Agricultural and Biological Chemistry
Online ISSN : 1881-1280
Print ISSN : 0002-1369
ISSN-L : 0002-1369
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Volume 25, Issue 7
Displaying 1-14 of 14 articles from this issue
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  • Studies on the Mechanism of Mold Protease Production
    Part III. Activating Factors of Inactive Protease in Cell-Free Extracts of Aspergillus sojae KS
    Kishiro YAMAMOTO
    1961 Volume 25 Issue 7 Pages 519-526
    Published: 1961
    Released on J-STAGE: February 07, 2011
    DOIhttps://doi.org/10.1271/bbb1961.25.519
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    The activating factors of the inactive protease in cell-free extracts obtained from growing mycelia of Aspergillus sojae KS were studied. It was found that the several kinds of metals were involved in activation, and the role of these metals on the activation was investigated. The velocity of the activation was maximal around pH 10 as well as around pH 5. It was proved that a kinase (enzyme) capable of activating the inactive protease in alkaline solution does exist in the cell-free extract.
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  • Studies on the Carbohydrates of Soybeans
    Part VI. Component Sugars of Fractionated Polysaccharides, Especially Identification of Fucose in Some Hemicelluloses
    Sin'itirô KAWAMURA, Teiiti NARASAKI
    1961 Volume 25 Issue 7 Pages 527-531
    Published: 1961
    Released on J-STAGE: February 07, 2011
    DOIhttps://doi.org/10.1271/bbb1961.25.527
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    Soybean polysaccharides were fractionated by using water, ammonium oxalate, and 0.2, 5, and 15% sodium hydroxide solutions as successive extracting agents. Fucose, rhamnose, xylose, arabinose, galactose, and galacturonic acid were identified in hemicelluloses purified through copper-complex formation by paper and column chromatographies and crystalline derivatives.
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  • L-Homoserine Fermentation
    Part VI. Effect of Threonine and Methionine on L-Homoserine in Dehydrogenase in Micrococcus glutamicus 534-Co147
    Takashi NARA, Hirotoshi SAMEJIMA, Chuzo FUJITA, Minoru ITO, Kiyoshi NA ...
    1961 Volume 25 Issue 7 Pages 532-541
    Published: 1961
    Released on J-STAGE: February 07, 2011
    DOIhttps://doi.org/10.1271/bbb1961.25.532
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    In the course of studies on “negative feedback control” mechanisms caused by threonineand methionine in L-homoserine fermentation with Micrococcus glutamicus 534-Co147 culture, L-homoserine dehydrogenase was first found to occur in the culture, and effects of variousamino acids on the formation and action of the enzyme were then investigated. The resultwas that L-homoserine dehydrogenase of the culture was repressed by methionine on the onehand, and inhibited by threonine on the other. β-Aspartokinase was found not to be repressedby methionine. From these findings, the sites at which the “negative feedback control” mechanisms work were discussed.
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  • Studies on Allergen of Fasciola hepatica
    Part III. Separation of Allergenic Substances (C5 and P4)
    Kazuyuki MAEKAWA, Masahisa KUSHIBE
    1961 Volume 25 Issue 7 Pages 542-549
    Published: 1961
    Released on J-STAGE: February 07, 2011
    DOIhttps://doi.org/10.1271/bbb1961.25.542
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    Two allergenic substances (C5 and P4), which consisted of ribonucleic acid as their main component, were isolated from the heated extract of liver fluke (Fasciola hepatica) by means of precipitation by ammonium sulphate and phenol extraction, followed by extraction with potassium acetate and ethanol fractionation.
    One of these substances, C5, which was extracted with phenol was electrophoretically homogeneous, but ultracentrifugally was shown to contain one other substance in a small quantity.
    The other substance, P4, which was isolated from the phenol insoluble fraction was electrophoretically and ultracentrifugally homogeneous.
    Both of these were almost the same in biological activity, and their solutions, which were diluted 1: 100, 000 with Ringer's solution, were still active in the intradermal reaction to the fascioliasis of cattle. Judging from their activity and yield, it seems that all the allergenic substances which are in the heated extract of liver fluke, are represented by the protein allergen reported already, as well as by C5 and P4 described here.
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  • Studies on Allergen of Fasciola hepatica
    Part IV. Composition of Allergen P 4
    Kazuyuki MAEKAWA, Masahisa KUSHIBE
    1961 Volume 25 Issue 7 Pages 550-552
    Published: 1961
    Released on J-STAGE: February 07, 2011
    DOIhttps://doi.org/10.1271/bbb1961.25.550
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    This paper deals with the composition of an allergenic substance which was isolated fromliver fluke by means of phenol extraction and other methods. The preparation is composedof ribonucleic acid (95%) as its main component and a small amount of peptide (4.6%).The ribonucleic acid consists of nearly equal parts of adenylic, guanylic, uridylic and cytidylicacids, and the peptide is made up of aspartic acid, glutamic acid, glycine, cystine, alanine, serine, threonine, valine, leucine, histidine, lysine, and arginine.
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  • Studies on L-Glutamic Acid Fermentation
    Part IV. Metabolism of L-Glutamic Acid and Glycolytic Enzymes of the Embden-Meyerhof-Parnas Pathway in Cell-free Extracts of Brevibacterium divaricatum nov. sp.
    Yuan-Chi Su, Nobumasa TANAKA, Koichi YAMADA
    1961 Volume 25 Issue 7 Pages 553-558
    Published: 1961
    Released on J-STAGE: February 07, 2011
    DOIhttps://doi.org/10.1271/bbb1961.25.553
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    The authors have carried out a series of studies on L-glutamic acid fermentation with astrain of Brevibacterium divaricatum nov. sp. in the previous papers.
    In this paper, some metabolism of L-glutamic acid and oxidative decomposition of severalorganic acids concerning the tricarboxylic acid cycle by the resting cells have been studied.The results suggest that L-glutamic acid is one of the final fermentative products of thisbacterium, and the tricarboxylic acid cycle is working as a glutamic acid forming cycle.
    The presence of glucokinase, phosphoglucoisomerase, phosphofructokinase, aldolase, DPNlinkedglyceraldehyde-3-phosphate dehydrogenase, and TPN-linked glucose-6-phosphate dehydrogenase in cell-free extracts of this bacterium was also demonstrated.
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  • Transformations of Cardio-active Steroids by Microorganisms
    Part II. Influence of pH on the Bioconversions of Cardiac Aglycones byRhizopus arrhizus andMucor parasiticus
    Yoshio NOZAKI
    1961 Volume 25 Issue 7 Pages 559-565
    Published: 1961
    Released on J-STAGE: February 07, 2011
    DOIhttps://doi.org/10.1271/bbb1961.25.559
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    The pH of fungal fermentation beer was found to have considerable effect on the microbial transformation of cardiac aglycones. When digitoxigenin and 3-dehydrodigitoxigenin were shaken with acid fermentation beer of Rhizopus arrhizus, 7β-hydroxydigitoxigenin and 3-epi-7β-hydroxydigitoxigenin were obtained as principal dissimilation products, respectively. Upon incubation with the fermentation beer of elevated pH, these substrates were led to the common product, 3-dehydro-7β-hydroxydigitoxigenin. When digitoxigenin was bioconverted with acid and alkaline fermentation beers of Mucor parastiticus, there resulted in the formation of 7β-hydroxydigitoxigenin and of 3-dehydrodigitoxigenin and anhydroperiplogenone respectively, both accompanied with significant amount of periplogenin. With 3-dehydrodigitoxigenin as a substrate, digitoxigenin and 3-epi-digitoxigenin were shown to be present in acid fermentation beer of the same organism.
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  • Studies on the Mode of Action of Dipterex
    Part II. New Glucuronides Obtained from the Urine of Rabbit following Administration of Dipterex
    Junshi MIYAMOTO
    1961 Volume 25 Issue 7 Pages 566-572
    Published: 1961
    Released on J-STAGE: February 07, 2011
    DOIhttps://doi.org/10.1271/bbb1961.25.566
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    To elucidate the reason of low mammalian toxicity of Dipterex, decomposition of this compound by rabbit tissue was investigated. Though splitting of P-C linkage was not confirmed, two glucuronides, presumably derived from the metabolite (s) of Dipterex, were isolated from the urine. Molar ratio of phosphorus and glucuronic acid in them are both estimated as 1:1, and they are observed to differ from trichloroethyl glucuronide. The rapid detoxification might be ascribed to the capacity of glucuronide formation as well as the enzymatic hydrolysis of DDVP, the active ingredient of Dipterex.
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  • Studies on Growth Inhibition of Hiochi-bacteria, Specific Saprophytes of Sake
    Part. VI Muta-aspergillic Acid as a New Growth Inhibitant against Hiochi-bacteria
    Seiji NAKAMURA, Teruo SHIRO
    1961 Volume 25 Issue 7 Pages 573-579
    Published: 1961
    Released on J-STAGE: February 07, 2011
    DOIhttps://doi.org/10.1271/bbb1961.25.573
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    A new growth inhibitant against hiochi-bacteria, C11H18N2O, m.p. 173-4°C, named muta-aspergillic acid, has been isolated from the crystals of crude hydroxyaspergillic acid, obtained from culture filtrate of Asp. oryzae. Successful separation of these two compounds from each other was accomplished by counter current distribution method. The physical and chemical properties of muta-aspergillic acid as well as its physiological properties are described.
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  • Fundamental Studies on the Aerobic Fermentation
    Part VIII. Oxygen Transfer within a Mold Pellet
    Toshimasa YANO, Tôru KODAMA, Kôichi YAMADA
    1961 Volume 25 Issue 7 Pages 580-584
    Published: 1961
    Released on J-STAGE: February 07, 2011
    DOIhttps://doi.org/10.1271/bbb1961.25.580
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    The oxygen uptake rate of aggregated mycelia is decreased to an extent which, in the case of a typical spherical aggregate, could be estimated depending on its diameter, mycelial density, oxygen diffusivity, and so forth. Equations were presented in this paper to evaluate the oxygen uptake rate of an mold pellet. A favorable agreement was found between the calculation and the experiment.
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  • Synthesis and Properties of 6-Deoxy-6-mercapto-D-glucosamine
    Teiichirô ITO
    1961 Volume 25 Issue 7 Pages 585-587
    Published: 1961
    Released on J-STAGE: February 07, 2011
    DOIhttps://doi.org/10.1271/bbb1961.25.585
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    6-Deoxy-6-mercapto-α-D-glucosamine hydrochloride was synthesized from N-anisylidene-1, 3, 4-tri-O-acetyl-6-O-tosyl-β-D-glucosamine, and some of its properties were compared with those of α-D-glucosamine hydrochloride.
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  • Studies on the Sunlight Flavor of Beer
    Part VIII. Mechanism of the Formation of Sunlight Flavor
    Yataro OBATA, Mitsuo KOSHIKA, Haruo TANAKA
    1961 Volume 25 Issue 7 Pages 588-593
    Published: 1961
    Released on J-STAGE: February 07, 2011
    DOIhttps://doi.org/10.1271/bbb1961.25.588
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    Three synthetic lupulone analogues, i. e., 1-acetyl-3, 3, 5-tri-allyl-, 1-acetyl-3, 3, 5-tri-n-propyland 1-acetyl-3, 3, 5-tri-n-butyl-hexadiene-(1, 5)-diol-(2, 6)-on-(4) did not cause sunlight flavor of beer when they were added to a fermented solution of sucrose and exposed to sunlight.
    When methanol solutions of humulone and acetolupuphenonen were exposed to sunlight, changes in the UV-absorption spectra were observed. This fact indicates that humulone and acetolupuphenone underwent some photochemical reaction upon exposure to sunlight.
    Prenyl (γ, γ-dimethylallyl) mercaptan was synthesized, which gave a typical sunlight flavor when it was added, in a trace amount, to a fermented solution of sucrose.
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  • Isolation of Lysergol, Lysergene and Lysergine from the Saprophytic Cultures of Ergot Fungi
    Matazô ABE, Saburô YAMATODANI, Tôgo YAMANO, Mitsugi ...
    1961 Volume 25 Issue 7 Pages 594-595
    Published: 1961
    Released on J-STAGE: February 07, 2011
    DOIhttps://doi.org/10.1271/bbb1961.25.594
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  • ERRATA
    1961 Volume 25 Issue 7 Pages e1
    Published: 1961
    Released on J-STAGE: February 07, 2011
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