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Volume 9 (1968)
- Issue 6 Pages 695-
- Issue 5 Pages 563-
- Issue 4 Pages 489-
- Issue 3 Pages 391-
- Issue 2 Pages 223-
- Issue 1 Pages 1-
Volume 9, Issue 4
Displaying 1-9 of 9 articles from this issue
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I. Effect of Platelet on FibrinolysisHiroshi SUGITA, Hideya SAKURAI, Mitsuto HASEGAWA1968 Volume 9 Issue 4 Pages 489-498
Published: 1968
Released on J-STAGE: October 20, 2008
JOURNAL RESTRICTED ACCESSThe influence of the blood platelet on fibrinolysis was studied by fibrin plate method, fibrin clot lysis test and thrombelastography. The results were as follows.
1. Platelets had antifibrinolytic action. The action was not spoiled by freezing and thawing, but it was destroyed by heating at 85°C for 30 minutes.
This inhibitory action was immediate and was accentuated by preparing the platelets with contact to fibrinolytic factors prior to its use. The action did not interfere the antifibrinolytic action of EACA but rather worked with it cooperatively.
2. In some of the cases with hematological disorders such as aplastic anemia, acute myelogenous leukemia or multiple myeloma, the antifibrinolytic action of platelets was weaker than that of normal individuals.
3. On the other hand fibrinolytic action of platelets (proactivator-like action) was also observed, which was activated by streptokinase.
This action was not affected by freezing and thawing, but destroyed by heating at 85°C for 30 minutes. The SK-activated fibrinolytic action of platelets was inhibited by EACA.
4. As the result of experiments in vitro, antifibrinolytic activity of normal human serum was 150-500 times higher than that of normal human platelets. The same result obtained from the experiments with normal rabbit serum and normal rabbit platelets.
5. In some of cases with hematological disorders such as acute leukemia or multiple myeloma, the antifibrinolytic activity both in the serum and platelets was decreased.
6. Plasma fibrin clot lysis time altered depending on the number or the quality of platelets in the plasma.View full abstractDownload PDF (764K) -
II. Effect of Fibrinolysis on Blood PlateletHiroshi SUGITA, Tetsuhei OGAWA, Hideya SAKURAI, Mitsuto HASEGAWA1968 Volume 9 Issue 4 Pages 499-503
Published: 1968
Released on J-STAGE: October 20, 2008
JOURNAL RESTRICTED ACCESSEffect of fibrinolysis on blood platelet was studied and following results were obtained.
1. Four groups of animals, such as normal dogs, thrombocytopenic dogs, normal rabbits and thrombocytopenic rabbits were kept in fibrinolytic state for one or two hours by intravenous injection of streptokinase. No remarkable changes in platelet counts were observed in any groups of animals.
2. Human platelet was incubated with plasmin and urokinase respectively for ten minutes. Fibrin clots were prepared mixing with normal untreated platelets, plasmin- or urokinase- treated platelets, and their lysis time were compared. Antifibrinolytic activity of platelets was suppressed by previous treatment with plasmin.
3. Lysing process of thin plasma clots prepared from platelet rich plasma of normal persons was observed with phasecontrast microscope and electron microscope. Platelets were deformed or destroyed under the hyperplasmic condition in which fibrin fibers were completely dissolved.View full abstractDownload PDF (832K) -
Its Influence on Plasma Euglobulin Lysis Time EstimationTakeo OSUGA, Kunio WAKABAYASHI, Michio MATSUDA, Mutsuyoshi KAZAMA, Tak ...1968 Volume 9 Issue 4 Pages 504-513
Published: 1968
Released on J-STAGE: October 20, 2008
JOURNAL RESTRICTED ACCESSThe influence of blood platelet on fibrinolysis was studied mainly by euglobulin lysis technique and the following results were obtained:
1. The euglobulin lysis time (eug LT) of plasma samples was prolonged in storage at higher temperature than 0°C, but almost no change observed at -20°C.
2. Platelet was proved to have inhibitory influence on the fibrinolytic activity of plasmas or their euglobulin fractions, which seemed to have some direct relationship with the concentration of platelet.
3. Platelet adsorbed proactivator for plasminogen in plasma and released it during washing, and the washed platelet adsorbed again the proactivator in plasma at mixing with it.
4. Platelet material obtained through its destruction, also exhibited inhibitory influence on fibrinolysis.
5. The activated contact (XII. and XI.) factors for coagulation in plasma were affected by platelet easier than their inactive forms and this might be also one of the causes for depressed fibrinolytic activity in platelet rich plasma samples.View full abstractDownload PDF (786K) -
I. Effects of Infusion of a Human Antihemophilic Globulin Preparation on Coagulation Defects of Hemophilia AKunio YOSHIDA, Keiichiro YOSHIOKA, Hiromu FUKUI, Noriharu TAGAWA, Mits ...1968 Volume 9 Issue 4 Pages 514-528
Published: 1968
Released on J-STAGE: October 20, 2008
JOURNAL RESTRICTED ACCESSStudies were made on the effects of administration of human antihemophilic globulin preparation (HAHG) upon coagulation defects of patients with hemophilia A. HAHG was a Cohn fraction I, obtained from a smallest pool of fresh normal plasma by the method of Nitschmann, Kistler and Joss, by the courtesy of Dr. Naito, MIDORI-JUJI Co. One dose of HAHG was prepared from 200 ml. of fresh normal ACD-plasma, for clinical use one dose of HAHG was dissolved into 100 ml. of Aq. dest.
Assay of 150 HAHG samples demonstrated 158±23.6%, about twice as much factor VIII as in normal plasma and also about twice as much fibrinogen (680±135 mg/dl). Negligible amounts were found in prothrombin, factor V, factor VII-complex, factor IX and factor X.
At various intervals after a single infusion of 100 ml. of HAHG to a patient with hemophilia A, determinations were made on factor VIII, fibrinogen, clotting time and serum prothrombin in paient blood. The infusion invited normalization of clotting time and prothrombin consumption as well increase of factor VIII for eight hours, while a slight increase (about 100 mg/dl.) of fibrinogen lasted for four hours.
The half life of factor VIII was estimated to be five hours, when a single dose of HAHG or 100 ml. of fresh normal plasma was given to patients with hemophilia A. Following a single dose infusion of HAHG, there developed a two-phase logarithmic diappearanse curve which consisted of an initial rapid phase and a second slower phase.
The factor VIII level reached a peak at 30 minutes after infusion of HAHG to patients with hemophilia A. This rise in factor VIII consentration ranged from 4 to 40 per cent when various doses of HAHG or fresh normal plasma ranging 2u/kg to 20u/kg were infused, which was almost equivalent to the expect one, and it was calculated that this observed rise after infusion of 1u/kg was about 2 per cent.View full abstractDownload PDF (1116K)
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Seiji ASANO, Yoshiro TSUJI, Hajime IMAMURA, Shiro MIWA, Harumi OHYAMA1968 Volume 9 Issue 4 Pages 529-533
Published: 1968
Released on J-STAGE: October 20, 2008
JOURNAL RESTRICTED ACCESSSix-years-old Japanese girl (N.O.) with congenital nonspherocytic hemolytic anemia was presented. Her parents are first cousins. Although erythrocyte pyruvate kinase (PK) showed above normal activity under usual assay conditions in this case, analysis of red cell glycolytic intermediates pointed a disturbance in glycolysis at the PK step, showing Oigh phosphoenolpyruvate, 2-phosphoglycerate, 3-phosphoglycerate as well as 2, 3-diphosphoglycerate concentrations and low ATP level. Further studies on partially purified erythrocyte PK from the patient revealed marked elevation of Michaelis constant for phosphoenolpyruvate and change in optimum pH as compared to that of normal subject.
It is most likely that there are at least several variants of PK as in case of glucose 6-phosphate dehydrogenase, and that the present case come under one of unusual PK variants. In this particular case, hemolytic anemia is probably caused by a production of an abnormal enzyme protein rather than decreased synthesis of normal PK.View full abstractDownload PDF (390K) -
Munemoto ITO, Yoshio YONAHARA1968 Volume 9 Issue 4 Pages 534-538
Published: 1968
Released on J-STAGE: October 20, 2008
JOURNAL RESTRICTED ACCESSThis is a 40-year-old female patient, who had suffered from typical atacks of acute leukemia 4½ years ago, and now she appears very well by usual treatment, such as blood transfusion, adrenocortical hormone and 6-mercaptopurine administration.
She was admitted to the 2nd Tokyo National Hospital in Feb., 1964 with cardinal complaints of palpitation and precordial discomfort, and stayed until Sept., 1966. On her admission, peripheral white blood cell count was 5,000 cm/m with 39.5% of leukemic cells, but the bone marrow specimen showed 98.2% of leukemic cells. In blood smear these leukemic cells had the Rieder-form nuclei with 2 to 3 nucleoles and oxydase reaction was almost negative. She had been suffering from several complications, such as serum hepatitis, genital hemorrhage, bronchitis, cystitis and pyelitis, for which she received conservative theapy with great success.
After discharge from the hospital, she has been completely well without any complaints and hematological examinations showed no abnormality up to present.
Such a long-term survival case of acute leukemia has not been reperted yet in Japan, so far.View full abstractDownload PDF (440K) -
Seiju ONODERA, Akira SHIBATA, Akira MIURA, Atsuo SUZUKI, Shinobu SAKAM ...1968 Volume 9 Issue 4 Pages 539-544
Published: 1968
Released on J-STAGE: October 20, 2008
JOURNAL RESTRICTED ACCESSImmunoelectrophoretic studies on four cases with IgD-myeloma were performed using various antisera. All these four cases did not form the so-called M-bow in the immunoelectrophoresis using the anti-IgG, anti-IgA and anti-IgM serum, but produced the distinct M-bow with the antiserum against the type L, Bence Jones protein. The anti-IgD serum and the anti-Bence Jones protein sera (type K and type L) should be employed when an M-bow is not formed with the anti-whole human serum in the immunoelectrophoretic pattern of a multiple myeloma serum.
To determine the antigenic type of a myeloma protein may lead to the discovery of a new type of immunoglobulins and can be a valuable tool in persuing the role of immunoglobulins in the biological system.View full abstractDownload PDF (723K) -
Motoharu UESU, Tosihisa KATAOKA, Hazime ANDO, Masahiko ONO, Tsunemituu ...1968 Volume 9 Issue 4 Pages 545-551
Published: 1968
Released on J-STAGE: October 20, 2008
JOURNAL RESTRICTED ACCESSThe common type of G6PD deficiency hemolytic anemia usually manifests their symptome immediatry after taking a certain drugs or fava bean.
The present case is 9 year old Japanese boy hemizygote with G6PD deficiency congenital nonspherocytic hemolytic anemia that is a rare type of manifestation of G6PD deficiency.
Each of hemolytic episodes is apparently induced by an acute infection. The activity of G6PD of the red cell of propositus is O unit/100ml erythrocytes in contrast with normal value of 188±25 units/100ml erythrocytes measured by a modified Korberg-Horecker's method, and percentage of ghost cell accounts for 86% comparing with normal value of 0.1±0.1% by the methemoglobin reduction test. Starch gel elctrophoresis of hemolysates of the patient suggested that his G6PD was type B.
Three female heterozygotes were detected from family members by methemoglobin reduction test with intermediately high frequency of ghost cells but no clinical or hematological abnormalities in those family members were noted.
Histochemical methemoglobin reduction test was found to be an useful and reliable procedure for the detection of the carrier state.View full abstractDownload PDF (632K) -
Hiroko NINOMIYA, Eiken OZAWA, Hatsunori OTA, Hiroshi OKUMURA, Koyo TAK ...1968 Volume 9 Issue 4 Pages 552-559
Published: 1968
Released on J-STAGE: October 20, 2008
JOURNAL RESTRICTED ACCESSA 34-year-old male with cholecystopathy and cryoglobulinemia was reported. Immunological examination revealed that the cryoglobulin of this patient is the “mixed type” consisting of IgG & IgM.
Rabbits were immunized with the purified cryoglobulin. The antiserum obtained reacted chiefly with IgG & IgM. Antigenic differences among the components of the cryoglobulin from this patient and of other three cryoglobulins of mixed type were not demonstrated using the antiserum.
Intracutaneous injection of the cryoglobulins failed to transfer the cold-hypersensitivity to normal recipients. Sera of four patients with cryoglobulinemia studied in our clinic were shown to have decreased or almost no complement-activity. But evidences were obtained that complements do not participate in cryoprecipitation. Effect by changing of the salt concentration on cryoprecipitation was also investigated.View full abstractDownload PDF (1273K)
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