Simian virus 40 (SV40) was first detected by Sweet and Hilleman (1960 a, b) as a vacuolating agent observable in African green monkey (
Cercopithecs aethiops) kidney cells. This virus has been isolated from some kinds of virus stocks, vaccines and other tissue culture fluids mostly prepared from rhesus monkey kidney cultures. Since SV40 was found to be oncogenic when inoculated into newborn hamsters (Eddy
et al., 1962 a, Gerber
et al. 1962, and Girardi
et al., 1962), a considerable number of studies have already been reported during the last two years. More recently Shein
et al. (1962 a, b, c) and Koprowski
et al. (1962) reported that some kinds of human primary cells could be infected with SV40, being followed by the
in vitro transformation of infected cells.
In our laboratory studies on the growth of SV40 have been carried out, employing primary and continuous African green monkey kidney cells and human continuous cell lines, such as FL, HEp-2 and HeLa-S3, and the present communication deals with the results obtained by the morphological observations of the infected cultures stained with hematoxylin-eosin and fluorescent antibody, and by the infectivity titration based on the specific cytopathic effect.
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