The purpose of this study was to investigate the effects of ultra-sound irradiation and static stretching on immobilized Soleus muscle collagen of rats. After left hind limb of seventeen rats were immobilized for three weeks, the animals were randomly assigned to a ultrasound group (n=5), static stretching group (n=6), control group (n=6).
Hydroxyproline was determined for the estimation of the collagen content to represent as a percent of wet weight, the collagen content in neutral salt soluble, acid soluble and insoluble collagen.
There was no significant difference collagen content to represent as a percent of wet weight and collagen solubility between the static stretching group and control group.
In the case of ultrasound group, collagen content to represent as a percent of wet weight was increased significantly as compared with control group. The percentage of the salt soluble collagen to the total collagen did not change significantly as compared with control group. The percentage of the acid soluble collagen to the total collagen was increased significantly as compared with control group. The percentage of the insoluble collagen to the total collagen decreased significantly as compared with control group.
These results suggest that
1) Static stretching has no effect on solubility of immobilized Soleus muscle collagen, affected by intra and intermolecular cross-links.
2) Ultrasound irradiation has effect on solubility of immobilized Soleus muscle collagen, affected by intra and intermolecular cross-links.
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