巻号一覧
10 巻 (1977)
- 4 号 p. 407-
- 3 号 p. 271-
- 2 号 p. 161-
- 1 号 p. 1-
10 巻, 4 号
選択された号の論文の10件中1～10を表示しています
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L. J. RASHAN, N. S. GORGEES1977 年 10 巻 4 号 p. 407-412
発行日: 1977年
公開日: 2009/10/28
ジャーナル フリーThe neurosecretory material of the giant cells, with and without axons, in the thoracic ganglion of the fresh-water crab Potamon magnum magnum, was studied. The results showed that the mucosubstances present in the secretory material in both cell types are acidic sulphated mucins. Further, the alcian blue-PAS test showed that the secretion is made up of periodate-reactive and alcianophilic components. The different alcian blue techniques and benzidine reaction used supported the presence of strongly acidic sulphated mucins.抄録全体を表示PDF形式でダウンロード (2752K) -
FUMIE SASAKI1977 年 10 巻 4 号 p. 413-425
発行日: 1977年
公開日: 2009/10/28
ジャーナル フリーEvidence is presented for the existence of two different types of striated muscle fiber from the middle and terminal portions of the tails of the tadpoles Rana catesbeiana, Rana japonica and Xenopus laevis, which were investigated with relation to their histochemical and biochemical properties. The first type of muscle fibers lies immediately below the pigment cells of the skin (peripheral muscle fiber, abbreviated to p.m. fiber); these are of smaller diameter and are abundant in sarcoplasm and mitochondria. The other type (inner muscle fiber, abbreviated to i.m. fiber) lies deeper than to the p.m. fibers; they are of relatively large diameter but contain less sarcoplasm and mitochondria. The intense histochemical localization of lactic dehydrogenase (LDH) activity in the p.m. fibers of the middle and terminal regions of the tail of tadpoles are correlated with the appropriate electrophoretic LDH isozyme patterns. All the zymograms of LDH have been observed to occur faintly at later metamorphic stages.抄録全体を表示PDF形式でダウンロード (18683K) -
YOSHIAKI KINOUCHI, MASA-OKI YAMADA1977 年 10 巻 4 号 p. 426-442
発行日: 1977年
公開日: 2009/10/28
ジャーナル フリーFor studies on the intracellular structure of DNA, the binding of ethidium bromide (EB) to isolated DNA and chromatin was studied by examining their fluorescence spectra in the absence and presence of sodium chloride (NaCl) or ethylenediaminetetraacetic acid (EDTA). Rat liver chromatin of a natural type showing an A240/A260 ratio of 80% and a F450/F330 ratio of about 20% was prepared with a modified version of Marushige and Bonner's method. Both DNA-EB and chromatin-EB showed two excitation peaks at wavelengths of 300 and 330nm and emissions at 585 and 600nm. The relative fluorescence at 585nm (RF585) and the ratio of the RF585 values on excitations at 300 and 330nm (F-ratio) were measured.
With the addition of 0.6M NaCl or 25mM EDTA the RF585 of both DNA-EB and chronation-EB decreased. The decrease caused by NaCl was recovered by removal of NaCl. NaCl and EDTA had a specific effect on the fluorescence of chromatin-EB excited at 300nm but not on that of DNA-EB. With increase in the NaCl concentration, the F-ratio of chromatin-EB increased to a maximum at 0.15M NaCl and then decreased at higher salt concentrations. The optimal conditions for fluorometry of the chromatin were found to be in the presence of 0.15M NaCl and low concentrations of EDTA (less than 3mM) and EB (3-10mM).抄録全体を表示PDF形式でダウンロード (1264K) -
MASARU FUKUDA, TAKUJI ISEMURA, NAOYUKI MARUO, KAZUO NAKANISHI, NORBERT ...1977 年 10 巻 4 号 p. 443-452
発行日: 1977年
公開日: 2009/10/28
ジャーナル フリーStandardization and improvements upon a previously reported method (Fukuda et al. 1975) for the cytofluorometric quantitation of the hemoglobin (Hb) and nuclear DNA contents of a single erythroid cell were described. Intracellular Hb was converted to fluorescent porphyrin by irradiation with violet light (λ 405nm, fluence rate, 12×10-2mJ/sec/mm2) in the presence of SH-donor (mercaptoethylamine hydrochloride, MEA). The intensity of porphyrin fluorescence increased with prolonged photochemical reaction reaching a plateau level at 40min (total 288mJ/mm2). With this improved method, maximum fluorescence for erythroid cells was found to be about 15 times stronger in intensity than with the previously reported method.
Under maximum conditions a linear relationship was found between fluorescence intensity and Hb content (MCH) through the use of the peripheral erythrocytes of different adult male animals (sheep, man, and chick).
The fluorescences of porphyrin and Feulgen DNA were measured without interference with each other, through analysis of the emission spectra on a erythroblast lightly stained with pararosaniline Feulgen after the optimum photochemical reaction.抄録全体を表示PDF形式でダウンロード (5331K) -
SHIGEAKI KOBAYASHI1977 年 10 巻 4 号 p. 453-470
発行日: 1977年
公開日: 2009/10/28
ジャーナル フリーIn order to elucidate the effect of stimulation of the cervical sympathetic nerve on catecholamine (CA) fluorescence of the cerebral arteries, unilateral superior cervical ganglia (SCG) of 18 adult cats were stimulated under pentobarbital anesthesia while 15 cats were used as controls. Short segments of the large arteries of the cerebral base were taken out immediately after various periods of electrical stimulation, processed through Falck-Hillarp's method, and embedded in paraffin, then 8μ thick cross sections were serially cut. Fluorescence photomicrographs were taken on both sides of the respective vessels and the intensities of CA fluorescence were compared with each other. The results obtained were as follows:
1. CA fluorescence was identified in the cerebral vascular walls of the controlled animals and there was no significant difference in CA fluorescence observed between the two sides of the respective vessels.
2. In the experimental animals, CA fluorescence was identified on both sides and it generally increased in the vascular walls of the stimulated side as compared with the unstimulated side. The increase of CA fluorescence on the stimulated side was prominent in the internal carotid arteries, while, it was noted least marked in the posterior cerebral arteries.抄録全体を表示PDF形式でダウンロード (14361K) -
R. YOSHIYUKI OSAMURA, KEIICHI WATANABE1977 年 10 巻 4 号 p. 471-477
発行日: 1977年
公開日: 2009/10/28
ジャーナル フリーThe functional differentiation of the posterior limb of Rathke's pouch in the human fetal pituitaries of gestational age ranging from 5 weeks to 24 weeks was studied by the peroxidase-labeled antibody method, using antibodies against ACTH, ACTH17-39, MSH, GH, PRL, TSH and LH. Our results indicated that ACTH was the first hormone localized in the posterior limb at 5 weeks, and the posterior limb possessed the potentiality of the differentiation to MSH, GH, PRL and LH secreting cells. However, ACTH and MSH remained as the predominant hormone secreted by the posterior limb cells. Possible mechanism to promote ACTH and MSH secreting cells is also discussed.抄録全体を表示PDF形式でダウンロード (12126K) -
MIHIR K. DUTT1977 年 10 巻 4 号 p. 478-482
発行日: 1977年
公開日: 2009/10/28
ジャーナル フリーTreatment of sections with 9N sulphuric acid at 5°C for 1hr, 18N nitric acid at 60°C for 10min or with 1N or 6N hydrochloric acid at 60°C for 40 and 20min respectively brings about the complete removal of RNA from formalinfixed mammalian tissues as is indicated by methyl green and pyronin staining. Similarly treated sections, when stained with an aqueous solution of toluidine blue O at pH3.0 reveal blue nuclei only, indicating total loss of cytoplasmic basophilia.抄録全体を表示PDF形式でダウンロード (482K) -
KAZUMASA HOSHINO, GUNTER KIEFER1977 年 10 巻 4 号 p. 483-493
発行日: 1977年
公開日: 2009/10/28
ジャーナル フリーA newly modified triple stain for DNA with Feulgen reaction using azur B-Schiff reagent, for polysaccharides with PAS reaction using pararosanilin-Schiff reagent, and for proteins with naphthol yellow S facilitates not only cytophotometric study of these three important tissue components utilizing three different wavelengths, but enables identification of at least five different types of submandibular gland cells as well. These cell types include the cells in the acinus, the convoluted secretory tubule, the intercalated duct, and the striated excretory duct; also a “peculiar” cell which is described herein for the first time. Preliminary investigations using cultured L cells revealed that azur B which was once bound to DNA was partially replaced by pararosanilin Schiff reagent, when the latter was subsequently applied. However, when the sequence of the two Schiff reagents was reversed, such a phenomenon was not observed. The color differentiation of the present triple stain was most distinct after glutaraldehyde fixation and least so after acid alcohol fixation. This stain may be applied following embedding in either paraffin, methacrylate, or plastic, and also in isolated single cell preparations. A somewhat improved method of separating single cells from the mouse submandibular gland is also described.抄録全体を表示PDF形式でダウンロード (3832K) -
I. NAGATSU, Y. KONDO, S. INAGAKI, N. KARASAWA, T. KATO, T. NAGATSU1977 年 10 巻 4 号 p. 494-499
発行日: 1977年
公開日: 2009/10/28
ジャーナル フリーSpecific antiserum to bovine adrenal tyrosine hydroxylase (TH) was produced in rabbits, and applied to the immunofluorescent study of the enzyme. Specific immunofluorescence for the enzyme was observed in guinea pig and bovine adrenal medulla and locus coeruleus of rats.
Immunofluorescent pictures of TH accumulated in the sciatic nerves of rats and guinea pigs after ligation were first shown in this study. The morphological localizations of TH were compared with biochemical ones and both localizations were in close agreement. Retrograde axoplasmic transport of TH was shown by immunofluorescence on the first day after ligation. The comparison between. TH and dopamine β-hydroxylase (DBH) was also made from the point of view of their localizations in vivo by immunofluorescence.抄録全体を表示PDF形式でダウンロード (6380K) -
TOMOYUKI HARADA, HAJIME SUGIHARA, HIDEO TSUCHIYAMA, HIROYUKI NODA1977 年 10 巻 4 号 p. 500-507
発行日: 1977年
公開日: 2009/10/28
ジャーナル フリーPigmented lipid histiocytes (PLH) of chronic granulomatous disease (CGD) were examined by histochemistry and electron microscopy. The yellowish brown pigments were stainable with Sudan III and IV even in paraffin sections, and the sudanophilia was kept after oxidation with peracetic acid followed by methylation. In addition, the granules were positive to Gomori's chromium hematoxylin stain, 0.02% Nile blue sulfate stain and leuco-malachite green stain. Ultrastructural observation showed numerous intracytoplasmic granules in variable shapes and sizes measuring up to 4.8μ in diameter, with various electron densities. From these results, the pigment in PLH of CGD is comparable to a ceroid-like substance. The nature of the pigment and the pathogenesis of PLH are also discussed.抄録全体を表示PDF形式でダウンロード (7114K)
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