巻号一覧
22 巻 (1989)
- 6 号 p. 593-
- 5 号 p. 491-
- 4 号 p. 411-
- 3 号 p. 295-
- 2 号 p. 161-
- 1 号 p. 1-
22 巻, 2 号
選択された号の論文の12件中1～12を表示しています
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MING HU HAN, YING JIE PIAO, DE WAN GUO, KAZUO OGAWA1989 年 22 巻 2 号 p. 161-172
発行日: 1989年
公開日: 2010/02/26
ジャーナル フリーAxonal changes, in particular the breakdown and removal of the myelin sheath, were studied using morphological method and cytochemical technique in the sciatic nerves of rats following transection. The details of axonal loss were examined during the first few days after transection. Axonal degradation was immediately accompanied by the fragmentation of the myelin sheath. It was found that: (a) a part of the myelin sheath separated from Schwann cells and smashed into debris, then the myelin debris was phagocytosed and digested by macrophages at endoneurial space; the others, most of the myelin sheath, remained within Schwann cells until being digested; (b) both small debris of myelin sheath (SDMS) and large membrane-bound myelin debris (LMBMD) were found in Schwann cells; acid phosphatase (AcPase) activity was detected in SDMS earlier than in LMBMD; (c) macrophages were found in myelin sheath tubes; they participated in removing a part of degenerating axons. Observation confirmed that Schwann cells had no ability to actively phagocytose myelin, that the majority of myelin debris were removed by Schwann cells, and that the minority of them were dealt with by macrophages. The ability of Schwann cells to digest myelin debris suggested that Schwann cells play a crucial role in vivo in removing myelin debris and that the process of myelin debris in Schwann cells might be significant for their mitotic activity.抄録全体を表示PDF形式でダウンロード (2992K) -
KAZUTO YAMADA, HIROYUKI SHINOHARA, TAKAAKI TANAKA, YUKIO OKADA, MASAHI ...1989 年 22 巻 2 号 p. 173-186
発行日: 1989年
公開日: 2010/02/26
ジャーナル フリーEighty pleomorphic adenomas of human salivary glands were examined by the immunoperoxidase method using a variety of monoclonal antibodies specific for certain of the cytokeratins. Expressions of monoclonal antibodies against KL1, PKK1, K8.13, K8.60, K4.62, RPN1164 and RPN1165 were observed in luminal tumor cells of pleomorphic adenomas, as well as in intercalated and striated duct cells of the normal salivary glands, while monoclonal antibody K8.12 was reacted to outer tumor cells, as well as ductal basal cells in the normal glands. According to keratin existence with the use of monoclonal antibodies, outer tumor cells of tubulo-ductal structures contained keratin nos. 16 and 13, and luminal tumor cells nos. 19, 18, 11, 10 and 8, Great heterogeneities of keratin distribution were given in outer and luminal tumor cells of tubulo-ductal and duct-like structures. It is postulated that stem cells may be present in intercalated ductal segments and they differentiate to both ductal basal cells or intercalated ductal cells, and two cells showed different expressions for keratins. Pleomorphic adenoma might arise from those two progenitor cells, is suspected. The presence of reserve cells may coincide with progenitor cells as ductal basal cells, and ductal basal cells may differentiate to outer tumor cells and also modified myoepitheial cells.抄録全体を表示PDF形式でダウンロード (3435K) -
SHEN-QIU LUO, MASAHIRO SAKAI, CI-SHENG ZHONG, KAZUO OGAWA1989 年 22 巻 2 号 p. 187-197
発行日: 1989年
公開日: 2010/02/26
ジャーナル フリーThe neutral pH p-nitrophenyl phosphatase activity (p-NPPase) on the lysosomal membrane in rat liver cells was demonstrated histo- and cytochemically. The enzyme activity was observed mainly on the lysosomal membrane surfaces and its matrices by using the original incubation medium consisting of 5 mM Mg salt p-NPP, 50 mM KCl, 2.5 mM levamisole, 25% DMSO and 2 mM lead citrate in 25 mM Tricine-KOH buffer, pH 7.3. When an inhibitor of acid phosphatase (5-10 mM, NaF) was added to the incubation medium, the enzyme activity on lysosomal matrices was inhibited but the activity on membranes was not. Either 4, 4-diisothiocyanatostilbene-2, 2′-disulphonic acid (10 mM DIDS) or N-ethylmaleimide (10 mM NEM) in the incubation medium could inhibit the enzyme activity on the lysosomal membrane but not in the matrices. The enzyme activity of both lysosomal membranes and its matrices was inhibited when the medium contained NaF and DIDS, or NaF and NEM at the same time. Furthermore, if the Mg salt p-NPP was replaced by Na salt p-NPP in the NaF containing standard incubation medium, the enzyme activity was not observed at all on lysosomes. These results suggest that the lysosomal membrane p-NPPase activity at neutral pH may represent H+-ATPase activity.抄録全体を表示PDF形式でダウンロード (2933K) -
ANDREAS SCHOBER, WILFRIED SCHOBER, HANS LUPPA1989 年 22 巻 2 号 p. 199-205
発行日: 1989年
公開日: 2010/02/26
ジャーナル フリーWe have investigated the projection of the mesencephalic raphe nuclei on the basal forebrain (BF) by using nerve tracing (HRP) and immunohistochemical methods. The study shows the existence of an ipsilateral projection from the mesencephalic raphe nuclei to the BF. The number of labelled cells differs depending on the HRP-injection site in the BF. The ncl. raphe centralis superior projects more to the rostral nuclei of the BF. Only a minimal part of the mesencephalic raphe neurons projects to the BF. About 50% of these cells are double-labelled. therefore, the projection of raphe nuclei to the BF is realized by a minority of serotoninergic cells.抄録全体を表示PDF形式でダウンロード (1511K) -
HARUHIKO SHINOGAMI1989 年 22 巻 2 号 p. 207-213
発行日: 1989年
公開日: 2010/02/26
ジャーナル フリーThe localization of ouabain sensitive, K+-dependent p-nitrophenylphosphatase (K+-NPPase), a component of the transport adenosine triphosphatase (Na+, K+-ATPase) system, was ultracytochemically investigated in the heminode of Ranvier in the rat optic nerve. K+-NPPase activities were noted on the nodal and paranodal axolemma, and on the paranodal myelin lateral loops. In the nodal region, the axolemma, which lies above the dense cytoplasmic undercoating, showed remarkably strong K+-NPPase activity.抄録全体を表示PDF形式でダウンロード (1541K) -
HIDEYUKI GOTO1989 年 22 巻 2 号 p. 215-225
発行日: 1989年
公開日: 2010/02/26
ジャーナル フリーTissue and organ distribution of radioactive carbon from 14C-labeled arginine in the mouse was studied by whole-body autoradiography and biochemical analysis. The mice injected intravenously with L- [U-14C]arginine were sacrificed at various intervals. Examination of autoradiographs disclosed that the injected 14C-arginine was rapidly taken up from the blood by the organs. The radioactivity in the pancreas was the highest throughout the intervals after injection in this investigation. The general pattern of the autoradiographs obtained after injection of radioactive arginine had a resemblance to those reported about other amino acids.
The comparative values among radioactivity in various organs estimated by a liquid scintillation counter were consistent with those obtained from whole-body autoradiographs. Radioactivity in the acid-insoluble fractions was increased with time in all organs examined. High-performance liquid chromatography of the acid-soluble fractions disclosed that several radioactive substances were detected in the examined organs soon after injection and that the amounts of the radioactive arginine and its metabolites and/or their molar ratios were different among the organs.抄録全体を表示PDF形式でダウンロード (2313K) -
YOTARO KONISHI, YASUYO HATA, KEN FUJIMORI1989 年 22 巻 2 号 p. 227-231
発行日: 1989年
公開日: 2010/02/26
ジャーナル フリーPDF形式でダウンロード (1037K) -
SHINOBU C. FUJITA1989 年 22 巻 2 号 p. 235-242
発行日: 1989年
公開日: 2010/02/26
ジャーナル フリーPDF形式でダウンロード (1330K) -
HIROSHI KIMURA, IKUO TOHYAMA, KOUNOSUKE MIZUTANI, JUNZO OCHI1989 年 22 巻 2 号 p. 243-258
発行日: 1989年
公開日: 2010/02/26
ジャーナル フリーFibronectin immunocytochemistry applied in trypsin-treated sections of embryonic rat brain revealed several positive structures. These include, in 18-day-embryo for example, somata of neuroblasts, ependymal cells, developing nerve terminals, meninges and blood vessels. Without trypsin treatment no positive immunoreactivity was seen in neural elements, though weak staining was observed only in meninges and capillaries. The neuroblasts possessing intracytoplasmic immunoreactivity for fibronectin were distributed in particular brain regions such as the cortical plate of the cerebrum and the primordium of the cerebellar nuclei. The intensity of immunostaining in neuronal and vascular structures gradually decreased or diminished at around perinatal periods. The results suggested that fibronectin may have important roles in neuronal migration or maturation. Moreover, fine positive processes ramified from capillaries suggested the role of fibronectin in neovascularization.抄録全体を表示PDF形式でダウンロード (4779K) -
ENZYMATIC POLARITY IN BRAIN CAPILLARIESKENICHIROU INOMATA, FUMIO NASU, KAZUHIRO TOMIYASU, MOTONOBU MIYAZAKI1989 年 22 巻 2 号 p. 259-264
発行日: 1989年
公開日: 2010/02/26
ジャーナル フリーWe performed ultracytochemical studies of Mg-ATPase and TPPase activities in circumventricular capillaries at the developmental stage of rats, from 17 gestational days to 8 postnatal weeks, comparing with hippocampal capillaries. During the course of perinatal development, the predominant localization of both enzymatic activities, particularly Mg-ATPase, in circumventricular capillaries was shifted from the luminal cell membrane to the antiluminal cell membrane. In all hippocampal capillaries observed, the predominant localization of both enzymatic activities was the antiluminal cell membrane. It was suggested that immaturity of circumventriculr capillaries may be one of the causative factors inducing intraventricular and subependymal hemorrhages in neonates.抄録全体を表示PDF形式でダウンロード (1588K) -
CHIZUKA IDE, KOUJIRO TOHYAMA, TATSUO USHIKI, TOHRU NITATORI, YASUYO YO ...1989 年 22 巻 2 号 p. 265-274
発行日: 1989年
公開日: 2010/02/26
ジャーナル フリーSchwann cell basal laminae of mouse sciatic nerve were studied histochemically using tannic acid (TA) and ruthenium red (RR) in tissue sections. In addition, basal laminae isolated by sonication were examined using ferritin-conjugated lectin for carbohydrate residues and by immunohistochemistry for laminin. Isolated basal laminae were also examined by RR. The outer (interstitial) surface of the basal laminae were observed by scanning electron microscopy (SEM).
By TA staining, the basic lattice-like structure of lamina densa was revealed, and fine and thick filamentous fuzzy structures were seen to project from both sides of this lattice.
By RR staining, there were dark-stained spots of proteoglycans, arranged at 30-100 nm intervals, on the outer surface of the basal lamina. These spots are considered to be primarily responsible for the attachment of basal lamina to the surrounding connective tissue matrix.
Out of 12 lectins examined, WGA, RCA-I and ConA bound to the isolated basal laminae: WGA tended to bind to the inner surface, while the other two lectins bound almost equally to both sides.
Laminin appeared to be more abundant on the inner surface than on the outersurface of the basal lamina.
SEM revealed a somewhat fuzzy but unexpectedly smooth outer surface of basal lamina. There were small pits scattered randomly on the basal lamina.
These findings are discussed from the point of view of the polarity of the basal lamina, and its role in cell adhesion to the surrounding extracellular matrices.抄録全体を表示PDF形式でダウンロード (3027K) -
NOBUYUKI SHITARA, KAZUYUKI KITAMURA, TERUMI WADA, HIROHIKO NAKAMURA, K ...1989 年 22 巻 2 号 p. 275-288
発行日: 1989年
公開日: 2010/02/26
ジャーナル フリーThe ten human cell lines derived from glioma tissues were characterized for the aspects of the phenotypic expression of GFAP and S-100 protein, DNA-ploidy, doublhg time, and response to DBc-AMP and LAK cells. The expression of the TfR was evaluated on these established cell lines with correlation to their phenotypic markers and characteristics. As a result of immunohistochemical studies on frozen materials using a monoclonal anti-Tfit antibody, the high expression of the TfR was related to the doubling time (36-48 hr), but not to the phenotypic expression of glial markers (GFAP and S-100 protein) and other characteristics. And thus, rapidly proliferating astrocytic cell lines including C6 and U251 showed the high level of the TfR in the cells.
For the flow cytometry (FCM), the cell harvest with trypsin abolished the extracellular part of TfR molecules from the cell membrane, and thus, single cell preparation with trypsin was not appropriate for the study. On the other hand, EDTA (0.02%) with mechanical shaking provided a preservation of the TfR, molecules for FCM study. The series of FCM analysis demonstrated that newly synthesized (or recycled) TfR molecules appear in the extracellular matrix of the cell membrane by 12 hr and then increase in quantity up to 28 hr after trypsin digestion.
The results suggest that the TfR molecules are functioning in rapidly proliferating cells derived from the central nervous system as described previously in other types of tissue.抄録全体を表示PDF形式でダウンロード (3035K)
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