It was strongly suggested that a new bacterial species,
Kluyvera citrophila nov. sp., which has been reported by Asai
et al. as a strain producing a large amount of α-Ketoglutaric acid from glucose, formed this acid via gluconic acid, and perhaps 2-ketogluconic acid, pyruvic acid and the TCA cycle members in the terminal oxidation route, by the following experiments: (1) the Warburg manometric observation on oxygen uptake and carbon dioxide evolution with members of the TCA cycle by intact cells and cell-free extracts, (2) identification of α-Ketoglutaric acid from various substrates such as glucose, gluconic-, 2-ketogluconic-, pyruvic acids and the members of the TCA cycle, by intact cells, (3) detection of C
14 incorporation to α-Ketoglutaric acid from C
14O
2 and glucose or pyruvic acid, and (4) the experiment on the distribution of incorporated C
14 in α-Ketoglutaric acid by the degradation method with potassium permanganate.
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