巻号一覧
21 巻 (1988)
- 6 号 p. 521-
- 5 号 p. 439-
- 4 号 p. 321-
- 3 号 p. 215-
- 2 号 p. 91-
- 1 号 p. 1-
21 巻, 4 号
選択された号の論文の10件中1～10を表示しています
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YU XIU SHI, NOBUKAZU ARAKI, TOYOSHI FUJIMOTO, KAZUO OGAWA1988 年 21 巻 4 号 p. 321-326
発行日: 1988年
公開日: 2009/10/28
ジャーナル フリーAMP (2-amino-2-methyl-1-propanol) buffer was tried for the histochemical detection of alkaline phosphatase (ALPase) activity by the lead citrate method. The incubation medium tested was as follows: 170-350mM AMP, 20mM sodium β-glycerophosphate, 3.9mM magnesium sulfate, 2.0mM lead citrate and 8% sucrose; pH was adjusted to 9.2. By using this medium, ALPase activity in the liver was found in the sinusoidal and lateral surface of hepatocytes as well as in the bile canalicular surface, which was the only positive site by the original lead citrate method using Tris buffer. Moreover, the reaction product (lead phosphate) appeared finer than when using the Tris method. By quantitating ALPase activity biochemically, higher activity was found with AMP than with Tris. Apparently, this new incubation medium with AMP is suitable for detecting weak ALPase activity.抄録全体を表示PDF形式でダウンロード (5311K) -
AN IMMUNOHISTOCHEMICAL AND IN SITU HYBRIDIZATION STUDYSHIN-ICHI IZUMI, TETSUYA MORIUCHI, TAKEHIKO KOJI, MASASHI TANNO, KAZUO ...1988 年 21 巻 4 号 p. 327-342
発行日: 1988年
公開日: 2009/10/28
ジャーナル フリーOur preliminary immunohistochemical examination revealed that c-myc protein was localized in the cytoplasm of both normal and neoplastic cells. This finding did not correlate with the reported nuclear localization of c-myc protein by other analyses. To clarify this discrepancy, we investigated whether the cytoplasmic localization of c-myc protein in our preliminary immunohistochemical studies reflects true physiological habitat of the protein or the results of an artifact which occurred during tissue processing. For the immunohistochemical localization of c-myc protein, rabbit antisera against synthetic peptides which correspond to a portion of c-myc were utilized. Fixation conditions were examined by varying the temperature, the salt concentration, as well as the duration of fixation. With conventional fixation conditions used for the routine immunohistochemical localization of proteins, such as fixation with a solution of 4% formaldehyde with low salt concentration at low temperature, the c-myc protein was found in the cytoplasm of HL-60 cells, some gastric carcinoma cells, intestinal crypt epithelial cells and spermatogonia in seminiferous tubules from human and regenerating hepatocytes from rat. Whereas, when these cells and tissues were fixed at 42°C with a fixative containing 4% formaldehyde and a high concentration of NaCl, the c-myc protein was found in the nuclei of these cells. These results suggest that during fixation the c-myc protein translocates from nucleus to cytoplasm. To prove that these c-myc proteins were the product of the cells, in situ hybridization of c-myc mRNA was carried out using dinitrophenyl labeled c-myc cDNA. The gastric carcinoma cells which contained c-myc protein in their nuclei were morphologically similar to those contained the c-myc mRNA. The co-existance of c-myc protein and c-myc mRNA suggests that the c-myc protein found in the nuclei was the product of the cell.抄録全体を表示PDF形式でダウンロード (10376K) -
TORU NODA, KAZUO OGAWA1988 年 21 巻 4 号 p. 343-364
発行日: 1988年
公開日: 2009/10/28
ジャーナル フリーUsing cytochemical techniques, structural details of the Golgi apparatus were investigated under conventional and high voltage electron microscopes. Mouse duodenal absorptive epithelial cells in the crypt-base show cellular maturation during cell migration toward the tip of villus. The Golgi apparatus and its related structures also manifest several remarkable differences between immature cells located in lower part of the crypt and the mature cells in lower villus or midvillus. In immature cells in the crypt, the Golgi apparatus was well organized; simple stacking of cisternae and sparsely distributed rough ER often show face-to-face attachments not only to the trans-most cisterna of the Golgi stack, but also to the cis-most. In mature cells located at villus-base or mid-villus, the Golgi stack shows considerable development: a wavy folding to form a large highly-complex network. In the trans-side, at least three kinds of structures can be distinguished: lamellar cisterna, large-sized lipid granules (0.3-1.0μm in diameter) and trans-tubular network. The lamellar cisterna shows extraordinary independence from other Golgi stack, and it tends to detach or “peel off” from the Golgi stack. These three structures are related by structural transitions. The latter two components appeared to derive from the lamellar cisterna. In the cis-side, besides the meshed cisterna being characteristic to the cis-most cisterna, small-sized lipid granules (50nm in diameter) were often observed in the cis-most cisterna. Furthermore, cis-most cisternae have direct connections to tubular structures which extended from the apical cytoplasm. This structure is different from the trans-tubular network and contain small lipid granules identical to those observed in cis-most cisterna. From these observations, we postulate that substantial transport through Golgi apparatus might be promoted by cisterna progression from cis to trans side.抄録全体を表示PDF形式でダウンロード (32389K) -
A. TOLEDANO, M. A. BARCA, I. MORADILLO, L. RIVAS1988 年 21 巻 4 号 p. 365-381
発行日: 1988年
公開日: 2009/10/28
ジャーナル フリーThe mitochondrial metabolism of pyruvate has been studied in the cerebellar cortex at an ultrastructural level in rats 3 to 36 months old using the technique of the accumulation of Sr2+ (15, 17). Shape, size and number of the reaction products were the basis to distinguish the types of mitochondria related to the distinct activity levels of the enzyme pyruvate dehydrogenase. Important differences were detected between: 1) different types of neuronal and glial cells; 2) different neuronal parts (soma, dendrites and axons); and 3) neurons and filial cells of young (3 to 6 months old) and senile animals (24 to 36 months old). Animals 3 to 18 months old presented a constant activity pattern for each cell type and for the different cytoplasmatic regions studied. Purkinje cells, in animals of this age group, are the most active and display a high intensity in their soma with a progressive diminution towards the endings of the dendrites. Granule cells present an intermediate intensity in soma and dendrites. Remaining neurons exhibit low somatic intensities. Axons of short axon cells were usually negative, but afferent fibers were positive. Mossy fibers display various types of reaction products.
Animals 24 to 36 months old maintain this reaction pattern, but the mitochondrial reaction pattern, but the mitochondrial reaction is heterogenous. Purkinje and granule cells exhibit the greatest amount of diminution. Some cells and cellular regions (granule dendrites and astrocytes) may show a stronger reaction than in young animals. Results are related to the pre-or post-synaptic location of the reaction product, mitochondrial and cellular involution, and reactive and plastic phenomena of aging.抄録全体を表示PDF形式でダウンロード (13943K) -
GIULIO MARINOZZI, FABIO FERRANTE, EUGENIO GAUDIO, PIERANGELO GEPPETTI, ...1988 年 21 巻 4 号 p. 383-387
発行日: 1988年
公開日: 2009/10/28
ジャーナル フリーNoradrenaline levels and distribution in different components of rat knee joint were studied using radioenzymatic assay and glyoxylic acid histofluorescence techniques.
The articular capsule possessed the highest noradrenaline content, and the levels of noradrenaline in cruciate ligaments were slightly higher than in collateral ligaments. In all joint components, noradrenergic nerve fibres were associated almost exclusively with the blood vessels.
The possible involvement of a noradrenergic action on joint microcirculation in the pathophysiology of rheumatoid arthritis is discussed.抄録全体を表示PDF形式でダウンロード (1851K) -
RYOICHI SHIMONO, MASAHISA SHIMADA1988 年 21 巻 4 号 p. 389-400
発行日: 1988年
公開日: 2009/10/28
ジャーナル フリーTissue distribution and metabolic fate of 35S from 35S-taurine in pregnant mice were studied by whole-body autoradiography and biochemical analysis.
Whole-body autoradiography was performed at 30min, 3 and 6hr after intravenous injection of 35S-taurine. In the maternal body, high densities were observed in the kidney, liver, bile and digestive tract, while low densities were in the blood and brain. The placenta showed a relatively high density, but the fetus was low density.
As for the biochemical analysis, the mice were decapitated at the same intervals after injection as those for autoradiography and various tissues were removed. These tissues were fractionated into 6% perchloric acid-soluble, -insoluble and lipid fractions. Total radioactivities (cpm) per wet weight (g) were high in the liver, small intestine and kidney and low in the brain, blood and fetus. These data were consistent with those of whole-body autoradiographs. The greatest part (over 98%) of total radioactivities in all tissues was incorporated into the acid-soluble fraction.
The acid-soluble fraction of each tissue was analyzed by thin-layer chromatography. The radioactive spots were examined for taurine and taurocholic acid in the bile, but only for taurine in all other tissues.抄録全体を表示PDF形式でダウンロード (3935K) -
MORIMASA MATSUTA, TERUO KAGABU, IWAO NISHIYA1988 年 21 巻 4 号 p. 401-411
発行日: 1988年
公開日: 2009/10/28
ジャーナル フリーFour cases of ovarian cancer (mucinous cystadenocarcinoma, serous cystadenocarcinoma, endometrioid carcinoma and metastatic cancer of the colon) which revealed diffuse localization of carbohydrate antigen 19-9 (CA 19-9) in their tumor tissues were subjected to immunoelectron microscopy. In all cases CA 19-9 was present in plasma membranes, microvilli and glycocalyx in mucinous and metastatic carcinoma, indicating that the antigenic determinant for CA 19-9 is sialylated Lewis A. Reaction products were seldom seen in perinuclear spaces and endoplasmic reticula. Many pictures revealed positive vesicles apparently released from the Golgi apparatus; this feature seemed to be characteristic of the findings. Thus, the Golgi apparatus is considered to be the place where immunoreactive CA 19-9 is first produced in cancer cells. Since positive images were found not only in organelle but also on collagen fibers directly under the basement membranes, it seemed that an important factor for CA 19-9 increase in the blood is the expansion of CA 19-9 to stroma.
It was strongly suggested that CA 19-9 was useful as a tumor marker for ovarian cancer which originated in common epithelial cells.抄録全体を表示PDF形式でダウンロード (14616K) -
IV. IMMUNOCYTOCHEMICAL INVESTIGATIONSGERD POEGGEL, HANS LUPPA, ANDREA SCHMIDT1988 年 21 巻 4 号 p. 413-421
発行日: 1988年
公開日: 2009/10/28
ジャーナル フリーCyclic nucleotide phosphodiesterase (PDE) was demonstrated by an immunocytochemical method on the electron microscopic level. The method was chosen was a preembedding PAP-technique with osmification of the diamino benzidine (DAB) reaction product. Antigenic sites of PDE were detected in synapses, on endoplasmic reticulum, microtubules, the nuclear envelope, the cytoplasma and processes of distinct neurons and glial cells. Only a little reaction product was found in the capillary endothelium. The morphology was better in comparison to enzymehistochemical methods. There was a good agreement between the localizations of the enzymatic reaction (16) and the antigenic sites of the PDE on the electron microscopic level.抄録全体を表示PDF形式でダウンロード (13111K) -
TORU NODA, GUAN-JUN LI, KAZUO OGAWA1988 年 21 巻 4 号 p. 423-432
発行日: 1988年
公開日: 2009/10/28
ジャーナル フリーPDF形式でダウンロード (9452K) -
YU XIU SHI, TOYOSHI FUJIMOTO, KAZUO OGAWA1988 年 21 巻 4 号 p. 433-435
発行日: 1988年
公開日: 2009/10/28
ジャーナル フリーPDF形式でダウンロード (2839K)
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