Bioscience, Biotechnology, and Biochemistry
Online ISSN : 1347-6947
Print ISSN : 0916-8451
Volume 69 , Issue 6
Showing 1-24 articles out of 24 articles from the selected issue
Organic Chemistry Note
Biochemistry & Molecular Biology Regular Papers
  • Takahiro KANEKO, Toshihisa OHNO, Naganori OHISA
    2005 Volume 69 Issue 6 Pages 1073-1081
    Published: 2005
    Released: June 23, 2005
    JOURNALS FREE ACCESS
    A raw starch utilizing microbe was isolated from mud in a milling factory. The 16S ribosomal DNA (rDNA) sequencing and morphological properties of the strain indicated that it belongs to the genus Streptomyces. A strongly raw starch digesting amylase was purified from the culture supernatant of the strain by chromatographic procedures. The specific activity of the enzyme was 11.7 U/mg, molecular mass 47 kDa, optimum pH 6.0, and optimum temperature 50 to 60 °C. The enzyme showed sufficient activity even at 70 °C. It was activated by calcium, cobaltous, and magnesium ions, and inhibited by copper, nickel, zinc, and ferrous ions. It formed maltose mainly from raw and gelatinized starch, and glycogen. No products were formed from glucose, maltose, maltotriose, pullulan, or cyclodextrins (CDs). The enzyme digested raw wheat, rice, and waxy rice starch rapidly, and raw corn, waxy corn, sweet potato, tapioca, and potato starch normally.
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  • Hisashi HOSHIDA, Tsuneyasu FUJITA, Kenji MURATA, Kanako KUBO, Rinji AK ...
    2005 Volume 69 Issue 6 Pages 1090-1097
    Published: 2005
    Released: June 23, 2005
    JOURNALS FREE ACCESS
    Laccase is a multicopper-containing enzyme that catalyzes the oxidation of phenolic compounds. lcc1 cDNA coding for a secretory laccase of Pycnoporus coccineus was expressed under the maltose inducible amyB promoter in Aspergillus oryzae and under the galactose inducible GAL10 promoter in Saccharomyces cerevisiae. Laccase activities, which were undetectable in the absence of copper, were observed by increasing copper concentrations in the media for both systems. The amounts of secreted laccase protein but not lcc1 mRNA increased in proportion to copper concentrations in A. oryzae. The extracellular activities of native A. oryzae amylase and recombinant RNase-T1 expressed from the same amyB promoter in A. oryzae were constant regardless of copper concentrations. Our results indicate that a high copper concentration is required for the production of active laccase in heterologous hosts and that the copper is required for a post-transcriptional process.
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  • Änne GLASS, Jeannette HENNING, Thomas KAROPKA, Thomas SCHEEL, Sve ...
    2005 Volume 69 Issue 6 Pages 1098-1103
    Published: 2005
    Released: June 23, 2005
    JOURNALS FREE ACCESS
    Designing microarray experiments, scientists are often confronted with the question of pooling due to financial constraints, but discussion of the validity of pooling tends toward a sub-pooling recommendation. Since complete pooling protocols can be considered part of sub-pooling designs, gene expression data from three complete pooling experiments were analyzed. Data from complete pooled versus individual mRNA samples of rat brain tissue were compared to answer the question whether the pooled sample represents individual samples in small-sized experiments. Our analytic approach provided clear results concerning the Affymetrix® MAS 5.0 signal and detection call parameters. Despite a strong similarity of arrays within experimental groups, the individual signals were evidently not appropriately represented in the pooled sample, with slightly more than half of all the genes considered. Our analysis reveals problems in cases of small complete pooling designs with less than six subjects pooled.
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  • Kwan Kit WOO, Yoshinobu KIMURA
    2005 Volume 69 Issue 6 Pages 1111-1119
    Published: 2005
    Released: June 23, 2005
    JOURNALS FREE ACCESS
    In our previous study (Woo, K. K., et al., Biosci. Biotechnol. Biochem., 68, 2547–2556 (2004), we purified an α-mannosidase from Ginkgo biloba seeds; it was activated by cobalt ions and highly active towards high-mannose type free N-glycans occurring in plant cells. In the present study, we have found that the substrate specificity of Ginkgo α-mannosidase is significantly regulated by cobalt ions. When pyridylamino derivative of Man9GlcNAc2 (M9A) was incubated with Ginkgo α-mannosidase in the absence of cobalt ions, Man5GlcNAc2-PA (M5A) having no α1–2 mannosyl residue was obtained as a major product. On the other hand, when Man9GlcNAc2-PA was incubated with α-mannosidase in the presence of Co2+ (1 mM), Man3–1GlcNAc2-PA were obtained as major products releasing α1–3/6 mannosyl residues in addition to α1–2 mannosyl residues. The structures of the products (Man8–5GlcNAc2-PA) derived from M9A by enzyme digestion in the absence of cobalt ions were the same as those in the presence of cobalt ions. These results clearly suggest that the trimming pathway from M9A to M5A is not affected by the addition of cobalt ions, but that hydrolytic activity towards α1–3/6 mannosyl linkages is stimulated by Co2+. Structural analysis of the products also showed clearly that Ginkgo α-mannosidase can produce truncated high-mannose type N-glycans, found in developing or growing plant cells, suggesting that α-mannosidase might be involved in the degradation of high-mannose type free N-glycans.
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  • Hirohide TOYAMA, Wichai SOEMPHOL, Duangtip MOONMANGMEE, Osao ADACHI, K ...
    2005 Volume 69 Issue 6 Pages 1120-1129
    Published: 2005
    Released: June 23, 2005
    JOURNALS FREE ACCESS
    There are two types of membrane-bound D-sorbitol dehydrogenase (SLDH) reported: PQQ–SLDH, having pyrroloquinoline quinone (PQQ), and FAD–SLDH, containing FAD and heme c as the prosthetic groups. FAD–SLDH was purified and characterized from the PQQ–SLDH mutant strain of a thermotolerant Gluconobacter frateurii, having molecular mass of 61.5 kDa, 52 kDa, and 22 kDa. The enzyme properties were quite similar to those of the enzyme from mesophilic G. oxydans IFO 3254. This enzyme was shown to be inducible by D-sorbitol, but not PQQ–SLDH. The oxidation product of FAD–SLDH from D-sorbitol was identified as L-sorbose. The cloned gene of FAD–SLDH had three open reading frames (sldSLC) corresponding to the small, the large, and cytochrome c subunits of FAD–SLDH respectively. The deduced amino acid sequences showed high identity to those from G. oxydans IFO 3254: SldL showed to other FAD-enzymes, and SldC having three heme c binding motives to cytochrome c subunits of other membrane-bound dehydrogenases.
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  • Masayuki KOBAYASHI, Yoichiro FUJIOKA, Takayuki MORI, Minoru TERASHIMA, ...
    2005 Volume 69 Issue 6 Pages 1130-1136
    Published: 2005
    Released: June 23, 2005
    JOURNALS FREE ACCESS
    Photosynthetic reaction centers (RCs) and their core light-harvesting complexes (LH1-RCs), purified from a thermophile, Thermochromatium (T.) tepidum, and a mesophile, Allochromatium (A.) vinosum, were reconstituted into liposomes. The RC and the LH1-RC in the reconstituted liposomes were found intact from the absorption spectra at about 4 and 40 °C respectively. The thermal stability of the RCs of T. tepidum in the liposome was dependent on whether they were surrounded directly by lipids or by the core light-harvesting complexes. The results show that the RC of T. tepidum gains its thermostability through interactions with the LH1. These results are consistent with the result that the thermal stability of the LH1 in T. tepidum is similar in both the reconstituted LH1-RC liposome and ICM. This is clearly different from the mesophilic bacterium, A. vinosum. The thermal stability of RC was also affected by its subunit constitution: the RC containing a cytochrome subunit was more thermostable than the cytochrome-detached RC. This suggests that the cytochrome subunit might play a role in protecting the special pair pigments from denaturation. The thermal denaturation showed a second-order reaction dependence on time. The interaction of the pigments with proteins and/or lipids might be the cause of the second-order reaction profile.
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  • Yoshihiko ONDA, Kikukatsu ITO
    2005 Volume 69 Issue 6 Pages 1156-1161
    Published: 2005
    Released: June 23, 2005
    JOURNALS FREE ACCESS
    The spadix of skunk cabbage, Symplocarpus foetidus, is thermogenic and maintains an internal temperature of around 20 °C even when the ambient air temperature drops below freezing. This homeothermic heat production is observed only during the stigma stage, and thereafter ceases at the male stage when pollen is shed. To clarify the regulatory mechanism by which the stigma stage-specific heat production occurs in the spadix, sugars, organic acids, and amino acids in xylem sap were analyzed and compared with those of post-thermogenic plants. Interestingly, no significant difference was observed in the total volume of xylem sap per fresh weight of the spadix between thermogenic (31.2±24.7 μl h−1g−1) and post-thermogenic (50.5±30.4 μl h−1g−1) plants. However, concentrations of sugars (sucrose, glucose, and fructose), organic acids (malate and succinate), and amino acids (Asp, Asn, Glu, Gln, Gly, and Ala) in xylem sap decreased remarkably in post-thermogenic plants. Our results indicate that the composition of the xylem sap differs during the development of the spadix of S. foetidus.
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  • Fumihiko OMURA, Atsushi FUJITA, Keiji MIYAJIMA, Nobuyuki FUKUI
    2005 Volume 69 Issue 6 Pages 1162-1171
    Published: 2005
    Released: June 23, 2005
    JOURNALS FREE ACCESS
    The Saccharomyces cerevisiae Put4 permease is significant for the transport of proline, alanine, and glycine. Put4p downregulation is counteracted by npi1 mutation that affects the cellular ubiquitination function. Here we describe mutant Put4 permeases, in which up to nine lysine residues in the cytoplasmic N-terminal domain have been replaced by arginine. The steady-state protein level of the mutant permease Put4-20p (Lys9, Lys34, Lys35, Lys60, Lys68, Lys71, Lys93, Lys105, Lys107 → Arg) was largely higher compared to that of the wild-type Put4p, indicating that the N-terminal lysines can undergo ubiquitination and the subsequent degradation steps. Proline is the only amino acid that yeast assimilates with difficulty under standard brewing conditions. A lager yeast strain provided with Put4-20p was able to assimilate proline efficiently during beer fermentations. These results suggest possible industrial applications of the mutant Put4 permeases in improved fermentation systems for beer and other alcoholic beverages based on proline-rich fermentable sources.
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  • Takeshi IKEDA, Masahiro YAMAMOTO, Hiroyuki ARAI, Daijiro OHMORI, Masah ...
    2005 Volume 69 Issue 6 Pages 1172-1177
    Published: 2005
    Released: June 23, 2005
    JOURNALS FREE ACCESS
    A thermophilic, obligately chemolithoautotrophic hydrogen-oxidizing bacterium, Hydrogenobacter thermophilus TK-6, assimilates carbon dioxide via the reductive tricarboxylic acid cycle. A gene encoding a ferredoxin involved in this cycle as an electron donor (HtFd1) was cloned and sequenced. Interestingly, another ferredoxin gene (encoding HtFd2) was found in tandem with the HtFd1 gene. These two ferredoxin genes overlapped by four bp, and transcriptional analysis revealed that they are co-transcribed as an operon. The deduced amino acid sequences of HtFd1 and HtFd2 were 42.9% identical and each contained four cysteine residues that serve as probable ligands to an iron-sulfur cluster. Spectroscopic analyses of the purified recombinant ferredoxins heterologously expressed in Escherichia coli indicated that each ferredoxin contains a single [4Fe–4S]2+⁄1+ cluster.
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  • Minoru UJITA, Yosuke KATSUNO, Ichiro KAWACHI, Yoshinori UENO, Yutaka B ...
    2005 Volume 69 Issue 6 Pages 1178-1185
    Published: 2005
    Released: June 23, 2005
    JOURNALS FREE ACCESS
    The silkworm Bombyx mori 30-kDa lipoproteins (6G1 and 19G1), major components of the hemolymph, were shown to bind to glucans. 6G1 apolipoprotein was expressed as a fusion protein with glutathione S-transferase in Escherichia coli and assayed for its binding activity. The purified recombinant 6G1 apolipoprotein specifically bound to β-glucan, but not to chitin, mannan, peptidoglycan, or oligosaccharide chains on glycoproteins. The β-glucan binding of the recombinant 6G1 was inhibited by laminaribiose and laminarin, a soluble glucan, but not by lipopolysaccharide or insect blood sugar, trehalose at physiological concentration. Furthermore, the recombinant 6G1 was shown to participate in the activation of prophenoloxidase cascade and to interfere with hyphal growth of the entomopathogenic fungus Paecilomyces tenuipes, injected into pupae of B. mori. These results suggest that 6G1 lipoprotein plays a role in the protection of B. mori against invading fungi.
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Biochemistry & Molecular Biology Notes
Food & Nutrition Science Regular Papers
  • Meiyu XU, Yoshimasa SUGIURA, Satoshi NAGAOKA, Yoshihiro KANAMARU
    2005 Volume 69 Issue 6 Pages 1082-1089
    Published: 2005
    Released: June 23, 2005
    JOURNALS FREE ACCESS
    Potent inhibition of cell proliferation was found for commercial preparations of bovine α-lactalbumin on cultured intestinal cell line IEC-6 albeit lot-dependent. The inhibition was irreversible and a single exposure to the culture medium containing α-lactalbumin of an active lot for a period as short as 30 min was enough to provoke cell death, possibly through apoptosis. The oligomer fraction from size exclusion chromatography was significantly robust, while the monomer fraction remained totally inert, in inducing cell death. Incubation at 37 °C for 5 d with 30% trifluoroethanol in acetate, pH 5.5, in a slowly rotating test tube rendered the monomer fraction cytotoxic. Again, the resulting inhibitory activity was found in the oligomer fraction from size exclusion chromatography, with emergence of subtle peaks at 22- and 30-kDa. Furthermore, the occurrence of SDS-stable 30-kDa as well as 20-kDa bands on electrophoresis was a common feature for α-lactalbumin with the activity inducing cell death. Thus, a certain dimeric state can be implicated in the cytotoxicity of bovine α-lactalbumin.
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  • Kimiko KIMURA, Kazuo KOBAYASHI, Hiroyuki NAITO, Yoshihiko SUZUKI, Yosh ...
    2005 Volume 69 Issue 6 Pages 1104-1110
    Published: 2005
    Released: June 23, 2005
    JOURNALS FREE ACCESS
    We examined the effect of lactational exposure to tributyltin on innate immunodefenses in the F1 generation using in vivo and in vitro experiments. Pregnant C57BL/6 mice were given drinking water containing 0, 15, or 50 μg/ml of tributyltin chloride (TBTCl) from parturition to weaning. At weaning time, offspring were inoculated with Escherichia coli K-12, and bacterial clearances from the peritoneal cavity and spleen were examined. In vivo infection experiments indicated that bacterial clearance was significantly depressed in offspring breast-fed by dams exposed to 15 μg/ml of TBTCl (15 ppm F1), but not in offspring by dams exposed to 50 μg/ml of TBTCl (50 ppm F1). In vitro functional assays revealed that the killing activity of neutrophils decreased significantly in 15 ppm F1, but not in 50 ppm F1. We suggest that lactational exposure to TBT impairs innate immunodefenses in the F1 generation against non-pathogenic bacterial infection.
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  • Masako ITO, Takeo KATO, Tsukasa MATSUDA
    2005 Volume 69 Issue 6 Pages 1137-1144
    Published: 2005
    Released: June 23, 2005
    JOURNALS FREE ACCESS
    The rice grains (RG) and rice seed proteins remaining in rice miso were investigated with a view point to the potential allergenicity of rice miso. RG ranging from 36 to 180 mg dry weight per g dry miso were separated from several samples of commercially available rice miso. Scanning electron microscopy of the recovered RG indicated that starch granules disappeared almost completely while protein bodies remained intact in RG. Most of the major seed proteins were extracted from RG by heating with 1% SDS/2% 2-mercaptoethanol and detected by SDS–polyacrylamide gel electrophoresis. Major rice allergenic proteins, 14–16 kDa albumin (Alb14–16) and α-globulin (α-Glb) were also detected by immunoblotting using the specific antisera, and their contents were estimated to be 1.7 to 9.0 and 1 to 7 mg protein per g dry RG respectively. However, the major rice proteins, including glutelin and prolamin, in RG were insoluble in salt, alcohol, and urea solutions, but soluble in 6 M guanidine hydrochloride (Gu–HCl). By immunoblotting and ELISA, no Alb14–16 and only a slight amount of α-Glb were detected even in the 6 M Gu–HCl fraction, indicating that these major allergenic proteins are denatured and are present in an insoluble form in rice miso.
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Food & Nutrition Science Notes
  • Toshiaki HANDA, Kohji YAMAGUCHI, Yoshikatsu SONO, Kazunaga YAZAWA
    2005 Volume 69 Issue 6 Pages 1186-1188
    Published: 2005
    Released: June 23, 2005
    JOURNALS FREE ACCESS
    It was found that fenugreek seed extract reduced the body weight gain induced by a high-fat diet in obese mice. The extract decreased plasma triglyceride gain induced by oil administration. The major component of the extract, 4-hydroxyisoleucine, also decreased plasma triglyceride gain. Consequently, fenugreek seed extract is expected to prevent the obesity induced by a high-fat diet.
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  • Meiyu XU, Yoshimasa SUGIURA, Satoshi NAGAOKA, Yoshihiro KANAMARU
    2005 Volume 69 Issue 6 Pages 1189-1192
    Published: 2005
    Released: June 23, 2005
    JOURNALS FREE ACCESS
    Monomeric 14-kDa bovine α-lactalbumin was purified with a preparation of lower molecular weight whey protein concentrate from Holstein cow normal milk followed by size exclusion chromatography. The protein showed a stimulatory rather than an inhibitory effect on the proliferation of a cultured IEC-6 cell line from the rat small intestine. But incubation in 30% trifluoroethanol/acetate buffer (pH 5.5) at 37 °C for 5 d in a slowly rotating test tube rendered it highly cytotoxic with concomitant appearance of SDS-stable 20- and 30-kDa forms of α-lactalbumin on electrophoresis. Furthermore, α-lactalbumin obtained by a one-step purification procedure by affinity chromatography on an anti-α-lactalbumin antibody column from the lower molecular weight whey protein concentrate, which had been found to contain several SDS-stable higher Mr forms of α-lactalbumin, exhibited potent inhibitory activity on IEC-6 cell growth. These results indicate the involvement of SDS-stable higher Mr forms of bovine normal milk α-lactalbumin in inducing cell death on the intestinal IEC-6 cell line.
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  • Hiroshi TERANO, Kaori TAKAHASHI, Yutaka SAKAKIBARA
    2005 Volume 69 Issue 6 Pages 1217-1220
    Published: 2005
    Released: June 23, 2005
    JOURNALS FREE ACCESS
    The germination behaviors of spores of Alicyclobacillus acidoterrestris, which has been considered to be a causative microorganism of flat sour type spoilage in acidic beverages, were investigated. The spores of A. acidoterrestris showed efficient germination and outgrowth after heat activation (80 °C, 20 min) in Potato dextrose medium (pH 4.0). Further, the spores treated with heat activation germinated in McIlvaine buffer (pH 4.0) in the presence of a germinative substance (L-alanine) and commercial fruit juices, although not in phosphate buffer (pH 7.0). Heat activation was necessary for germination. The spores of A. acidoterrestris, which easily survived the heat treatment in acidic conditions, lost their resistance to heat during germination. Our results suggest that the models obtained from spore germination of A. acidoterrestris might be beneficial to determine adequate thermal process in preventing the growth of potential spoilage bacteria in acidic beverages.
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Microbiology & Fermentation Technology Regular Papers
  • Nicole A. BLECKWENN, William E. BENTLEY, Joseph SHILOACH
    2005 Volume 69 Issue 6 Pages 1065-1072
    Published: 2005
    Released: June 23, 2005
    JOURNALS FREE ACCESS
    Adaptation of the vaccinia virus expression system to HeLa S3 suspension bioreactor culture for the production of recombinant protein was conducted. Evaluation of hollow fiber perfusion of suspension culture demonstrated its potential for increased cell density prior to infection. The hollow fiber was also used for medium manipulations prior to infection. Two process parameters, multiplicity of infection (MOI) and temperature during the protein production phase, were evaluated to determine their effect on expression of the reporter protein, enhanced green fluorescent protein (EGFP). An MOI of 1.0 was sufficient for infection and led to the highest level of intracellular EGFP expression. Reducing the temperature to 34 °C during the protein production phase increased production of the protein two-fold compared to 37 °C in spinner flask culture. Scaling up the process to a 1.5-liter bioreactor with hollow fiber perfusion led to an overall production level of 9.9 μg EGFP/106 infected cells, or 27 mg EGFP per liter.
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  • Naoya SHINZATO, Mizuho MURAMATSU, Toru MATSUI, Yoshio WATANABE
    2005 Volume 69 Issue 6 Pages 1145-1155
    Published: 2005
    Released: June 23, 2005
    JOURNALS FREE ACCESS
    The phylogenetic diversity of the bacterial community in the gut of the termite Coptotermes formosanus was investigated using a 16S rRNA gene clone library constructed by PCR. After screening by restriction fragment length polymorphism (RFLP) analysis, 49 out of 261 clones with unique RFLP patterns were sequenced and phylogenetically analyzed. Many of the clones (94%) were derived from Bacteroidales, Spirochaetes, and low G+C content gram-positive bacteria consisting of Clostridiales, Mycoplasmatales, Bacillales, and Lactobacillales. In addition, a few clones derived from Actinobacteria, Proteobacteria, Planctomycetes, Verrucomicrobia, and the candidate phylum “Synergistes” were also found. The most frequently identified RFLP type, BCf1-03, was assigned to the order Bacteroideales, and it constituted about 70% of the analyzed clones. The phylogenetic analysis revealed that the representative clones found in this study tended to form some clusters with the sequences cloned from the termite gut in several other studies, suggesting the existence of termite-specific bacterial lineages.
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Microbiology & Fermentation Technology Note
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