巻号一覧
21 巻 (1988)
- 6 号 p. 521-
- 5 号 p. 439-
- 4 号 p. 321-
- 3 号 p. 215-
- 2 号 p. 91-
- 1 号 p. 1-
21 巻, 5 号
選択された号の論文の8件中1～8を表示しています
- |<
- <
- 1
- >
- >|
-
NOBUKAZU ARAKI, TORU NODA, KAZUO OGAWA1988 年 21 巻 5 号 p. 439-453
発行日: 1988年
公開日: 2009/10/28
ジャーナル フリーThe effect of “Sho-saiko-to” (TJ-9), a traditional Chinese medicine, on the D-galactosamine (D-Gal)-induced hepatic injury of rats was estimated from morphological and cytochemical aspects, comparing the methods of administration of TJ-9.
A single administration of D-Gal to rats resulted in a hepatic injury with some morphological and histo-cytochemical changes. Both light and electron microscopy showed a disorder in the hepatic lobular structure and configuration of hepatic cords, retention of lipid droplets in hepatocytes, infiltration of inflammatory cells, a decrease in the number of sinusoidal microvilli, dilatation of bile canaliculi, etc. Cytochemical observation showed a remarkable decline of activities of 5′-nucleotidase (5′Nase), a plasma membranous enzyme, and glucose-6-phosphatase (G6Pase), an endoplasmic membranous enzyme, in the liver cells injured by D-Gal. However, in the rats receiving prophylaxis or therapeutic treatment with TJ-9 by intraperitoneal or oral administration, neither severe morphological changes nor a remarkable decline of the enzyme activities was observed. These results suggest that the prophylaxis with TJ-9 prevents the occurrence of hepatic injury, and therapeutic treatment improves the pathological changes induced by D-Gal. Comparing the methods of TJ-9 administration to the rats, intraperitoneal administration was more effective than oral on the hepatic injury.
Enzyme cytochemical changes in the liver could be regarded as a useful hallmark for estimating the effect of a drug and the degree of hepatic dysfunction, as well as structural changes in the liver tissue. The present study provided morphological and cytochemical evidence supporting the fact that TJ-9 is efficacious in the treatment of human hepatitis.抄録全体を表示PDF形式でダウンロード (17370K) -
AN ENZYME HISTOCHEMICAL STUDYKOICHI YAMAMOTO, YOICHIRO KATO, HIROTAKA MATSUMOTO, SHINICHI MORIYAMA, ...1988 年 21 巻 5 号 p. 455-461
発行日: 1988年
公開日: 2009/10/28
ジャーナル フリーThe histochemical localization of thyroid peroxidase (TPO) and gamma-glutamyl transpeptidase (GGT) in diisopropanolnitrosamine (DIPN) induced rat thyroid lesions was examined. The former was utilized as a marker of the endocrine function of the follicular epithelium and the latter as that of neoplastic growth.
The two enzymes revealed an inverse correlation in their patterns of localization, i. e., TPO was positive in most of the benign nodular lesions and negative in malignant nodules, while GGT was positive in the nodules or areas of nodules which were negative for TPO.抄録全体を表示PDF形式でダウンロード (2618K) -
KOJI KAMI, KENJIRO YASUDA1988 年 21 巻 5 号 p. 463-472
発行日: 1988年
公開日: 2009/10/28
ジャーナル フリーIn laying hen oviduct tissues fixed with buffered-formaldehydes, the definitive localization of endogenous biotin-binding protein and immunogenic avidin was demonstrated in the cytoplasm of tubular gland cells and PAS-positive seromucous cells of the epithelium of the lower third magnum and the isthmus. Smaller amounts of the protein were also associated with tubular gland cells and deep-lining cells as crypt-constituents, excluding epithelial goblet-like cells, of the middle magnum. In biotin-affinity histochemistry, suppression by pre-incubation with biotin was evaluated to be the most satisfactory procedure for positive control stainings. The staining intensity and pattern achieved with biotinylated-horseradish peroxidase was more prominent than that achieved with biotinylated-alkaline phosphatase techniques. Positively stained profiles of tubular gland cells varied considerably with different cellular constituents, having a mosaic-like appearance. In addition, every biotin-binding site was not always stained by immunoperoxidase techniques. This paper discusses the reasons for these heterogeneous localizations resulting from these two staining procedures.抄録全体を表示PDF形式でダウンロード (6736K) -
GERD POEGGEL, HANS LUPPA, ANDREA SCHMIDT1988 年 21 巻 5 号 p. 473-480
発行日: 1988年
公開日: 2009/10/28
ジャーナル フリーCyclic nucleotide phosphodiesterase (PDE) was demonstrated by histochemical methods on the electron microscopic level. The method was based on a phosphate precipitation using cerium ions as the capture metal. The enzymatic reaction could be demonstrated in synapses, on endoplasmic reticulum, microtubules, the nuclear envelope, the cytoplasm and in some axons. Furthermore strong activity was found in glial cells, especially astrocytes. Only little reaction was detectable in the capillary endothelium. Because of the destroying action of the snake venom the morphology of the tissue was poor.抄録全体を表示PDF形式でダウンロード (7798K) -
ION I. MORARU, LUMINITZA CIOTARU, MIOARA MANCIULEA, MIHAI ISAC, CEZAR ...1988 年 21 巻 5 号 p. 481-488
発行日: 1988年
公開日: 2009/10/28
ジャーナル フリーPhospholipase C is a key enzyme for transmembrane signalling, since it generates phosphoinositide-derived intracellular messengers. The enzyme localization was studied by indirect immunofluorescent technique, using a rabbit monospecific antibody directed to the purified bacterial phospholipase C. This antibody, which is inhibitory in vitro and on phospholipase C of intact cells, cross-reacted with the enzyme from a variety of cell types (skeletal and smooth muscle cells, nerve cells, fibroblasts, leukocytes, erythrocytes) and species (frog, guineapig, rat, man). The reaction was specific, as demonstrated by using the F(ab')2 fragment of the antibody molecule. Since the membrane-bound phospholipase C can be revealed by immunofluorescence on the surface of isolated, intact cells, it is concluded that the membrane-associated phospholipase C is a transmembrane protein.抄録全体を表示PDF形式でダウンロード (2679K) -
A STUDY BY IN SITU HYBRIDIZATION USING A BIOTINYLATED PROBE AND STREPTAVIDIN-ALKALINE PHOSPHATASEYUSEI ARAKAKI, HIROSHI TOYODA, SEKIKO WATANABE, SHUJI SEKI, TAKUZO ODA1988 年 21 巻 5 号 p. 489-498
発行日: 1988年
公開日: 2009/10/28
ジャーナル フリーIn situ hybridization using a biotinylated probe and streptavidin-alkaline phosphatase was applied to the detection of hepatitis B virus (HBV)-DNA in for-maimfixed and paraffin-embedded liver-biopsied tissues from 24 patients with chronic type B liver diseases (all carriers of serum HBsAg), and the results were compared with those of the immunohistochemical detection of HBsAg and HBcAg. The specificity of in situ hybridization reactions was confirmed by negative staining of the control tests. HBV-DNA was detected in the sections of 8 cases with serum HBeAg and one case without serum HBeAg, and was located predominantly in the cytoplasm of hepatocytes showing various staining patterns such as diffuse, regional or peripheral. The lobular or pseudolobular distribution of HBV-DNA in the liver tissues was divided into scattered, clustered and diffuse type. The scattered type was observed predominantly in the sections of cases with active liver cirrhosis. As regards HBsAg and HBcAg in the liver tissues, HBsAg was detected in the sections of 18 cases with or without serum HBeAg; 8 were detectable for HBV-DNA by in situ hybridization and 10 were not detectable. HBcAg was detected in the sections of 9 cases all with serum HBeAg; 6 were detectable for HBV-DNA by in situ hybridization and 3 were not detectable. The sections of 5 out of the 6 detectable cases for HBV-DNA revealed the cytoplasmic HBcAg in hepatocytes. These findings suggested that HBV-DNA in hepatocytes detected by in situ hybridization correlated with the presence of serum HBeAg and cytoplasmic HBcAg rather than nuclear HBcAg in hepatocytes in chronic type B liver diseases.抄録全体を表示PDF形式でダウンロード (8334K) -
ROSANNA NANO, RITA REZZANI, LUIGI RODELLA, GIUSEPPE GERZELI1988 年 21 巻 5 号 p. 499-506
発行日: 1988年
公開日: 2009/10/28
ジャーナル フリーThe availability of anti-human dihydrofolate reductase polyclonal antibodies, allowed us to develop an immunohistochemical method improving the tetrazolium salt method for the demonstration of dihydrofolate reductase in samples in peripheral blood and bone marrow. This enzyme, immunohistochemically demonstrated with the alkaline-phosphatase/anti-alkaline-phosphatase (APAAP) method, progressively increased during the normal differentiation of the granulocytopoietic series while it decreased in the erythropoietic series. The lymphocytes showed various positivity patterns which related to their subpopulations. These results were in agreement with previous observations by the tetrazolium salt method. Dihydrofolate reductase may be a useful marker in the study of granulocytic and lymphocytic cell lineages.抄録全体を表示PDF形式でダウンロード (8434K) -
MARK C. WILLINGHAM1988 年 21 巻 5 号 p. 507-518
発行日: 1988年
公開日: 2009/10/28
ジャーナル フリーMorphologic methods offer unique opportunities to study some of the characteristics of malignant and normal cells. One of the promising areas of cancer therapy is the use of targeted toxins, such as immunotoxins, that can selectively destroy tumor cells. We have used morphologic approaches to the screening and evaluation of monoclonal antibodies for use as part of immunotoxins directed against human ovarian cancers. Screening methods that utilize immunoflorescence and immunoperoxidase methods have allowed the rapid isolation of hybridomas that react selectively with tumor cells in tissue culture and in solid tumors, while showing only limited reactivity with important normal tissues. Another area of cancer biology in which morphologic methods have proved useful is the study of multidrug resistance of human tumors. The efflux activity seen in single multidrug resistant cells can be demonstrated morphologically using fluorescent chemotherapeutic drugs, such as daunomycin. The protein responsible for this efflux pump activity, P170, has been localized morphologically to the plasma membrane in individual cultured cells, as well as located using immunohistochemistry in normal liver, kidney, GI tract, adrenal and cerebral capillaries. These locations enhance understanding of the role of this protein in the excretion of toxic hydrophobic compounds, both in chemotherapy and in normal organ physiology. These methods also allow simple and rapid screening techniques to evaluate other drugs that might be able to reverse the multidrug resistance phenotype and render human tumors more sensitive to chemotherapy.抄録全体を表示PDF形式でダウンロード (10891K)
- |<
- <
- 1
- >
- >|