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Volume 17 (1988)
- Issue 6 Pages 1637-
- Issue 5 Pages 1559-
- Issue 4 Pages 1459-
- Issue 3 Pages 817-
- Issue 2 Pages 401-
- Issue 1 Pages 1-
Volume 17, Issue 5
Displaying 1-10 of 10 articles from this issue
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[in Japanese]1988 Volume 17 Issue 5 Pages 1559
Published: October 15, 1988
Released on J-STAGE: October 07, 2011
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S. SHIOZAKI, K. SAKAGAMI, K. ORITA1988 Volume 17 Issue 5 Pages 1561-1570
Published: October 15, 1988
Released on J-STAGE: October 07, 2011
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A. SUGITACHI1988 Volume 17 Issue 5 Pages 1571-1577
Published: October 15, 1988
Released on J-STAGE: October 07, 2011
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S. TAKATANI1988 Volume 17 Issue 5 Pages 1578-1585
Published: October 15, 1988
Released on J-STAGE: October 07, 2011
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Y. NOISHIKI1988 Volume 17 Issue 5 Pages 1586-1592
Published: October 15, 1988
Released on J-STAGE: October 07, 2011
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Study on selective removal of free-hemoglobinK. MATSUSAKI1988 Volume 17 Issue 5 Pages 1593-1602
Published: October 15, 1988
Released on J-STAGE: October 07, 2011
JOURNAL FREE ACCESSAlthough albumin (Alb) and free-hemoglobin (F-Hb) have almost the same molecular weight, their configurations are slightly different. The new EVAL Filter, 1A with membrane pores of intermediate diameter between Alb and F-Hb selectively removes F-Hb, an excellent characteristic not found in the conventional devices: The sieving coefficients (SC) of Alb and F-Hb were 17.6±6.4% and 36.2±7.2%, the SC of F-Hb being about 2 times higher than that of Alb. The EVAL Filter 1A was clinically employed in 30 cases immediately after cardiopulmonary bypass (CPB), with a subsequent increase in the hematocrit level from 25.7±5.0% to 39.5±6.3%. The total plasma protein was also effectively concentrated from 3.8±1.0g/dl to 6.6±1.7g/dl. The new EVAL Filter also proved to be very useful when emproyed during CPB, especially in one case of severe hemolysis complicated by renal insufficiency, where 5, 960ml of excess water and a total of 1, 907mg of F-Hb could be removed in 146 minutes. Electron microscopically, the membrane of the EVAL Filter revealed an intricate and labyrinthine structure with pores of non-uniform size and configuration. Such a special structure and adequate pore size of the membrane seemed to make selective filtration possible on account of the slight physical differences between the Alb and F-Hb molecules.View full abstractDownload PDF (8234K) -
R. TABATA, A. MORI, T. MAGARA, S. WATARIDA, M. ONOE1988 Volume 17 Issue 5 Pages 1603-1608
Published: October 15, 1988
Released on J-STAGE: October 07, 2011
JOURNAL FREE ACCESSAn exploration was made of an anastomotic insufficiency following revasculation using the expanded polytetrafluoroethylene (EPTFE) vascular prosthesis. The EPTFE vascular prosthesis is made of polytetrafluoroethylene (PTFE) fibers (ca. 0.5μm) which are lined up in the direction of the long axis and bundled at the nodules. Hence, the mechanical properties of the EPTFE vascular prosthesis are specific to a certain direction; tensile force along the long axis direction due to a suture tends to tear the vascular wall of the prosthesis (partly because the suture is pulled parallel to the fiber bundle), while the prosthesis is very strong against tensile force in the circumferential direction (because the tensile force is applied perpendicular to the fiber bundle). When the distance from the cut edge of the EPTFE vascular prosthesis to the suture hole is less than 2mm, the strength of the vascular prosthesis against tensile force (along the long axis direction) depends on the distance; it increases as the distance did. At distances longer than 2mm from the edge, the tensile strength is constant. These results indicate that a suture should be placed at a point sufficiently distant from the cut edge of the prosthesis and a reinforced membrane is effective against tensile force (along the axis direction) applied at the cut edge of this prosthesis, but has the danger of peeling off if care is not taken.View full abstractDownload PDF (8130K) -
Y. MOROHOSHI, M. KAN, T. MAEYAMA, T. OHKUMA, H. SASAKI, M. ICHIKI, A. ...1988 Volume 17 Issue 5 Pages 1609-1620
Published: October 15, 1988
Released on J-STAGE: October 07, 2011
JOURNAL FREE ACCESSCanine vascular endothelial cells (CECs) were obtained by collagenase perfusion from the external jugular vein and cultured serially for the purpose of applying to the prostheses covered with endothelial cells (cultured graft). We cultured the CECs on ePTFE sheets and determined their growth requirements. On native culture dishes, CECs grew rapidly in nutrient media supplemented with 10% fetal bovine serum, EGF (10ng/ml), insulin (1ng/ml), transferrin (10μg/ml), acidic fibroblast growth factor (aFGF, 20ng/ml) and heparin (25μg/ml). The aFGF was essential for CECs' growth and type 1 collagen coating of culture dishes enhanced their proliferation. When culture on ePTFE sheets, CECs were neither able to adhere nor grow on ePTFE alone (the rate of attachement 4.5%). However, they adhered and grew well on collagen coated sheets. The optimal concentration of collagen was 25μg/ml, the rate of attachement was 30-50%, and the doubling time was 30-35 hours. When inoculated at 180cells/mm2, CECs grew to confluence after around 5 day-incubation in culture, and scanning electron microscopy and light microscopy showed CECs monolayer on the ePTFE sheets. Thus, ePTFE sheets coated with collagen might be useful as a material to make a vascular graft covered with endothelial cells. We showed here the basic conditions for the making of cultured tube graft.View full abstractDownload PDF (16822K) -
M. MIYAZAKI, S. SHIOZAKI, S. UCHIDA, K. KAWAMURA, K. SAKAGAMI, Y. KOKU ...1988 Volume 17 Issue 5 Pages 1621-1627
Published: October 15, 1988
Released on J-STAGE: October 07, 2011
JOURNAL FREE ACCESSAn evaluation of dialyzers was performed to realize the fundamental difference between the two groupes of dialyzers available in Japan, 5 types of dialyzer assembled with so-called high-performance membrane (HPM-dialyzers) and 8 types of conventional dialysis membrane (CDM-dialyzer). In each dialyzer, NaCl-clearance and ultrafiltration ratio (UFR) were examined in vitro as well as sieving co-efficient (%SC) for bioactive substances including hormones, albumin, immunoglobulins and β2 microglobulin (BMG) ex vivo. HPM-dialyzers revealed 14% lower clearance and 6 times higher UFR than those of CDM-dialysers. In HPM-dialyzer, the %SC for BMG was high enough ranging from 38.2% to 64.0% except one type assembled with PMMA, however, their high permeability allowed many hormones with molecular weight from 272 to 36, 000 to leak out by innegligible quantities. Loss of albumin was much more in HPM-dialyzers. In conclusion, so-called HPM-dialyzers can be characterized by their low clearance, high UFR and high permeability for low molecular weight proteins as BMG and moreover many hormones. On clinical application of HPM-dialyzers, the drawback of albumin leakage and loss of bioactive substances such as many hormones should be fully evaluated as well as the prospective benefit on removing BMG.View full abstractDownload PDF (1210K) -
[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...1988 Volume 17 Issue 5 Pages 1628-1632
Published: October 15, 1988
Released on J-STAGE: October 07, 2011
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