巻号一覧
33 巻 (2000)
- 6 号 p. 405-
- 5 号 p. 319-
- 4 号 p. 235-
- 3 号 p. 153-
- 2 号 p. 63-
- 1 号 p. 1-
33 巻, 3 号
選択された号の論文の12件中1～12を表示しています
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Jun Nakayama, Tsutomu Katsuyama, Minoru Fukuda2000 年 33 巻 3 号 p. 153-157
発行日: 2000年
公開日: 2001/03/31
ジャーナル フリーParadoxical Concanavalin A staining(PCS) is a histochemical method for identifying class III mucin or gastric gland mucous cell−type mucin. This mucin is largely found in mucous neck cells and pyloric gland cells of the gastric mucosa as well as Brunner’s glands of the duodenum. Recently, we have established that the carbohydrate moiety identified by PCS is GlcNAcα 1→4Galβ→R by cloning a glycosyltransferase, α1, 4−N−acetylglucosaminyltransfearase(α4GnT). In this review, we have focused on our cloning strategy of the glycosyltransferase, α4GnT and determination of carbohydrate moiety responsible for class III Con A reactivity.抄録全体を表示PDF形式でダウンロード (487K) -
Takeshi Suzuki, Toshiyuki Matsuzaki, Kuniaki Takata2000 年 33 巻 3 号 p. 159-162
発行日: 2000年
公開日: 2001/03/31
ジャーナル フリーSGLT1, an isoform of Na+−dependent glucose cotransporters, is localized at the apical plasma membrane in the epithelial cells of the small intestine and the kidney, where it plays a pivotal role in the absorption and reabsorption of sugars, respectively. Preferential localization of SGLT1 in the apical plasma membrane domain of these epithelial cells is crucial in the vectorial transfer of sugars. Mutations of SGLT1 gene have been found to be responsible for the congenital glucose galactose malabsorption syndrome. These mutant genes, when expressed in Xenopus oocytes, resulted in the alteration in their cellular localization. In this review, we focus on the molecular mechanism for the localization of SGLT1 in the polarized epithelial cells.抄録全体を表示PDF形式でダウンロード (368K) -
Yoshihiro Akimoto, Gerald W. Hart, Hiroshi Hirano2000 年 33 巻 3 号 p. 163-167
発行日: 2000年
公開日: 2001/03/31
ジャーナル フリーO−GlcNAc transferase(OGT) catalyzes the attachment of N−acetylglucosamine monosaccharides to the hydroxyl group of serine or threonine residues of intracellular proteins and may play an important role in the hexosamine pathway. We examined the localization of OGT protein in the rat pancreas. Immunofluorescence staining with antibody raised against OGT stained both the exocrine acinar cells and endocrine islet cells. The acinar cell nucleus and the zymogen granule region were intensely stained. In the islets of Langerhans, especially in the A cells, intense staining with anti−OGT antibody was observed. Immuno−electron microscopy showed the OGT to be restricted to the euchromatin of the nucleus and around the secretory granules of exocrine acinar cells and endocrine islet cells. These results suggest that OGT is involved in the regulation of transcription and of granular secretion. Thus, O−GlcNAcylated protein(s) may be an important component of the glucose−sensing mechanism in the pancreas.抄録全体を表示PDF形式でダウンロード (452K) -
Tokuko Haraguchi, Takako Koujin, Yasushi Hiraoka2000 年 33 巻 3 号 p. 169-175
発行日: 2000年
公開日: 2001/03/31
ジャーナル フリーThe jellyfish green fluorescent protein(GFP) is a powerful molecular tool to fluorescently label specific proteins by fusing the GFP−coding gene to the gene of interest; the dynamic behavior of these fusion proteins can be examined in living cells. In order to examine the spatial and temporal coordination of the GFP fusion protein with other intracellular structures, we use a time−lapse multiple−wavelength fluorescence microscope system that is capable of recording simultaneously multiple cellular components in the living state. For example, using the system, we have observed dynamic behavior of chromosomes and several GFP fusion proteins−such as lamin B receptor−GFP, cyclin B1−GFP and CENP−B−GFP−in living human cells during mitosis. This cytological technology is also applicable for visualization of mitotic and meiotic events in yeast cells. Thus, live observation of GFP fusion proteins is useful for understanding the full relevance of the temporal and spatial relationships between multiple cellular components.抄録全体を表示PDF形式でダウンロード (465K) -
Atsuko Ishizuya-Oka, Shuichi Ueda2000 年 33 巻 3 号 p. 177-184
発行日: 2000年
公開日: 2001/03/31
ジャーナル フリーDuring amphibian metamorphosis, the larval epithelium undergoes apoptosis, whereas the adult epithelium that originates from a small number of stem cells actively proliferates and differentiates into the intestinal absorptive epithelium similar to the mammalian counterpart. The larval−to−adult epithelial transformation can be triggered by a single hormone, thyroid hormone(TH), in organ cultures in vitro as well as in vivo. Thus, for the study of the organic remodeling, the amphibian intestine serves as a unique model system, where the molecular mechanisms underlying the intestinal remodeling can be clarified through the analysis of TH response genes. Recently, in the Xenopus laevis intestine, a large number of TH response genes have been cloned and become available. To assess possible functions of the TH response genes, we examined by in situ hybridization and immunohistochemical analyses the relationships between their expression pattern and the morphological changes at the cellular level. In this article, we review these recent findings that have thrown light on the molecular pathways of the intestinal remodeling, with particular focus on TH response genes involved in the epithelial−connective tissue interactions, which we previously demonstrated to be essential for the progression of the epithelial transformation.抄録全体を表示PDF形式でダウンロード (1002K) -
Yoichi Tani2000 年 33 巻 3 号 p. 185-188
発行日: 2000年
公開日: 2001/03/31
ジャーナル フリーAdvanced biotechnologies have provided benefits to detect a variety of cellular molecules for tumor diagnosis, determination of malignancy, and prognosis of cancers. Improved understanding of the molecular basis of cancers will lead to molecular targeted approaches to cancer prevention and treatment. Monoclonal antibody−based therapeutics targeting cell surface receptor such as HER2/neu/c−erbB2 oncoprotein on breast cancer cells has shown efficacy in clinical trials. HER2 pro−oncogene is a member of the epidermal growth factor receptor family, and HER2 oncoprotein is a well−characterized predictor of tumor aggressiveness. The overexpression of HER2 has been found in about 25% to 30% of breast cancers and associated with poor prognosis, resistance to hormonal therapy and lack of sensitivity to some adjuvant chemotherapy. The recombinant anti−HER2 monoclonal antibody, trastsuzumab(Herceptin), was evaluated in clinical trials for treatment of HER2−over−expressing metastatic breast cancers. The administration of the trastuzumab has produced an objective response in patients with metastatic breast cancers, suggesting that the trastuzumab will be a new molecular targeting therapeutic agent for patients with HER2−overexpressing cancer. Thus, a test result of HER2 overexpression will be a valid criterion in determining eligibility for the trastsuzumab therapy, and reliable detection of HER2 overexpression is an important prerequisite for the success of the trastsuzumab therapy.抄録全体を表示PDF形式でダウンロード (779K) -
Naomi Sakashita, Akira Miyazaki, Motohiro Takeya, Seikoh Horiuchi, Cat ...2000 年 33 巻 3 号 p. 189-194
発行日: 2000年
公開日: 2001/03/31
ジャーナル フリーAcyl coenzyme A:cholesterol acyltransferase(ACAT) is a critical enzyme participating in both intracellular cholesterol homeostasis and systemic sterol metabolism and are classified into two ACAT−isozymes termed ACAT−1 and ACAT−2. In humans, ACAT−1 is expressed ubiquitously in a variety of cells such as macrophages, enterocytes/hepatocytes, and steroid hormone−producing cells. In macrophages, immunoelectron microscopic examination revealed that ACAT−1 is localized in the endoplasmic reticulum(ER). When the cells are exposed to modified low density lipoprotein to induce foam cell transformation, a portion of ACAT−1 is shifted from ER to ER−derived small vesicles. The histological distribution of ACAT−2 in humans is still unclear, but recent data demonstrated that this isozyme is expressed in enterocytes.抄録全体を表示PDF形式でダウンロード (691K) -
Shenqiu Luo, Chen Luo, Cuilan Yang, Yingjie Piao, Harumichi Seguchi2000 年 33 巻 3 号 p. 195-200
発行日: 2000年
公開日: 2001/03/31
ジャーナル フリーIn this study, we have determined the fluorescence value of intracellular free calcium([Ca2+]i) in macrophages from mice cultured in different temperatures(37°C, 38°C and 39°C) by laser cytometer. The results indicate that hyperthermia caused elevations in the levels of [Ca2+]i. The characteristic effects of hyperthermia were, 1) rapid changes in [Ca2+]i concentration, that is to say, the fluorescence value of [Ca2+]i rose a few sec after hyperthermia; 2) a detectable relationship between the fluorescence value and temperature during defined temperature(38°C−39°C) and time(0−3600sec), 3) non−synchronous responses of cells, in other words, the extent and time of elevation of [Ca2+]i fluorescence value were different among the cells of the same group. We thought [Ca2+]i elevation induced by heat happened through the mechanism of IP3−induced calcium release(IICR) and calcium−induced calcium release(CICR). The heparin and procaine might lower the concentration of [Ca2+]i during hyperthermia through the mechanism that IICR and CICR were inhibited respectively. Free calcium influx from the extracellular medium was not thought to be necessary for heat−induced [Ca2+]i elevation.抄録全体を表示PDF形式でダウンロード (483K) -
Sergio Islas-Andrade, Ma.Cristina Revilla Monsalve, Jorge Escobedo de ...2000 年 33 巻 3 号 p. 201-208
発行日: 2000年
公開日: 2001/03/31
ジャーナル フリーDiabetic animals have contributed to the understanding of the causes, consequences and treatment of diabetes. There are different ways to induce experimental diabetes, chemically induced experimental diabetes has been widely used. Alloxan and streptozotocin are the chemicals most used. A comparative study of these two agents was performed in order to determine their effectiveness. Twenty−seven Sprague−Dawley male adult rats were intraperitoneally(i.p.) injected with 120mg/kg BW of alloxan(AL) and 27 with 60mg/kg BW of streptozotocin(STZ). Levels of glucose, insulin, triglycerides and total cholesterol were determined at different times after the i.p. injection. The effect of the two chemicals on the Langerhans’ islets was histochemically demonstrated. The reversibility of the diabetic state, 20 days after the i.p. injection of AL, was demonstrated by the recovery of insulin levels, reduction of the glucose and triglycerides concentration and by positive anti−insulin antibodies reaction in the pancreatic tissue. STZ proved to induce a more stable and permanent diabetic state.抄録全体を表示PDF形式でダウンロード (1212K) -
Kaneyuki Kubushiro, Yuichi Sakuma, Hiroshi Yamashita, Takeshi Fukuchi, ...2000 年 33 巻 3 号 p. 209-213
発行日: 2000年
公開日: 2001/03/31
ジャーナル フリーThe expression level of Lewisb(Leb) antigen was previously shown to correlate with the five−year survival rate of human endometrial cancer patients. Variant cell lines with high(SNG−S) and low(SNG−W) levels of cell surface Leb antigen were isolated from the heterogeneous endometrial cancer cell line SNG-II cells to characterize the biological significance of Leb antigen on endometrial cancer cells. SNG−S cells mainly expressed Leb, whereas SNG−W cells mainly expressed H type 1 carbohydrate antigen alternatively. In an in vitro system, SNG−W cells showed a greater capacity to adhere to cytokine−activated human umbilical vein endothelial cells. Their adhesion to human umbilical vein endothelial cells was partially inhibited by pretreatment of antibody specific for H type 1 carbohydrate antigen. These results suggested that the H type 1 carbohydrate antigen expressed on endometrial cancer cells is involved in the adhesion to endothelial cells.抄録全体を表示PDF形式でダウンロード (340K) -
Masahiro Kido, Masahide Asano, Yoichiro Iwakura, Masao Ichinose, Kazum ...2000 年 33 巻 3 号 p. 215-221
発行日: 2000年
公開日: 2001/03/31
ジャーナル フリーHistochemical analyses of β−1, 4−galactosyl−transferase(β−1, 4−GalT) I−knockout mouse liver using anti−bovine β−1, 4−GalT I antibody and Ricinus communis agglutinin(RCA)−I which interacts with oligosaccharides terminated with β−1, 4−linked galactose revealed that weak fluorescence derived from the lectin is present in the mutant mouse in contrast to the disappearance of the immunofluorescence. Lectin blot analysis of membrane glycoproteins showed that many protein bands react with RCA−I although their reactivities are weak in the mutant mouse liver. Upon pretreatment of blots with β−1, 4−galactosidase or N−glycanase, no lectin binding was observed, indicating that a part of N−linked oligosaccharides of β−1, 4−GalT I-knockout mouse liver is galactosylated by yet unidentified β−1, 4−GalT. Gel filtration column chromatography of solubilized mouse liver transferases revealed that β−1, 4−GalT activities in wild-type mouse are detected in fractions 42 and 50 while those in the mutant mouse are detected only in fraction 42. The results indicate that mouse liver contains a high molecular weight β−1, 4−GalT(110K) in addition to a previously known β−1, 4−GalT(46K).抄録全体を表示PDF形式でダウンロード (543K) -
Masanori Murakoshi, Masashi Tagawa, Rie Ikeda, Takaharu Nakayama, Kazu ...2000 年 33 巻 3 号 p. 223-229
発行日: 2000年
公開日: 2001/03/31
ジャーナル フリーThe effect of a synthetic steroidal anti−androgen, chlormadinone acetate(CMA), on spontaneous benign prostatic hyperplasia(BPH)in dogs was investigated. Male beagle dogs(5−8 years old) were divided into four experimental groups. Group 1 consisted of untreated controls. Groups 2 to 4 received CMA 0.03, 0.1, and 0.3mg/kg/day, p.o., respectively, for 6 months. In group 1, glandular hyperplasia of the prostate was clearly detected. The glandular epithelial cells showed uniformly intense nuclear staining for androgen receptor(AR). AR was also localized in the nuclei of the fibro−muscular stromal cells. Immunolocalization of 5 alpha−reductase type II was clearly detected in the interacinar stromal cells, but not in the glandular epithelial cells. In groups 2 to 4, CMA produced marked atrophy of the glandular epithelium. The interacinar fibro−muscular stroma was prominent. The intensity of the nuclear staining for AR in both epithelial and stromal cells was negative or very weak. Furthermore, the immunoreaction for 5 alpha−reductase type II in the interacinar stromal cells was negative or very weak. The decreased AR−immunostaining may be explained by the decrease in the number of AR and it may play a key role in the down−regulation of 5 alpha−reductase type II gene expression by CMA. The atrophy after treatment with CMA may be due to shrinkage of both glandular and stromal compartments in the prostate tissue.抄録全体を表示PDF形式でダウンロード (1053K)
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