...
Pages
125-126
Published: March 01, 2020
Released on J-STAGE: September 03, 2020
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Keitaro Fujii, Masayuki Takahashi, Toshihiko Wada
Pages
127-128
Published: March 01, 2020
Released on J-STAGE: September 03, 2020
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Soshi Iwatsuki, Hirofumi Hidai, Souta Matsusaka, Akira Chiba, Noboru M ...
Pages
129-130
Published: March 01, 2020
Released on J-STAGE: September 03, 2020
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Yanhua Zou, Shota Ichikawa
Pages
131-132
Published: March 01, 2020
Released on J-STAGE: September 03, 2020
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Youhei Okubo, Ryunosuke Sato, Yanhua Zou, Taiki Koma, Toshiki Minagawa
Pages
133-134
Published: March 01, 2020
Released on J-STAGE: September 03, 2020
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Investigation of machining mechanism
Yanhua Zou, Ryunosuke Satou, Ozora Yamazaki
Pages
135
Published: March 01, 2020
Released on J-STAGE: September 03, 2020
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Hideki Kawakubo, Unkai Sato, Shuichi Murata
Pages
136-137
Published: March 01, 2020
Released on J-STAGE: September 03, 2020
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Shogo Chiba, Takayuki Kusumi, Yoichi Akagami, Takayuki Tokoroyama
Pages
138-139
Published: March 01, 2020
Released on J-STAGE: September 03, 2020
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Narumasa Miyazaki, Yusuke Ootani, Nobuki Ozawa, Momoji Kubo
Pages
140
Published: March 01, 2020
Released on J-STAGE: September 03, 2020
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Planarization of GaN substrate
Akihisa Kubota
Pages
141
Published: March 01, 2020
Released on J-STAGE: September 03, 2020
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Effect of introducing micro bubbles
Akihisa Kubota
Pages
142
Published: March 01, 2020
Released on J-STAGE: September 03, 2020
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Ryo Fukuzaki, Seiichi Suda, Masashi Hase
Pages
143
Published: March 01, 2020
Released on J-STAGE: September 03, 2020
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Hiroki Hoshiya, Tomohisa Tanaka, Satoshi Inagaki
Pages
144-145
Published: March 01, 2020
Released on J-STAGE: September 03, 2020
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Mohan Loganathan, Nandhini Balasubramaniam, Srabani Kar, Keerthiga Gan ...
Pages
146-147
Published: March 01, 2020
Released on J-STAGE: September 03, 2020
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Treating critical diseases in recent years demands significant research on intracellular delivery in the field of cell biology and medical science. The ability to deliver external molecules into living cells with high cell viability and transfection efficiency is of vast interest in cell biology for applications in diagnostics, drug delivery, and therapeutic development. Optoporation is emerging and has become popular for intracellular delivery in the last few years, because of less invasiveness. Still, their delivery efficiency and viability need to be improved. We demonstrate Titanium-di-oxide (TiO2) nanostructure-based optoporation platform for versatile and highly efficient intracellular delivery, which is potentially applicable for cellular therapy and diagnostics. TiO2 nanostructures were formed on titanium sheets using the electrochemical anodization technique. After the fabrication of nanostructures, cells (HCT-116, HeLa) were cultured on the substrate overnight. The confluent cells on nanostructures were then introduced with cargo material and an optoporation experiments were conducted. The experimental parameters such as cargo concentration, wavelength of a nanosecond pulsed laser, pulse duration, etc. are varied to optimize the results to achieve high cargo delivery and cell viability.
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Evaluation of Probability of Cell Membrane Perforation and Cell Viability
Miho Ishii-Teshima, Siti Syuhada Binti Azhar, Hirofumi Kurita, Rika Nu ...
Pages
148-149
Published: March 01, 2020
Released on J-STAGE: September 03, 2020
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Dinesh Krishna Thilagarani, Ganesh Kumar Mani, Kazuyoshi Tsuchiya, Kun ...
Pages
150-151
Published: March 01, 2020
Released on J-STAGE: September 03, 2020
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Maintaining a habitable zone is indispensable during the development of embryos inside eggs. The temperature inside the incubator plays a vital role in artificial incubation methods. The incubators should be able to adapt themselves to the embryo temperature. Present technologies measure the embryo temperatures by examining the outer shell of the eggs using contactless infrared thermometers. Infrared thermometers are highly susceptible to Relative-Humidity (RH) interference, leading to false positives. The present work dealt with an in-contact thin-film temperature sensor used to measure the temperature of the eggs and combine it with a closed-loop control system to regulate the temperature inside the incubator throughout the incubation period without human intervention. RH interference could be easily eliminated by in-contact sensors inside the incubator. The temperature control system is robust in terms of both physical fabrication and data transfer. It uses wireless protocols to transfer the temperature data, reducing the overall size of the system and offers maximum portability. The work is also expected to be combined with a web application for real-time monitoring of temperature and online control.
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Atsushi Fujii, Kento Hisa, Daisuke Yamane, Azusa Kage, Takayuki Shibat ...
Pages
152-153
Published: March 01, 2020
Released on J-STAGE: September 03, 2020
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Kenta Kato, Kodai Kawaguchi, Arata Kaneko
Pages
154-155
Published: March 01, 2020
Released on J-STAGE: September 03, 2020
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Yuha Koike, Hiroki Wada, Yoshiyuki Yokoyama, Takeshi Hayakawa
Pages
156-157
Published: 2020
Released on J-STAGE: September 03, 2020
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Mitigation of channel clogging (4)
Yuki Oka, Tatsuya Yoshizawa, Masaru Irita, Toshihiro Suzuki, Ryushin M ...
Pages
158
Published: March 01, 2020
Released on J-STAGE: September 03, 2020
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Guangchong Ji, Naotomo Tottori, Takashi Nisisako
Pages
159-160
Published: March 01, 2020
Released on J-STAGE: September 03, 2020
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In this study, we demonstrated the separation of lactic acid bacteria (LAB) in higher concentration yogurt sample (10 vol%) through a deterministic lateral displacement (DLD) micropillar arrays (pillars diameter:11.5 μm; the gap between the pillars: 3.5 μm) after vortexing. Before infusing the yogurt sample into the DLD arrays, the casein micelle aggregates were removed from the yogurt sample using vortexing. Then the prepared yogurt sample was infused into the DLD arrays with sheath fluid stream of pure water. The fluorescently stained LAB separated from yogurt sample were counted to evaluate the collection efficiency.
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Keisuke Mitomi, Kentaro Tanagi, Tiwari Anuj, Azusa Kage, Takayuki Shib ...
Pages
161-162
Published: March 01, 2020
Released on J-STAGE: September 03, 2020
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Naoki Yanagisawa, Kazuhiro Fujisaki, Kazuhiko Sasagawa, Takeshi Moriwa ...
Pages
163-164
Published: March 01, 2020
Released on J-STAGE: September 03, 2020
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Toshiyuki Matsui, Hiroaki Suzuki, Takeshi Hayakawa
Pages
165-166
Published: March 01, 2020
Released on J-STAGE: September 03, 2020
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Fluid mixing on microfluidic chip is important technology, on lab on a chip field. Conventional on-chip micromixer has succeeded in mixing by using external pumps. However, undesirable dead volume exists in tube connection part between external pump and microfluidic chip. Thus, conventional micromixers are not suitable for rare reagents or samples such as fluorescent reagents, protein and so on. Therefore, we have proposed on-chip mixing method based on a vibration-induced flow without pump. The proposed method utilizes vibration-induced flow which is the flow induced around vibrating microobject. In this study, we fabricated two designs of micropillar array for mixing. We evaluated mixing performance of these two design.
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Yoshinobu Okubo, Ryuta Nakamura, Takayuki Kusumi, Yoichi Akagami
Pages
167
Published: March 01, 2020
Released on J-STAGE: September 03, 2020
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Simultaneous Detection of Multiple Plant Viruses
Daigo Natsuhara, Keisuke Takishita, Kisuke Tanaka, Azusa Kage, Moeto N ...
Pages
168-169
Published: March 01, 2020
Released on J-STAGE: September 03, 2020
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Masashi Ikeuchi
Pages
170
Published: March 01, 2020
Released on J-STAGE: September 03, 2020
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Nobuhiro Kato, Namie Chihara, Mao Yokoyama, Atsushi Tanaka, Takashi Ak ...
Pages
171
Published: March 01, 2020
Released on J-STAGE: September 03, 2020
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Kentaro Kajiwara, Wataru Yashiro
Pages
172-173
Published: March 01, 2020
Released on J-STAGE: September 03, 2020
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Shingo Terashima, Chikako Tatsukawa, Tomokazu Takahashi, Masato Suzuki ...
Pages
174-175
Published: March 01, 2020
Released on J-STAGE: September 03, 2020
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A poly-lactic acid (PLA) microneedle array of maximum aspect ratio 18 was fabricated by Drawing lithography. In the fabrication method proposed in this study, melted PLA is stretched twice. Various aspect ratios can be set according to the length of the first stretching. The final aspect ratio and shape of microneedle can be controlled by the second stretching. Finally, puncture ability was evaluated using artificial skin and porcine skin.
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Takuto Kitakaze, Tomokazu Takahashi, Masato Suzuki, Seiji Aoyagi, Yosh ...
Pages
176-177
Published: March 01, 2020
Released on J-STAGE: September 03, 2020
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1st Report Microneedles combining LED therapy
Xiaobin Wu, Junichiro Kono, Nobuyuki Takama, Beomjoon Kim
Pages
178-179
Published: March 01, 2020
Released on J-STAGE: September 03, 2020
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Shunki Yamamoto, Tomokazu Takahashi, Masato Suzuki, Seiji Aoyagi, Tosh ...
Pages
180-181
Published: March 01, 2020
Released on J-STAGE: September 03, 2020
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Ganesh Kumar Mani, Kazuyoshi Tsuchiya
Pages
182-183
Published: March 01, 2020
Released on J-STAGE: September 03, 2020
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The detection of temperature and pH at the single-cell level is hoping for the great level of clinical importance for the early detection of many diseases like cancer, diabetes, etc. In this research work, we have fabricated a micro region pH and temperature sensors by series of processes like electrolytic polishing to create needle structure, deposition of electrode materials using sputtering for measurements and finally testing in various biological medium. The structural and morphological properties were analysed using XRD and FE-SEM. The fabricated tip of the microneedle probe is nearly 5µm analysed by laser microscope. pH testing was evaluated using fish egg and the found that results were significant. Seebeck co-efficient observed for the micro region temperature sensors is relatively low compared to theoretical values. Since the sensor works at micro region, the potential difference is easily disturbed by atmospheric anomalies. Hence, the accuracy of the sensor will be further improved in the future.
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Yuki Okumura, Tomokazu Takahasi, Masato Suzuki, Seiji Aoyagi, Tomoyuki ...
Pages
184-185
Published: March 01, 2020
Released on J-STAGE: September 03, 2020
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