A genetic locus named
cri, which enhanced the expression of
ipa genes, was cloned into
Escherichia coli K-12 from
Shigella flexneri 1b chromosomal DNA. Subcloning and Tn5-Tc1 transposon experiments showed that
cri locus was located on a 2.6-kb
HindIII fragment. Nucleotide sequence analysis of the region revealed at least three open reading frames (ORF), one of which, named
criR, encoded a protein of 226 amino-acid residues and transcriptionally increased the
ipaB expression. The deduced regulatory protein CriR shared a significant homology with bacterial transcriptional activators of the two-component signal transduction family. A homologue of the
criR gene was present in genomic DNA of
Shigella spp. and
E. coli strains, and mapped at the 14.6-min region of
E. coli K-12 chromosomal DNA. These results indicate that
criR is a new member of response regulators.
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