巻号一覧
54 巻 (1987)
- 6 号 p. 573-
- 5 号 p. 471-
- 4 号 p. 362-
- 3 号 p. 219-
- 2 号 p. 105-
- 1 号 p. 3-
54 巻, 4 号
選択された号の論文の11件中1～11を表示しています
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伊東 博信1987 年 54 巻 4 号 p. 362-372
発行日: 1987/08/15
公開日: 2009/12/04
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大石 宏1987 年 54 巻 4 号 p. 373-392
発行日: 1987/08/15
公開日: 2009/12/04
ジャーナル フリーThough many morphological studies of the kidney have been reported, there are few studies with special reference to the hilus itself. This paper describes the hilus of 50 kidneys in 25 Japanese adults.
The arteries, veins and ureter were filled with latex rubber of different colors, and the hilus was cut along the long axis. The relative size of the hilus was calculated by the ratio of the area of hilus to that of the medial surface of the kidneys. The position of the hilus was estimated by mesasuring it's height on the long axis of the medial border of the kidneys (Fig. 1). The hilus was divided into 4 areas by horizontal and vertical lines passing through the center of the sectioned ureter (Fig. 2), and the number of blood vessels was counted in each area.
The results were as follows:
1) The shape of the hilus was irregular in apporoximately 80% of the kidneys.
2) The mean area of the hilus was 14.7 (5.5-30.9)% of the medial surface area of the kidneys.
3) The hilus was situated on the center of the long axis of the medial border of the kidneys.
4) The average numbers of arteries inside and outside the hilus were 5.94 (3-11), and 1.58 (0-8), respectively.
5) The average numbers of veins inside and outside the hilus were 4.08 (2-8), and 0.12 (0-2), respectively.
6) The number of arteries in the hilus was much larger than that of veins.
7) The number of blood vessels in each area of the hilus was as follows: (1)5.40 (2-10) in the anterosuperior part, (2) 2.33 (0-6) in the anteroinferior part, (3) 1.21 (0-9) in the posterosuperior part, (4) 0.86 (0-4) in the posteroinferior part.抄録全体を表示PDF形式でダウンロード (4032K) -
岩谷 了1987 年 54 巻 4 号 p. 393-402
発行日: 1987/08/15
公開日: 2009/12/04
ジャーナル フリーBy applying the crises theory we tried to evaluate the mentality and activities of diseased elderly people with different experiences and living circumstances. The analysed data were the results of Activities of Daily Living (ADL), Hasegawa's Dementia Rating Scale (DRS), interviews with each person and the Color Choice Test (CCT).
1) The results of ADL on admission to the hospital and ADL and DRS at the time of investigation differenced depending on sex, living circumstances and economic conditions. These who had been living alone (with no family members) were often hospitalized regardless of their higher ADL.
2) There was a significant but not high correlation between ADL and DRS.
3) Compared with two groups of patients by improved and deteriorated ADL after hospitalization, the above results showed much diversity, varied in each person.
4) With the interview data and on the basis of the crises theory, we found two types of patient. One who gives up and collapses, and one who fights on and gets over. Patients of the former type have never actively overcome the difficulties in their past. Patients of the latter type overcome their difficulties, and still made good use of the experience. Besides these two types we found two inbetween types. Some patients chose only a simple world denoting their simple personality and limited activities (TYPE 1). The others persistently behaved as young day's favorable situations (TYPE 2). The patients of these in-between types, especially outpatients, may have the same prognosis as those collapse type, requiring special consideration medically.
5) CCT revealed a difference between in-and outpatients in their emotional state. Inpatients showed smaller emotional undulations. Five stimulation words added to the previous standard words showed the medical staff had a great influence on the patients' emotions.
From the results we conclude the following two points are important for the medical care of elderly patients: (1)"disease" should be recognized as a crisis, (2) patients should be helped to grow old by focusing on their strange, as well as helping strange materially from the outside. Medical staff should consider these two points stimultaneously.抄録全体を表示PDF形式でダウンロード (676K) -
山本 隆彰1987 年 54 巻 4 号 p. 403-412
発行日: 1987/08/15
公開日: 2009/12/04
ジャーナル フリーIt has been reported that group A streptococci isolated from patients with acute post-streptococcal glomerulonephritis (APSGN) produce a unique extracellular protein which has a molecular weight of 46kd as determined by sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE). This protein is designated as the nephritis-strain associated protein (NSAP), and is generally not secreted by the streptococcal strain isolated from patients not having APSGN. The present paper describes the purification and biological characterization of NSAP.
Group A streptococcus, type 12, strain A374 was cultured in a chemically defined medium (CDM) at 37°C for 18 hours and proteins in the supernatant fluid were precipitated by ammonium sulfate. This preparation was subjected to a DEAE Sepharose CL-6B column chromatography. NSAP was eluted in the presence of 0.2M NaCl and this fraction was precipitated with ammonium sulfate, and then applied to a Sephadex G-100 column. The fraction containing NSAP was precipitated with ammonium sulfate and finally applied to a chromatofocusing column. NSAP was eluted at a pI 4.78. The purity of NSAP was identified with SDS-PAGE and Western blot analysis using monoclonal antibodies against NSAP.
Purified NSAP has streptokinase activity, but NSAP possesses antigenic domains different from those present in streptokinase derived from group C streptococcus. Serum complement of guinea pig was also inactivated by NSAP and administration of NSAP caused inflammatory change in the subcutaneous region of the guinea pig.抄録全体を表示PDF形式でダウンロード (2911K) -
吉村 和泰1987 年 54 巻 4 号 p. 413-418
発行日: 1987/08/15
公開日: 2009/07/10
ジャーナル フリーGroup A streptococci isolated from patients with acute post-streptococcal glomerulonephritis (APSGN) secret a protein that has mol. wt. 46 kd. This protein is generally not secreted by streptococ-cal strain isolated from non-APSGN patients. This unique protein is designated as nephritis-strain associated protein (NSAP).
In this communication, the author has analysed the presence of NSAP in streptococcal strains isolated from patients with and without APSGN by Western blot analysis using mAb specific to NSAP.
Western blot analysis which was achieved using two different anti-NSAP mAb (both N-59-T and R-60-J) showed that NSAP has at least two antigenic epitopes. N-59-T mAb recognized the same antigenic determinant which was shared in both NSAP and group C streptokinase, and R-60-J mAb reacts with only NSAP.
Sixty two strains of group A, 4 strains of group B, 6 strains of group C and 8 strains of group G streptococci were analysed for the presence of NSAP using two monoclonal antibodies (N-59-T and R-60-J) in Western blot analysis of TCA precipitates of crude ECP. These strains were selected at random from clinical laboratory specimens. However, it was unclear whether these strains had been isolated from APSGN patients. When antibody N-59-T was used, 31 strains of 62 group A streptococ-cal strains (50%), 1 of 4 group B strains (25%), and all of the group C and G strains reacted to this antibody. When antibody R-60-J was used, 16 strains of 62 group A streptococcal strains (26%) reacted to this antibody; however, the group B, C and G strains did not react.
In the experiment detecting the presence of NSAP with streptococci isolated from well-documented cases of APSGN, all 14 group A strains reacted to antibody N-59-T. Antibody R-60-J could react to 12 strains of these 14 (86%). when three group G streptococcal strains were analysed, antibody N-59-T reacted to all strains: however, antibody R-60-T recognized 2 out of 3 strains (67%).抄録全体を表示PDF形式でダウンロード (2600K) -
ウシガエル赤血球酵素の精製と性質伊籐 敏治1987 年 54 巻 4 号 p. 419-428
発行日: 1987/08/15
公開日: 2009/07/10
ジャーナル フリーThe present paper describes the purification and characterization of NADPH-dependent methemoglobin reductase from the erythrocytes of bullfrog, Rana catesbeiana. The results obtained are as follows:
1) Two active protein components having different isoelectric points (pI) of 5.0 and 7.5 were separated and purified to homogeneity.
2) Both components had the same molecular weight of 22, 000 upon SDS-polyacrylamide gel electrophoresis. pI 5.0 component was faint yellow showing a broad absorption in the range of 400-450 nm, while pI 7.5 component was colorless showing no absorption in that range.
3) When PI 5.0 component was subjected to the second isoelectric focusing, the protein part of that component was observed to regenerate at pI 7.5, leaving the yellow color at acid pH. This fact strongly implies that the protein moiety of pI 5.0 component is identical to that of pI 7.5 component.
4) Both components catalysed the NADPH-dependent MB (or DCPIP, FMN, FAD) reduction. Kinetic studies revealed that both components were the same in their catalytic properties.
5) These structural and functional properties of the bullfrog enzyme very much resembled those of NADPH-flavin reductase in the human erythrocytes, indicating that the enzyme equivalent to that found in mammalian erythrocytes also exists in amphibian erythrocytes.抄録全体を表示PDF形式でダウンロード (1713K) -
米山 芳雄1987 年 54 巻 4 号 p. 429-440
発行日: 1987/08/15
公開日: 2009/07/10
ジャーナル フリーThe nucleotide sequences of the D-loop region, the light (L) strand replication origin and the seven tRNA genes of the Rana catesbeiana mitochondrial genome were determined and compared with their counterparts of other higher vertebrate mitochondrial genomes.
The results obtained are as follows:
1) The D-loop region consists of 2, 187 nucleotides and is flanked by the cyt b gene on the 5' end and by the genes for tRNALeu(CUN), tRNAThr, tRNAPAPro and tRNAPhe on the 3' end. These genes are distinctly different in their organization from those in other higher vertebrates.
2) The overall sequence homology between the D-loop regions of Rana catesbeiana and other higher vertebrates is rather low. The sequences known as CSB-1, 2 and 3, and TAS, however, are found to be conserved also in the Rana catesbeiana mitochondrial genome.
3) The origin of the L-strand replication, which consists of 27 nucleotides, occurs in the middle of the five-tRNA gene cluster that is located between the genes for URF2 and CO I. The nucleotide sequence and potential secondary structure of this origin is very homologous to those in other higher vertebrates.
4) The seven mitochondrial tRNA genes of Rana catesbeiana show many structual features common to those of other higher vertebrates.抄録全体を表示PDF形式でダウンロード (756K) -
白 正善1987 年 54 巻 4 号 p. 441-452
発行日: 1987/08/15
公開日: 2009/07/10
ジャーナル フリーThe ultrastructural change in the anterior lobe of the pituitary gland of Wistar rats which were induced by severe thermal burns were investigated by electron microscope. The results obtained were as follows:
1) Swelling of the cell mitochondria, showing a characteristic vesicular form as seen in endo-crine organs, was noted immediately after the severe thermal bums were inflicted and the swelling continued for 48 hours. In the later stages, recovery from the swelling was noted. However, renewed swelling was observed 3 weeks after infliction of the burns. The change was very visible after 6 weeks. The change in the mitochondria corresponded to vascular stasis of the capillary vessels and disintegration'in the endothelial cells.
2) Six hours after the burns were inflicted abundant secretory granules were liberated form the cells of the anterior lobe and dispersed in the stroma of the perivascular spaces. Liberation of the ribosomes was also discernible in the stroma of the anterior lobe which suggests an intensive cellular injury induced by the shock of severe thermal burns.
Based on these observations it has been suggested that an increase in pituitary hormones at the initial stage of the shock would be a cardinal pathological process.
3) Liberation of secretory granules was confirmed, substantiating ultrastructural evidence of a hyperfunctional state of hormone secretion.
A similar change persisted until 6 weeks after infliction of the severe thermal burns, suggesting that an identical hyperfunctional state might last until this stage.
4) It was clear that the application of the Periodic Acid Methenamine Silver (PAM) Staining Procedure was very effective in evaluating the property of the cellular component of the endocrine system. Moreover, The PAM Staining Procedure was also effective in detecting the properties of the basement membrane as well as supporting connective tissue components.抄録全体を表示PDF形式でダウンロード (9716K) -
吉田 雄一郎1987 年 54 巻 4 号 p. 453-461
発行日: 1987/08/15
公開日: 2009/07/10
ジャーナル フリーMitochondriogenesis in the neonatal rat liver was studied with special reference to changes in the activity and content of cytochrome c oxidase, protein synthetic activity and the amounts of total protein and RNAs in mitochondria. The results obtained are as follows:
1) Cytochrome c oxidase was purified from the adult rat liver. The purified enzyme was resolved into seven constituent polypeptides upon SDS-polyacryamide gel electrophoresis and contained 14.3 nmol of heme a per mg of protein.
2) Immunotitration revealed that the enzyme could be quantitated with the antibody prepared against the holoenzyme, but not with those prepared against the subunits I (CO I) and II (CO II).
3) There was a marked increase in both the activity and immunologically quantitated amount of cytochrome c oxidase in the liver cell during neonatal development of the rat, giving a maximal value 3 days after birth. In contrast, the amount of total liver mitochondrial protein did not increase so rapidly.
4) The rate of protein synthesis in the isolated mitochondria from the neonatal rat liver was much higher than in those from the adult rat liver, also showing a maximal rate 3 days after birth.
5) The same patterns of increase were observed in the contents of total RNA, poly (A)+ RNA and mRNA for CO I, in terms of per mg mitochondrial protein, in the neonatal rat liver mitochondria.
The results imply that in the liver of the neonatal rat the synthesis of the mitochondrial innermembrane proteins is markedly stimulated and that this stimulation of protein synthesis is regulated at the level of transcription of the mitochondrial DNA.抄録全体を表示PDF形式でダウンロード (564K) -
堀内 淳一1987 年 54 巻 4 号 p. 462-466
発行日: 1987/08/15
公開日: 2009/07/10
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1987 年 54 巻 4 号 p. 467-469
発行日: 1987/08/15
公開日: 2009/07/10
ジャーナル フリーPDF形式でダウンロード (187K)
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