Comparisons of proteins synthesized by the sexually differentiated and non-differentiated cells of
Tremella mesenterica IFO-9313 (mating type
ab) were performed with the soluble cell-fraction prepared by centrifugation at 25, 000×g for 30min. By employing the two-dimensional gel electrophoresis, it was demonstrated that alteration in synthesis of some cellular proteins is induced in response to a mating hormone, tremerogen A-10, secreted by
T. mesenterica IFO-9310 (mating type
AB).
Typical example of alteration is as follows: Cellular proteins
a (MW, 30, 000) and
b (MW, 13, 000), located by a region of an isoelectric point, around 5.8, are remarkably decreased and increased, respectively, during sexual differentiation. These findings suggest the occurrence of gene control for sexual differentiation in response to the extracellular information substance.
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